US4003789A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Triglyceride-accumulating clonal cell line
Assignee: Harvard University
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Filing: 1975-01-08
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Granted: 1977-01-18
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Expires: 1995-01-08
Abstract
Abstract An isolated clonal cell line derived from the mouse fibroblast 3T3 line is disclosed. This 3T3-L1 clonal cell line has the unique characteristic of accumulating large amounts of triglyceride fats in its resting state, which makes it useful for screening drugs to determine their effect on cell fat accumulation.
Inventors
- 👤 Howard Green
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:45
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0656; C12N2503/00; C12N2503/02
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
41 words
An isolated clonal cell line derived from a culture of mouse fibroblast 3T3 cell line, said clonal cell line having the characteristic of accumulating relatively large amounts of triglyceride fats while in a resting state and a suitable growth media therefrom.
Claim 2
Dependent Claim
41 words
An isolated clonal cell line derived from a culture of mouse fibroblast 3T3 cell line, said clonal cell line having the characteristic of accumulating relatively large amounts of triglyceride fats while in a resting state and a suitable growth media therefrom.
Claim 3
Independent Claim
74 words
A method for isolating and establishing a triglyceride-accumulating clonal cell line from a fibroblast parent cell culture comprising: a. separating a portion from said parent culture which is rich in triglyceride-accumulating cells; b. transferring said portion to a culture medium; c. growing a new culture from said transferred portion; d. repeating steps (a) through (c) until a separate colony of triglyceride-accumulating clonal cells is established; and, e. separating said colony in a pure state.
Claim 4
Independent Claim
74 words
A method for isolating and establishing a triglyceride-accumulating clonal cell line from a fibroblast parent cell culture comprising: a. separating a portion from said parent culture which is rich in triglyceride-accumulating cells; b. transferring said portion to a culture medium; c. growing a new culture from said transferred portion; d. repeating steps (a) through (c) until a separate colony of triglyceride-accumulating clonal cells is established; and, e. separating said colony in a pure state.
Claim 5
Dependent Claim
12 words
A cell culture comprising isolated 3T3-L1 cells in a suitable medium therefor.
Claim 6
Dependent Claim
12 words
A cell culture comprising isolated 3T3-L1 cells in a suitable medium therefor.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-18 | 1980-07-19 | 1981-01-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-18 | 1984-07-19 | 1985-01-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-18 | 1988-07-19 | 1989-01-18 | $7700.00 | Window Closed / Lapsed |