US4004974A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Live virus culture vaccine against carnivore distemper and method of producing same
Assignee: Individual
•
Filing: 1975-05-07
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Granted: 1977-01-25
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Expires: 1995-05-07
Abstract
Abstract A live virus culture vaccine against carnivore distemper. Said vaccine is an attenuated strain of the carnivore distemper virus adapted to a cell culture of the kidney tissue of green monkeys and cultivated in said culture. The method of producing said vaccine comprises infecting a cell culture of the kidney tissue of green monkeys with a carnivore distemper virus strain adapted to said culture and cultivating it in said culture. The advantages offered by the proposed vaccine are standard properties, safety, adequate immunological activity and epizootological effectiveness as well as low production cost.
Inventors
- 👤 Mikhail Petrovich Chumakov
- 👤 Inna Alexandrovna Prokhorova
- 👤 Viktor Pavlovich Grachev
- 👤 Valentina Vasilievna Petukhova
- 👤 Julia Anatolievna Svezhinina
- 👤 Ljubov Leonidovna Mironova
- 👤 Ninel Mikhailovna Ralf
- 👤 Valentina Dmitrievna Popova
- 👤 Aida Nurislamovna Mustafina
- 👤 Vladimir Ivanovich Chernyshev
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:50
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/155; A61K39/175; A61K2039/51; A61K2039/525; A61K2039/5254; A61K2039/55; A61K2039/552; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/18011; C12N2760/18411; C12N2760/18434; Y; Y10; Y10S; Y10S424/00; Y10S424/818; Y10S424/821
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
134 words
A live virus culture vaccine useful against carnivore distemper and which is safe for animals, prepared by the sequential steps of (1) pre-adapting an attenuated strain of carnivore distemper virus to a cell culture of monkey (green monkey) kidney tissue by 8 to 10 passages at a temperature of 34(± 0.2)C°, the incubation period for viral growth ranging from 4 to 5 days; (2) cultivating the adapted strain in the cell culture of monkey (green monkey) kidney tissue, which cell culture is selected from among a primary cell culture or a subculture, in the presence of a nutrient medium at a temperature of 34(±0.2)C°; (3) removing the resultant virus-containing fluid of the degenerated cell detritus of said culture; and (4) lyophilizing the liquid vaccine thus formed in the presence of a suitable virus thermostabilizer.
Claim 2
Dependent Claim
134 words
A live virus culture vaccine useful against carnivore distemper and which is safe for animals, prepared by the sequential steps of (1) pre-adapting an attenuated strain of carnivore distemper virus to a cell culture of monkey (green monkey) kidney tissue by 8 to 10 passages at a temperature of 34(± 0.2)C°, the incubation period for viral growth ranging from 4 to 5 days; (2) cultivating the adapted strain in the cell culture of monkey (green monkey) kidney tissue, which cell culture is selected from among a primary cell culture or a subculture, in the presence of a nutrient medium at a temperature of 34(±0.2)C°; (3) removing the resultant virus-containing fluid of the degenerated cell detritus of said culture; and (4) lyophilizing the liquid vaccine thus formed in the presence of a suitable virus thermostabilizer.
Claim 3
Independent Claim
137 words
A method for preparing a live virus culture vaccine useful against carnivore distemper and which is safe for animals, which comprises the sequential steps of (1) pre-adapting an attenuated strain of carnivore distemper virus to a cell culture of monkey (green monkey) kidney tissue by 8 to 10 passages at a temperature of 34(±0.2)C°, the incubation period for viral growth ranging from 4 to 5 days; (c) cultivating the adapted strain in the cell culture of monkey (green monkey) kidney tissue, which cell culture is selected from among a primary cell culture or a subculture, in the presence of a nutrient medium at a temperature of 34(±0.2)C°; (3) removing the resultant virus-containing fluid of the degenerated cell detritus of said culture; and (4) lyophilizing the liquid vaccine thus formed in the presence of a suitable virus thermostabilizer.
Claim 4
Independent Claim
137 words
A method for preparing a live virus culture vaccine useful against carnivore distemper and which is safe for animals, which comprises the sequential steps of (1) pre-adapting an attenuated strain of carnivore distemper virus to a cell culture of monkey (green monkey) kidney tissue by 8 to 10 passages at a temperature of 34(±0.2)C°, the incubation period for viral growth ranging from 4 to 5 days; (c) cultivating the adapted strain in the cell culture of monkey (green monkey) kidney tissue, which cell culture is selected from among a primary cell culture or a subculture, in the presence of a nutrient medium at a temperature of 34(±0.2)C°; (3) removing the resultant virus-containing fluid of the degenerated cell detritus of said culture; and (4) lyophilizing the liquid vaccine thus formed in the presence of a suitable virus thermostabilizer.
Claim 5
Dependent Claim
32 words
The method of claim 2, wherein the cultivation of the carnivore distemper virus is carried out in a cell culture of monkey (green monkey) kidney tissue reconstituted following storage in liquid nitrogen.
Claim 6
Dependent Claim
59 words
The method of claim 2, wherein, in order to increase the yield of the carnivore distemper virus and to achieve a more complete use of the cell culture in cultivating the strain, at least a 3-fold replacement of the virus-containing fluid is carried out by a fresh nutrient medium to the point of total cell degradation of said culture.
Claim 7
Dependent Claim
30 words
The method of claim 4, wherein the virus-containing fluid is removed for the first time after approximately 30 to 40% of the cells of said culture exhibit a cytopathic effect.
Claim 8
Dependent Claim
36 words
The method of claim 2, wherein the virus-containing fluid is lyophilized in the presence of 4.5 to 5.5% sorbite by weight and 1.4 to 1.6% gelatose by weight, said sorbite and said gelatose serving as thermostabilizers.
References Cited (Backward Citations)
US3836626A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-25 | 1980-07-26 | 1981-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-25 | 1984-07-26 | 1985-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-25 | 1988-07-26 | 1989-01-25 | $7700.00 | Window Closed / Lapsed |