US4010072A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing L-tartaric acid
Assignee: Tokuyama Corp
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Filing: 1976-02-05
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Granted: 1977-03-01
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Expires: 1996-02-05
Abstract
Abstract A process for preparing L-tartaric acid, which comprises contacting a microorganism of the genus Nocardia having the ability to produce an enzyme which cleaves and hydrolyzes the epoxy ring of cis-epoxysuccinic acid, or an enzyme isolated therefrom, with cis-epoxysuccinic acid or its derivatives in an aqueous medium, and recovering the resulting L-tartaric acid.
Inventors
- 👤 Yuichi Miura
- 👤 Kiyohiko Yutani
- 👤 Yusuke Izumi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 07:19
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12P; C12P7/00; C12P7/40; C12P7/44; C12P7/46; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/872
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
54 words
Cis-epoxysuccinate hydrolase obtained from Nocardia tartaricans characterized by (1) a molecular weight of from 60,000 to 95,000, (2) an optimum pH as illustrated in FIG. 2, (3) an optimum temperature as illustrated in FIG. 3, (4) a pH stability as illustrated in FIG. 4, and (5) a temperature stability as illustrated in FIG. 5.
Claim 2
Dependent Claim
54 words
Cis-epoxysuccinate hydrolase obtained from Nocardia tartaricans characterized by (1) a molecular weight of from 60,000 to 95,000, (2) an optimum pH as illustrated in FIG. 2, (3) an optimum temperature as illustrated in FIG. 3, (4) a pH stability as illustrated in FIG. 4, and (5) a temperature stability as illustrated in FIG. 5.
Claim 3
Independent Claim
51 words
A method of producing the enzyme cis-epoxysuccinate hydrolase which comprises culturing a microorganism belonging to the species Nocardia tartaricans capable of producing said enzyme in a nutrient medium containing assimilable sources of carbon and nitrogen until a microbial population is developed; harvesting said microbial cells and thereafter isolating said enzyme therefrom.
Claim 4
Independent Claim
51 words
A method of producing the enzyme cis-epoxysuccinate hydrolase which comprises culturing a microorganism belonging to the species Nocardia tartaricans capable of producing said enzyme in a nutrient medium containing assimilable sources of carbon and nitrogen until a microbial population is developed; harvesting said microbial cells and thereafter isolating said enzyme therefrom.
Claim 5
Dependent Claim
16 words
A method according to claim 2 wherein said microorganism is Nocardia tartaricans nov. sp. ATCC 31190.
Claim 6
Dependent Claim
16 words
A method according to claim 2 wherein said microorganism is Nocardia tartaricans nov. sp. ATCC 31191.
Claim 7
Dependent Claim
42 words
A method according to claim 2 wherein said enzyme is isolated by disrupting said microbial cells to obtain a crude cell-free extract; fractionating said extract by precipitation with ammonium sulfate; dialyzing said precipitate and then purifying by column chromatography and gel filtration.
Claim 8
Independent Claim
65 words
A method for preparing L-tartaric acid which comprises reacting cells of a microorganism belonging to the genus Nocardia having the ability to produce an enzyme which hydrolyzes the epoxy ring of cis-epoxysuccinic acid or cis-epoxysuccinic hydrolase derived from said microorganism with cis-epoxysuccinic acid or a derivative thereof having the cis-epoxysuccinic acid structure in an aqueous medium, and thereafter recovering L-tartaric acid from said aqueous medium.
Claim 9
Independent Claim
65 words
A method for preparing L-tartaric acid which comprises reacting cells of a microorganism belonging to the genus Nocardia having the ability to produce an enzyme which hydrolyzes the epoxy ring of cis-epoxysuccinic acid or cis-epoxysuccinic hydrolase derived from said microorganism with cis-epoxysuccinic acid or a derivative thereof having the cis-epoxysuccinic acid structure in an aqueous medium, and thereafter recovering L-tartaric acid from said aqueous medium.
Claim 10
Dependent Claim
15 words
A method according to claim 6 wherein said microorganism belongs to the species Nocardia tartaricans.
Claim 11
Dependent Claim
29 words
A method according to claim 7 wherein said microorganism is selected from the group consisting of Nocardia tartaricans nov. sp. ATCC 31190 and Nocardia tartaricans nov. sp. ATCC 31191.
Claim 12
Dependent Claim
25 words
A method according to claim 6 wherein said reaction step is carried out at a temperature of from about 20° C. to about 60° C.
Claim 13
Dependent Claim
22 words
A method according to claim 6 wherein said reaction step is carried out at a pH of about 6 to about 10.
Claim 14
Dependent Claim
21 words
A method according to claim 6 wherein said reaction step is carried out by culturing said microorganism in said aqueous medium.
Claim 15
Dependent Claim
26 words
A method according to claim 6 wherein said reaction step is carried out using a culture broth or a cell free extract containing said cis-epoxysuccinate hydrolase.
Claim 16
Dependent Claim
21 words
A method according to claim 6 wherein said reaction step is carried out using dried or pulverized cells of said microorganism.
Claim 17
Independent Claim
61 words
A method for preparing L-tartaric acid which comprises culturing a microorganism belonging to the genus Nocardia having the ability to produce an enzyme which hydrolyzes the epoxy ring of cis-epoxysuccinic acid in an aqueous medium containing cis-epoxysuccinic acid or a derivative thereof having the cis-epoxysuccinic acid structure until L-tartaric acid is formed in said medium and thereafter recovering said L-tartaric acid.
Claim 18
Independent Claim
61 words
A method for preparing L-tartaric acid which comprises culturing a microorganism belonging to the genus Nocardia having the ability to produce an enzyme which hydrolyzes the epoxy ring of cis-epoxysuccinic acid in an aqueous medium containing cis-epoxysuccinic acid or a derivative thereof having the cis-epoxysuccinic acid structure until L-tartaric acid is formed in said medium and thereafter recovering said L-tartaric acid.
Claim 19
Dependent Claim
29 words
A method according to claim 14 wherein said microorganism is selected from the group consisting of Nocardia tartaricans nov. sp. ATCC 31190 and Nocardia tartaricans nov. sp. ATCC 31191.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-29 | 1980-08-30 | 1981-03-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-29 | 1984-08-30 | 1985-03-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-29 | 1988-08-30 | 1989-03-01 | $7700.00 | Window Closed / Lapsed |