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US3998719A Expired / Lapsed US ⏱️ 0.0 years remaining

Isotachophoretic columns

Assignee: Czech Academy of Sciences
•
Filing: 1975-08-21
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Granted: 1976-12-21
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Expires: 1995-08-21
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Abstract

Abstract An improved isotachophoretic column for separating ions in a test sample is described. The column is formed from a main unitary insulating body, in which appropriate passages are formed to define (a) a pair of chambers for holding leading and lagging electrolytes, respectively, (b) a capillary through which ions of the material to be analyzed are passed in the presence of the electrolytes, (c) channels for interconnecting the electrolyte-holding chambers with opposite ends of the capillary, and (d) a channel for injecting the test sample into one end of the capillary. The capillary has a planar profile, with at least one of its parallel boundary walls defined by one region of the unitary body. The other boundary wall of the capillary is either formed from a second region of the unitary body itself, or by a surface of a separate insulating body that is attached to the main body. The successive ions flowing through the capillary are picked up by a suitable detector extending through the main body and into the capillary at a point intermediate its ends, and the resulting signals from the detector are recorded on an isotachophoregram.

Inventors

  • 👤 Mirko Deml
  • 👤 Petr Bocek
  • 👤 Jaroslav Janak

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-05 21:20
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 175 words
In an isotachophoretic column for separating ions in a test sample to be analyzed, comprising first and second spaced, electrolyte-receiving chambers each having an electrode associated therewith, an elongated capillary, first means for selectively interconnecting the first electrode chamber to one end of the capillary, second means for selectively interconnecting the second electrode chamber to the other end of the capillary, means for injecting a test sample to be analyzed into the capillary, means for applying a voltage across the electrodes of the first and second electrode chambers to establish a flow of ions of the test sample through the capillary, and means for detecting the ionic components in such flow, the improvement wherein the column includes a first unitary insulating body, in which the first and second electrode chambers and the first and second interconnecting means are defined by spaced portions of the first body, and wherein the capillary has first and second planar, parallel boundary walls, the first boundary wall of the capillary being defined by a first region of the first body.
Claim 2
Dependent Claim 175 words
In an isotachophoretic column for separating ions in a test sample to be analyzed, comprising first and second spaced, electrolyte-receiving chambers each having an electrode associated therewith, an elongated capillary, first means for selectively interconnecting the first electrode chamber to one end of the capillary, second means for selectively interconnecting the second electrode chamber to the other end of the capillary, means for injecting a test sample to be analyzed into the capillary, means for applying a voltage across the electrodes of the first and second electrode chambers to establish a flow of ions of the test sample through the capillary, and means for detecting the ionic components in such flow, the improvement wherein the column includes a first unitary insulating body, in which the first and second electrode chambers and the first and second interconnecting means are defined by spaced portions of the first body, and wherein the capillary has first and second planar, parallel boundary walls, the first boundary wall of the capillary being defined by a first region of the first body.
Claim 3
Dependent Claim 35 words
A column as defined in claim 1, further comprising a second insulating body having a planar surface cooperable with the first region of the first body to form the second boundary wall of the capillary.
Claim 4
Dependent Claim 26 words
A column as defined in claim 1, in which the second boundary wall of the capillary is defined by a second region of the first body.
Claim 5
Dependent Claim 31 words
A column as defined in claim 1, in which the detecting means comprises means extending through the first body and communicating electrically with the interior of the capillary intermediate its ends.
Claim 6
Dependent Claim 38 words
A column as defined in claim 1, in which the first interconnecting means comprises means defining a damping chamber in the interior of the first body intermediate the first electrode chamber and the one end of the capillary.
Claim 7
Dependent Claim 35 words
A column as defined in claim 1, in which the injecting means comprises means defining a passage extending within the first body between the second interconnecting means and an exterior surface of the first body.

References Cited (Backward Citations)

US3649499A Cited
US3705845A Cited
US3869365A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1979-12-21 1980-06-21 1980-12-21 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1983-12-21 1984-06-21 1984-12-21 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1987-12-21 1988-06-21 1988-12-21 $7700.00 Window Closed / Lapsed

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