US4013411A
Expired / Lapsed
US
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Method for detecting hepatitis associated antigen
Assignee: Villanova University
•
Filing: 1974-07-19
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Granted: 1977-03-22
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Expires: 1994-07-19
Abstract
Abstract A method for testing biological materials, and especially those obtained from the human body, for the presence of the hepatitis associated antigen (HAA), in which Zeise's salt, or another water soluble platinum or palladium salt containing a trans directing group which enables the noble metal-containing moiety of the salt to react with the hepatitis associated antigen (HAA) or related materials, is mixed with the material to be tested in the presence of water, causing the formation of characteristic visible turbidity in the mixture which persists after agitation if HAA is present. Any slight turbidity observed initially in normal sera disappears upon agitation if the test sample is free from the hepatitis associated antigen. Also disclosed are test reagents consisting of the platinum and palladium salts in aqueous solutions containing non-interfering stabilizing salts such as sodium and potassium chloride and equivalents.
Inventors
- 👤 Saul I. Shupack
- 👤 Stephen F. Malin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 23:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; Y; Y10; Y10S; Y10S436/00; Y10S436/82; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
118 words
A method for testing biological materials for the presence of the hepatitis associated antigen which comprises contacting a biological material to be tested, in the presence of water, with at least one water soluble reagent selected from the group consisting of cis, bis-triethylphosphine platinum (II) dichloride, cis, bis-triphenylphosphine hydrido platinum (II) chloride, and the anions ethylene platinum (II) trichloride, carbonyl platinum (II) trichloride, trans-2-butene platinum (II) trichloride, and isobutene platinum (II) trichloride, said anions being associated with a non-interfering cation; said contact producing a characteristic presistent precipitate detectable in the reaction mixture if the biological material contains the hepatitis associated antigen, whereas any precipitate formed initially is not persistent if the biological material is free of said antigen.
Claim 2
Independent Claim
118 words
A method for testing biological materials for the presence of the hepatitis associated antigen which comprises contacting a biological material to be tested, in the presence of water, with at least one water soluble reagent selected from the group consisting of cis, bis-triethylphosphine platinum (II) dichloride, cis, bis-triphenylphosphine hydrido platinum (II) chloride, and the anions ethylene platinum (II) trichloride, carbonyl platinum (II) trichloride, trans-2-butene platinum (II) trichloride, and isobutene platinum (II) trichloride, said anions being associated with a non-interfering cation; said contact producing a characteristic presistent precipitate detectable in the reaction mixture if the biological material contains the hepatitis associated antigen, whereas any precipitate formed initially is not persistent if the biological material is free of said antigen.
Claim 3
Dependent Claim
36 words
The method of claim 1 in which the biological material is a solid dissolved or suspended in water to form test sample having a clear aqueous phase in which the characteristic precipitate can be reliably detected.
Claim 4
Dependent Claim
14 words
The method of claim 2 in which the biological material is a stool sample.
Claim 5
Dependent Claim
13 words
The method of claim 2 in which the biological material is blood platelets.
Claim 6
Dependent Claim
12 words
The method of claim 2 in which the biological material is albumin.
Claim 7
Dependent Claim
15 words
The method of claim 2 in which the biological material is a frozen blood material.
Claim 8
Dependent Claim
15 words
The method of claim 2 in which the biological material is a blood clotting factor.
Claim 9
Dependent Claim
25 words
The method of claim 1 in which an aqueous solution of at least one of said reagents is mixed with the material to be tested.
Claim 10
Dependent Claim
20 words
The method of claim 8 wherein the aqueous reagent solution is stabilized with at least one non-interfering alkali metal salt.
Claim 11
Dependent Claim
21 words
The method of claim 9 wherein the aqueous reagent solution consists essentially of the reagent in solution in physiological saline solution.
Claim 12
Dependent Claim
14 words
The method of claim 10 wherein the reagent is potassium ethylene platinum (II) trichloride.
Claim 13
Dependent Claim
21 words
The method of claim 9 wherein the reagent is potassium ethylene platinum (II) trichloride and the biological material is ascites fluid.
Claim 14
Dependent Claim
14 words
The method of claim 1 wherein the biological material is a human blood material.
Claim 15
Dependent Claim
28 words
The method of claim 13 wherein the blood material is in liquid form and contains water and the reagent is potassium ethylene platinum (II) trichloride in solid form.
Claim 16
Dependent Claim
12 words
The method of claim 13 wherein the blood material is blood serum.
Claim 17
Dependent Claim
11 words
The method of claim 13 wherein the blood material is plasma.
Claim 18
Dependent Claim
12 words
The method of claim 13 wherein the blood material is gamma globulin.
Claim 19
Dependent Claim
25 words
The method of claim 13 wherein the blood material is in liquid form and the reagent is potassium ethylene platinum (II) trichloride in aqueous solution.
Claim 20
Dependent Claim
11 words
The method of claim 18 wherein the blood material is serum.
Claim 21
Dependent Claim
11 words
The method of claim 18 wherein the blood material is plasma.
Claim 22
Dependent Claim
12 words
The method of claim 18 wherein the blood material is gamma globulin.
Claim 23
Dependent Claim
23 words
The method of claim 8 wherein the aqueous solution contains from about 0.1 to about 0.5% potassium ethylene platinum (II) trichloride by weight.
Claim 24
Dependent Claim
31 words
The method of claim 8 wherein 200 μ1 of a 0.5% solution of potassium ethylene platinum (II) trichloride are added to a 200 μ1 sample of the material to be tested.
Claim 25
Dependent Claim
13 words
The method of claim 22 wherein the solution is stabilized with sodium chloride.
Claim 26
Dependent Claim
13 words
The method of claim 22 wherein the solution is stabilized with potassium chloride.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-21 | 1980-09-20 | 1981-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-21 | 1984-09-20 | 1985-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-21 | 1988-09-20 | 1989-03-22 | $7700.00 | Window Closed / Lapsed |