US4009079A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for quantitatively determining glucoamylase in human urine and body fluids
Assignee: Ono Pharmaceutical Co Ltd
•
Filing: 1976-01-26
•
Granted: 1977-02-22
•
Expires: 1996-01-26
Abstract
Abstract A method for quantitatively determining glucoamylase in human urine and body fluids which comprises removing low molecular weight substances from a human urine or body fluid sample, adding to the resulting sample which is free of low molecular weight (from about 500 to about 20,000) substances maltotriitol, phenyl- alpha -glucoside or 2,4-dinitrophenyl- alpha -glucoside, which are specific substrates for glucoamylase, and quantitatively determining the glucose, phenol or 2,4-dinitrophenol which is a degradation product of the above substrates, respectively.
Inventors
- 👤 Kazuaki Tsujino
- 👤 Junnosuke Kida
- 👤 Masanobu Hosotani
- 👤 Noshi Minamiura
- 👤 Takehiko Yamamoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 15:30
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/40; C12Q2334/00
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
75 words
A method for determining the amount of glucoamylase in human urine and body fluids which comprises removing low molecular weight substances having a molecular weight of from about 500 to about 20,000 from a human urine or body fluid sample, adding to the resulting sample which is free of low molecular weight substances maltotriitol which is a specific substrate of glucoamylase, and measuring the amount of glucose, which is the degradation product of the substrate.
Claim 2
Independent Claim
75 words
A method for determining the amount of glucoamylase in human urine and body fluids which comprises removing low molecular weight substances having a molecular weight of from about 500 to about 20,000 from a human urine or body fluid sample, adding to the resulting sample which is free of low molecular weight substances maltotriitol which is a specific substrate of glucoamylase, and measuring the amount of glucose, which is the degradation product of the substrate.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-22 | 1980-08-23 | 1981-02-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-22 | 1984-08-23 | 1985-02-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-22 | 1988-08-23 | 1989-02-22 | $7700.00 | Window Closed / Lapsed |