US4012475A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for sectioning in a microtome at low temperature and trimming of the specimen to be sectioned
Assignee: Reichert Jung Optische Werke AG
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Filing: 1974-08-23
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Granted: 1977-03-15
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Expires: 1994-08-23
Abstract
Abstract According to the invention the specimen is given its desired design by pressing it towards a block, having a recess of the desired design and being cooled, preferably by liquid nitrogen.
Inventors
- 👤 Erik Lennart Kindel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 16:45
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/02; G01N1/04; G01N1/06; G01N1/28; G01N1/42; Y; Y10; Y10T; Y10T83/00; Y10T83/283
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
85 words
Method of preparing a specimen for sectioning by a microtome at low temperature comprising the steps of: a. bringing a specimen into contact with a rod; b. adhering the specimen to said rod; c. manipulating the rod to convey the adhered specimen into contact with a cooled block, said block being provided with a recess having the shape desired to be imparted to said specimen; d. and pressing at least a part of said specimen into said recess to freeze said part in said shape.
Claim 2
Dependent Claim
85 words
Method of preparing a specimen for sectioning by a microtome at low temperature comprising the steps of: a. bringing a specimen into contact with a rod; b. adhering the specimen to said rod; c. manipulating the rod to convey the adhered specimen into contact with a cooled block, said block being provided with a recess having the shape desired to be imparted to said specimen; d. and pressing at least a part of said specimen into said recess to freeze said part in said shape.
Claim 3
Dependent Claim
14 words
Method of claim 1 which includes the step of cooling said block with nitrogen.
Claim 4
Dependent Claim
18 words
Method of claim 1, which includes the step of at least partially submerging said block in liquid nitrogen.
References Cited (Backward Citations)
US3495490A
Cited
US3611875A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-14 | 1980-09-13 | 1981-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-14 | 1984-09-13 | 1985-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-14 | 1988-09-13 | 1989-03-15 | $7700.00 | Window Closed / Lapsed |