US4004981A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell removing device
Assignee: Merck and Co Inc
•
Filing: 1975-05-01
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Granted: 1977-01-25
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Expires: 1995-05-01
Abstract
Abstract Apparatus for physically removing cells in sterile manner from a disc stack on which the cells have been cultured comprises a substantially shaft-like member having at least one rod-like member mounted at substantially a right angle to the axis of the shaft.
Inventors
- 👤 William M. Hurni
- 👤 William J. McAleer
- 👤 Maurice R. Hilleman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:35
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/06; C12M27/00; C12M27/14; C12M33/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
97 words
In a multiplate cell propagator comprising a substantially cylindrical vessel having a plurality of spaced apart parallel discs on which cells are grown, the discs mounted on a rotatable shaft within the vessel, the improvement comprising a second shaft pivotably mounted within the vessel, the second shaft having mounted thereon parallel to the plane of the discs a contacting means adapted to contact at least part of the surface of a disc upon pivotal movement of the second shaft whereby cells are removed from the surface of the disc, the contacting means comprising a substantially rod-like member.
Claim 2
Dependent Claim
97 words
In a multiplate cell propagator comprising a substantially cylindrical vessel having a plurality of spaced apart parallel discs on which cells are grown, the discs mounted on a rotatable shaft within the vessel, the improvement comprising a second shaft pivotably mounted within the vessel, the second shaft having mounted thereon parallel to the plane of the discs a contacting means adapted to contact at least part of the surface of a disc upon pivotal movement of the second shaft whereby cells are removed from the surface of the disc, the contacting means comprising a substantially rod-like member.
Claim 3
Dependent Claim
18 words
Apparatus according to claim 1 wherein the rod-like member is coated with a flexible, substantially inert, impermeable material.
Claim 4
Dependent Claim
29 words
Apparatus according to claim 1 wherein the rod-like member is covered with a tubing of flexible, substantially inert, impermeable material bonded to the rod-like member by a flexible adhesive.
Claim 5
Dependent Claim
11 words
Apparatus according to claim 3 wherein the tubing is polyfluorinated hydrocarbon.
Claim 6
Dependent Claim
22 words
Apparatus according to claim 1 wherein the second shaft has a substantially flat surface from which the substantially rod-like member is mounted.
Claim 7
Dependent Claim
20 words
Apparatus according to claim 5 wherein the rod-like member is mounted in a recess provided in the substantially flat surface.
Claim 8
Dependent Claim
21 words
Apparatus according to claim 5 wherein the second shaft has a hollow portion adapted to be fitted over a guide rod.
Claim 9
Dependent Claim
12 words
Apparatus according to claim 5 having a plurality of substantially rod-like members.
Claim 10
Dependent Claim
65 words
A method for physically removing cells from a plurality of discs mounted on a central shaft on which discs the cells have been cultured comprising the steps of positioning apparatus according to claim 1 so that the substantially rod-like member is between adjacent discs and rotating the second shaft so that the rod-like member contacts at least part of the surface of an adjacent disc.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-25 | 1980-07-26 | 1981-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-25 | 1984-07-26 | 1985-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-25 | 1988-07-26 | 1989-01-25 | $7700.00 | Window Closed / Lapsed |