US4011377A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme separation
Assignee: Individual
•
Filing: 1975-09-04
•
Granted: 1977-03-08
•
Expires: 1995-09-04
Abstract
Abstract A reactive matrix comprises a co-enzyme chemically attached to a water insoluble organic polymeric support material. The reactive matrix may be used in a process for the separation of mixtures containing a plurality of enzymes.
Inventors
- 👤 Peter Duncan Goodearl Dean
- 👤 Christopher Robin Lowe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:36
CPC Classifications
C; C12; C12N; C12N9/00; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
78 words
A reactive matrix consisting essentially of adenosine triphosphate chemically attached to a water insoluble organic polymeric support material, said reactive matrix being free from chemically attached enzymes, and said water insoluble organic polymeric support material containing said adenosine triphosphate attached thereto being capable of attracting enzymes in affinity chromatography which are attracted to said adenosine triphosphate, thereby making it possible to separate enzymes from other components of a liquid medium containing said enzymes as well as other enzymes.
Claim 2
Dependent Claim
78 words
A reactive matrix consisting essentially of adenosine triphosphate chemically attached to a water insoluble organic polymeric support material, said reactive matrix being free from chemically attached enzymes, and said water insoluble organic polymeric support material containing said adenosine triphosphate attached thereto being capable of attracting enzymes in affinity chromatography which are attracted to said adenosine triphosphate, thereby making it possible to separate enzymes from other components of a liquid medium containing said enzymes as well as other enzymes.
Claim 3
Dependent Claim
15 words
A matrix as claimed in claim 1 wherein the organic polymeric support material is hydrophilic.
Claim 4
Dependent Claim
30 words
A matrix as claimed in claim 1 wherein the organic polymeric support material is in the form of a permeable material which is adapted to make up a compact column.
Claim 5
Dependent Claim
30 words
A matrix as claimed in claim 1 wherein the organic polymeric support material is a polymer containing a plurality of vicinal diol groups which has been reacted with cyanogen bromide.
Claim 6
Dependent Claim
46 words
A matrix as claimed in claim 1 in which the adenosine triphosphate is linked to the backbone of the polymer chain of the organic polymeric support material by an intermediate carbon chain containing 2 to 20 carbon atoms or by an oligopeptide or a polyaromatic system.
Claim 7
Dependent Claim
15 words
A matrix as claimed in claim 1 wherein the organic polymeric support material is polyacrylamide.
Claim 8
Dependent Claim
53 words
A matrix as claimed in claim 1 wherein the organic polymeric support material is selected from the group consisting of polyacrylamide, cellulose acetate, a starch, a dextran, a protein, agarose, polyvinyl alcohol, nylon, a polyester, chloromethylated polystyrenes and polymers containing a plurality of vicinal diol groups which have been reacted with cyanogen bromide.
Claim 9
Dependent Claim
13 words
A matrix as claimed in claim 4 in which said polymer is cellulose.
Claim 10
Dependent Claim
16 words
A matrix as claimed in claim 1 in which the organic polymeric support material is agarose.
References Cited (Backward Citations)
US3935072A
Cited
US3947352A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-07 | 1980-09-06 | 1981-03-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-07 | 1984-09-06 | 1985-03-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-07 | 1988-09-06 | 1989-03-08 | $7700.00 | Window Closed / Lapsed |