US4006057A
Expired / Lapsed
US
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Method of producing L-cysteine and L-cystine
Assignee: Ajinomoto Co Inc
•
Filing: 1975-11-04
•
Granted: 1977-02-01
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Expires: 1995-11-04
Abstract
Abstract A method for producing L-cysteine and/or L-cystine which comprises: A. holding 2-amino-thiazoline-4-carboxylic acid in an aqueous solution at a pH of 5 to 11 in the presence of an effective amount of enzyme produced by a microorganism; said enzyme being capable of converting the 2-amino-thiazoline-4-carboxylic acid to L-cysteine and/or L-cystine, and said microorganism being capable of growing in a medium containing 2-amino-thiazoline-4-carboxylic acid as a nitrogen source; and B. recovering the L-cysteine and/or L-cystine formed from said aqueous solution.
Inventors
- 👤 Konosuke Sano
- 👤 Keizo Matsuda
- 👤 Koji Mitsugi
- 👤 Kazuhiko Yamada
- 👤 Fumihide Tamura
- 👤 Naohiko Yasuda
- 👤 Ichiro Noda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:55
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/12; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/824; Y10S435/829; Y10S435/832; Y10S435/833; Y10S435/84; Y10S435/847; Y10S435/859; Y10S435/874; Y10S435/88; Y10S435/881
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
83 words
A method for producing L-cysteine and/or L-cystine which comprises: a. holding 2-amino-thiazoline-4-carboxylic acid in an aqueous solution at a pH of 5 to 11 in the presence of an effective amount of enzyme produced by a microorganism; said enzyme being capable of converting 2-amino-thiazoline-4-carboxylic acid to L-cysteine and/or L-cystine, and said microorganism being capable of growing in a medium containing 2-amino-thiazoline-4-carboxylic acid as a nitrogen source, and of producing said enzyme; and b. recovering the L-cysteine and/or L-cystine formed from said aqueous solution.
Claim 2
Independent Claim
83 words
A method for producing L-cysteine and/or L-cystine which comprises: a. holding 2-amino-thiazoline-4-carboxylic acid in an aqueous solution at a pH of 5 to 11 in the presence of an effective amount of enzyme produced by a microorganism; said enzyme being capable of converting 2-amino-thiazoline-4-carboxylic acid to L-cysteine and/or L-cystine, and said microorganism being capable of growing in a medium containing 2-amino-thiazoline-4-carboxylic acid as a nitrogen source, and of producing said enzyme; and b. recovering the L-cysteine and/or L-cystine formed from said aqueous solution.
Claim 3
Dependent Claim
27 words
The method of in claim 1, wherein said microorganism belongs to the genus Sarcina, Achromobacter, Alcaligenes, Bacillus, Brevibacterium, Enterobacter, Ervinia, Escherichia, Micrococcus, Mycoplana, Serratia, Flavobacterium, or Pseudomonas.
Claim 4
Dependent Claim
43 words
A method of claim 1, wherein said microorganism belongs to the species: Sarcina lutea Achromobacter delmarvae Alcaligenes denitrificans Bacillus brevis Brevibacterium flavum Enterobacter aerogenes Ervinia carotovora Escherichia coli Micrococcus sodonensis Mycoplana dimorpha Serratia marcescens Pseudomonas thiazolinophilum Pseudomonas ovalis Pseudomonas desmolytica or Pseudomonas cohaerens
Claim 5
Dependent Claim
94 words
A method of claim 1, wherein said microorganism is: ______________________________________
Sarcina lutea ATCC 272
Achromobacter delmarvae
FERM-P 21
Alcaligenes denitrificans
ATCC 15173
Bacillus brevis ATCC 8185
Brevibacterium flavum
ATCC 13826
Enterobacter aerogenes
FERM-P 2764
Ervinia carotovora
FERM-P 2766
Escherichia coli FERM-P 2763
Micrococcus sodonensis
ATCC 11880
Mycoplana dimorpha
ATCC 4279
Serratia marcescens
FERM-P 2765
Pseudomonas thiazolinophilum
FERM-P 2810
Pseudomonas ovalis
FERM-P 2762, FERM-P 2811,
FERM-P 2812, FERM-P 2813,
FERM-P 2814, or FERM-P 2815
Pseudomonas desmolytica
FERM-P 2816, FERM-P 2817
FERM-P 2818, FERM-P 2819,
FERM-P 2820 or FERM-P 2821
or
Pseudomonas cohaerens
FERM-P 2831.
______________________________________
Claim 6
Dependent Claim
49 words
A method of claim 1, wherein said microorganism is capable of growing in a medium containing, per deciliter, 0.3 g 2-amino-thiazoline-4-carboxylic acid.sup.. trihydrate, 1 g glycerol, 0.01 g yeast extract, 0.1 g KH 2 PO 4 , 0.05 g MgSo 4 .sup.. 7H 2 O, and 2 g agar.
Claim 7
Dependent Claim
20 words
A method of claim 1, wherein said enzyme is produced by culturing said microorganism in a medium containing 2-amino-thiazoline-4-carboxylic acid.
Claim 8
Dependent Claim
26 words
A method of claim 1, wherein said enzyme is produced by culturing in a medium containing manganese ions in an amount of more than 0.1 mM.
Claim 9
Dependent Claim
26 words
A method of claim 1, wherein said enzyme is produced by culturing in a medium containing ferrous ions in an amount of more than 0.1 mM.
Claim 10
Dependent Claim
39 words
The method of claim 1, wherein intact cells of said microorganism, a homogenate of the cells of said microorganism, freeze-dried cells of said microorganism, or a sonicate of the cells of said microorganism are used as the enzyme source.
Claim 11
Dependent Claim
27 words
The method of claim 1, wherein the cells of said microorganism which are used as the source of said enzyme have been contacted with an organic solvent.
Claim 12
Dependent Claim
22 words
The method of claim 10, wherein said organic solvent is a lower alkanol, an aromatic hydrocarbon, an alkylketone, or a chlorinated alkane.
Claim 13
Dependent Claim
26 words
The method of claim 1, wherein the cells of said microorganism which are used as the source of said enzyme have been contacted with a surfactant.
Claim 14
Dependent Claim
16 words
The method of claim 12, wherein said surfactant is a cationic surfactant or an anionic surfactant.
Claim 15
Dependent Claim
30 words
The method of claim 1, wherein the cells of said microorganism which are used as the source of said enzyme have been treated with a lytic enzyme of microbial cells.
Claim 16
Dependent Claim
13 words
A method of claim 1, wherein said aqueous solution contains hydroxylamine or semicarbazide.
Claim 17
Dependent Claim
20 words
A method of claim 1, wherein said aqueous solution contains ferrous ions in an amount of more than 0.036 mM.
Claim 18
Dependent Claim
20 words
A method of claim 1, wherein said aqueous solution contains ferric ions in an amount of more than 0.036 mM.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-01 | 1980-08-02 | 1981-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-01 | 1984-08-02 | 1985-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-01 | 1988-08-02 | 1989-02-01 | $7700.00 | Window Closed / Lapsed |