US4006350A
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Method for performing a separating analysis
Assignee: Czech Academy of Sciences
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Filing: 1975-04-03
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Granted: 1977-02-01
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Expires: 1995-04-03
Abstract
Abstract A separating analysis, particularly a continuous quick chromatographic analysis is performed by introduction of doses of the sample to be analyzed into a separating column at pseudorandom sequences and by detecting concentrations of components of the sample at the output of the separating device at a rhytmus of the dosing orders and evaluating the separating analysis by summarizing the read values.
Inventors
- 👤 Jan Jokl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 10:53
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/62
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
208 words
A method for performing a separating analysis, wherein the sample to be analyzed is dosed into a separating device and a signal generated in response thereto, corresponding to the concentration of individual components of the sample at the outlet of the separating device within certain time intervals, comprising the steps of dosing the sample in a regular rhytmus at equal doses in pseudorandom sequences according to a program for dosing comprising a recorded set of binary instructions, containing a given number of x = 2 n - 1 orders, where n is an integer positive number and the orders are of a binary character, the time of duration of one pseudorandom sequence being determined by the rhytmus and mumber of orders being chosen at least equal to the time of duration of the passage of the slowest component through the separating device, the instantaneous values of the output signal, proportional to the instantaneous summary concentrations of components of the sample being read by a detector in a rhytmus proportional to the rhytmus of dosing orders, the separating analysis being thereafter carried out based on summation of the read values by a control program comprising a recorded set of binary instructions derived from the program for dosing the sample.
Claim 2
Independent Claim
208 words
A method for performing a separating analysis, wherein the sample to be analyzed is dosed into a separating device and a signal generated in response thereto, corresponding to the concentration of individual components of the sample at the outlet of the separating device within certain time intervals, comprising the steps of dosing the sample in a regular rhytmus at equal doses in pseudorandom sequences according to a program for dosing comprising a recorded set of binary instructions, containing a given number of x = 2 n - 1 orders, where n is an integer positive number and the orders are of a binary character, the time of duration of one pseudorandom sequence being determined by the rhytmus and mumber of orders being chosen at least equal to the time of duration of the passage of the slowest component through the separating device, the instantaneous values of the output signal, proportional to the instantaneous summary concentrations of components of the sample being read by a detector in a rhytmus proportional to the rhytmus of dosing orders, the separating analysis being thereafter carried out based on summation of the read values by a control program comprising a recorded set of binary instructions derived from the program for dosing the sample.
Claim 3
Dependent Claim
31 words
The method according to claim 1 wherein the amount of the sample of one dose is chosen proportional to the sensitivity of the detector in the range of its linear response.
Claim 4
Dependent Claim
22 words
The method according to claim 1, wherein the number x of said orders is chosen according to the required fineness of separation.
Claim 5
Dependent Claim
67 words
The method according to claim 1, wherein the number of summations is equal at least to the sum of chosen components of the sample and to the chosen number of reference summations and at the most to the number of orders of one pseudorandom sequence, the result of the first summation being chosen as a main reference summation used as an internal standard of the separating analysis.
Claim 6
Dependent Claim
28 words
The method according to claim 1, wherein the result of the last summation is chosen as an auxiliary reference summation in order to check the finished separating process.
Claim 7
Dependent Claim
106 words
The method according to claim 1 where when evaluating the separating analysis, the values of the output signal of the detector, proportional to the sum of instantaneous concentrations of components of the sample, read in a rhytmus proportional to the rhytmus of dosing orders, are added at the first summation according to the instantaneous binary state of the fundamental pseudorandom sequence x, whereby for the second, third, fourth to x th summation they are added according to the instantaneous binary state of the pseudorandom sequence drived from the fundamental sequence, contained in the program for dosing by cyclical shift by one, two, three to (x-1) positions.
Claim 8
Dependent Claim
29 words
The method according to claim 1, the concentration of the second to x th component of the sample being derived from results of the second to x th summation.
Claim 9
Dependent Claim
140 words
Apparatus for performing a separation analysis, comprising a separating column, a dosing device for dosing the sample to be analyzed connected to its inlet, a generator of pseudorandom sequences of orders for the dosing device, a detector connected to the output of the separating column, an amplifier, an analog-digital converter, a number of gates and counters, the control inlet of the dosing device connected with the first output of the generator of pseudorandom sequences of order, the output of the detector connected to the input of the amplifier, the output of which is connected with the input of the analog-digital converter, the output of which is connected with inputs of electronic gates, the control inputs of which are connected with second outputs of the generator of pseudorandom sequences of orders, the outputs of the gates connected with inputs of counters.
Claim 10
Dependent Claim
140 words
Apparatus for performing a separation analysis, comprising a separating column, a dosing device for dosing the sample to be analyzed connected to its inlet, a generator of pseudorandom sequences of orders for the dosing device, a detector connected to the output of the separating column, an amplifier, an analog-digital converter, a number of gates and counters, the control inlet of the dosing device connected with the first output of the generator of pseudorandom sequences of order, the output of the detector connected to the input of the amplifier, the output of which is connected with the input of the analog-digital converter, the output of which is connected with inputs of electronic gates, the control inputs of which are connected with second outputs of the generator of pseudorandom sequences of orders, the outputs of the gates connected with inputs of counters.
Claim 11
Dependent Claim
22 words
The apparatus according to claim 8, wherein the input of an analog recording device is connected to the output of the amplifier.
Claim 12
Dependent Claim
30 words
The apparatus according to claim 8, including a multichannel digital recording device connected to the output of the analog-digital converter and to the outputs of the generator of pseudorandom sequences.
Claim 13
Dependent Claim
43 words
The apparatus according to claim 8, wherein the source of pseudorandom sequences comprises a rotor of light impervious material provided with openings and a stator provided with photoelectric indicators of said openings in the rotor, whereby the photoelectric indicators are mutually relatively shiftable.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-01 | 1980-08-02 | 1981-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-01 | 1984-08-02 | 1985-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-01 | 1988-08-02 | 1989-02-01 | $7700.00 | Window Closed / Lapsed |