US4013418A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Luminescent reaction testing
Assignee: Vitatect Corp
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Filing: 1976-02-19
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Granted: 1977-03-22
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Expires: 1996-02-19
Abstract
Abstract An improved apparatus for testing a sample by means of luminescent reaction to detect reactive materials therein. A ribbon of filter material, which may be translucent or opaque, is provided to filter the sample and to carry the residue to a reaction position. A translucent surface is provided to carry a reagent to the reaction position. Both surfaces are brought into mutual contact in the optical vicinity of a photodetector, which thereby detects the resulting luminescent reaction to provide an indication of the quantity of reactive material present in the sample. An improved pinion roller advances the ribbon of filter material in a reliable fashion. In an alternative embodiment, the ribbon of filter material is used without the translucent surface.
Inventors
- 👤 Chris J. Plakas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:19
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/76; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10T; Y10T436/00; Y10T436/11; Y10T436/110833
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
161 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of filter material to produce a residue on the ribbon containing biological cells from the sample, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. advancing means coacting with the ribbon for advancing a portion of the ribbon of filter material containing the residue to a new position, D. liquid dispenser means for depositing liquid reagent directly on said portion of the ribbon of filter material containing the residue while said portion is in said new position, and E. photodetector means in optical proximity to the ribbon for detecting the intensity of a luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
Claim 2
Dependent Claim
161 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of filter material to produce a residue on the ribbon containing biological cells from the sample, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. advancing means coacting with the ribbon for advancing a portion of the ribbon of filter material containing the residue to a new position, D. liquid dispenser means for depositing liquid reagent directly on said portion of the ribbon of filter material containing the residue while said portion is in said new position, and E. photodetector means in optical proximity to the ribbon for detecting the intensity of a luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
Claim 3
Dependent Claim
32 words
A system according to claim 1 wherein the filtering means and the liquid dispenser means further comprise means for depositing the residue and the reagent on opposite sides of the filter material.
Claim 4
Dependent Claim
33 words
A system according to claim 1 wherein the filtering means and the liquid dispensing means further comprise means for depositing the residue and the reagent on the same side of the filter material.
Claim 5
Dependent Claim
24 words
A system according to claim 1 wherein the photodetector is located to detect the luminescent reaction while said portion is in said new position.
Claim 6
Dependent Claim
29 words
A system according to claim 1, wherein the extraction means comprises means for dispensing, as a liquid extractant, a solution of about 5% dioctyl sodium sulfosuccinate in distilled water.
Claim 7
Dependent Claim
13 words
A system according to claim 1 wherein the filter material is light transmitting.
Claim 8
Dependent Claim
12 words
A system according to claim 1 wherein the filter material is opaque.
Claim 9
Dependent Claim
19 words
A system according to claim 1 wherein the extraction means is an extractant material dispensed with the liquid reagent.
Claim 10
Dependent Claim
204 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of opaque filter material to produce a residue on the ribbon containing biological cells from the sample on one side of the filter material, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. an elongated tape of translucent material having a portion thereof positioned adjacent to the side of the ribbon of filter material on which said residue is deposited, D. advancing means coating with the ribbon and the tape for simultaneously advancing the ribbon and the tape, E. means for bringing reagent material into contact with the residue on the ribbon, and F. photodetector means situated in optical proximity to the ribbon and the tape on the side of the ribbon of opaque filter material on which the residue is deposited and separated from the residue by the tape of translucent material for detecting the luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
Claim 11
Dependent Claim
204 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of opaque filter material to produce a residue on the ribbon containing biological cells from the sample on one side of the filter material, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. an elongated tape of translucent material having a portion thereof positioned adjacent to the side of the ribbon of filter material on which said residue is deposited, D. advancing means coating with the ribbon and the tape for simultaneously advancing the ribbon and the tape, E. means for bringing reagent material into contact with the residue on the ribbon, and F. photodetector means situated in optical proximity to the ribbon and the tape on the side of the ribbon of opaque filter material on which the residue is deposited and separated from the residue by the tape of translucent material for detecting the luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
Claim 12
Dependent Claim
210 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of translucent filter material to produce a residue on the ribbon containing biological cells from the sample on one side of the filter material, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. an elongated tape of opaque material having a portion thereof positioned adjacent to the side of the ribbon of filter material on which said residue is deposited, D. advancing means coacting with the ribbon and the tape for simultaneously advancing the ribbon and the tape, E. means for bringing reagent material into contact with the residue on the ribbon, and F. photodetector means situated in optical proximity to the ribbon and the tape on the side of the ribbon of translucent filter material away from said one side on which the residue is deposited and separated from the residue by the ribbon of translucent filter material for detecting the luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
Claim 13
Dependent Claim
210 words
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising: A. filtering means for filtering said sample through an elongated ribbon of translucent filter material to produce a residue on the ribbon containing biological cells from the sample on one side of the filter material, B. extraction means operatively associated with the ribbon for rupturing the cells in the residue while on said ribbon of filter material, C. an elongated tape of opaque material having a portion thereof positioned adjacent to the side of the ribbon of filter material on which said residue is deposited, D. advancing means coacting with the ribbon and the tape for simultaneously advancing the ribbon and the tape, E. means for bringing reagent material into contact with the residue on the ribbon, and F. photodetector means situated in optical proximity to the ribbon and the tape on the side of the ribbon of translucent filter material away from said one side on which the residue is deposited and separated from the residue by the ribbon of translucent filter material for detecting the luminescent reaction which occurs when the reagent and the ruptured cells in the residue react together.
References Cited (Backward Citations)
US3690832A
Cited
US3940250A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-21 | 1980-09-20 | 1981-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-21 | 1984-09-20 | 1985-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-21 | 1988-09-20 | 1989-03-22 | $7700.00 | Window Closed / Lapsed |