US3998697A
Expired / Lapsed
US
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Process for preparing 2-keto-L-gulonic acid
Assignee: Shionogi and Co Ltd
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Filing: 1975-04-21
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Granted: 1976-12-21
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Expires: 1995-04-21
Abstract
Abstract 2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on or mixed contacting with a medium containing D-glucose, employing at least two kind of microorganisms; 2,5-diketo-D-gluconic acid producing strains which belong to the genera of Acetobacter, Acetomonas and Gluconobacter and strains capable of converting the 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid which belong to the general of Brevibacterium and Corynebacterium. Both the incubation of the microorganisms in a medium containing D-glucose and the direct contact of any products obtained from the cells of the microorganisms with the substrate may be used in the disclosed process. By-production of the undesired optical isomer, 2-keto-D-gluconic acid, of the intended product is effectively prevented by employing the mixed culturing or contacting because of the presence of the 2,5-diketo-D-gluconic acid producing strain or any products thereof in the medium during at least part of the entire process.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Hiroyoshi Tani
- 👤 Bunji Kageyama
- 👤 Kobee Kobayashi
- 👤 Tahiko Honjo
- 👤 Shigeo Yagi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:40
CPC Classifications
C; C07; C07H; C07H7/00; C07H7/02; C07H7/027; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/823; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 51
Claim 1
Independent Claim
158 words
A method for producing 2-keto-L-gulconic acid or a salt thereof which comprises cultivating a mixed culture of (1) a microorganism strain capable of producing 2,5-diketo-D-gluconic acid from D-glucose which belongs to the genus Acetobacter, Acetomonas or Gluconobacter or an aqueous medium containing viable cells of said microorganism having the enzymatic activity to produce 2,5-diketo-D-gluconic acid from D-glucose, and (2) a microorganism strain capable of converting 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid which belongs to the genus Brevibacterium or Corynebacterium or an aqueous medium containing viable cells of said microorganisms having the enzymatic activity to convert 2,5-diketo-D-gluconic acid into 2-keto-L-gluconic acid, in a medium containing D-glucose whereby a mixed culturing is conducted wherein both of said microorganisms or viable cells thereof are present together in the medium during at least part of the entire cultivation in a condition sufficient to produce and accumulate 2-keto-L-gulonic acid or a salt thereof in the resulting culture liquor, and recovering the 2-keto-L-gulonic acid therefrom.
Claim 2
Independent Claim
158 words
A method for producing 2-keto-L-gulconic acid or a salt thereof which comprises cultivating a mixed culture of (1) a microorganism strain capable of producing 2,5-diketo-D-gluconic acid from D-glucose which belongs to the genus Acetobacter, Acetomonas or Gluconobacter or an aqueous medium containing viable cells of said microorganism having the enzymatic activity to produce 2,5-diketo-D-gluconic acid from D-glucose, and (2) a microorganism strain capable of converting 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid which belongs to the genus Brevibacterium or Corynebacterium or an aqueous medium containing viable cells of said microorganisms having the enzymatic activity to convert 2,5-diketo-D-gluconic acid into 2-keto-L-gluconic acid, in a medium containing D-glucose whereby a mixed culturing is conducted wherein both of said microorganisms or viable cells thereof are present together in the medium during at least part of the entire cultivation in a condition sufficient to produce and accumulate 2-keto-L-gulonic acid or a salt thereof in the resulting culture liquor, and recovering the 2-keto-L-gulonic acid therefrom.
Claim 3
Dependent Claim
88 words
A method as claimed in claim 1, wherein (1) the microorganisms capable of producing 2,5-diketo-D-gluconic acid from D-glucose and (2) the microorganisms capable of converting the 2,5-diketo-D-gluconic acid into 2-keto-L-gulconic acid are incubated in the medium containing D-glucose such that both microorganisms coexist and grow together therein during at least the latter part of the cultivation, whereby 2-Keto-D-gluconic acid produced by the latter microorganism (2) is utilized by the former microorganisms (1) to produce 2,5-diketo-D-gluconic acid which is subsequently converted into 2-keto-L-gulonic acid by the latter microorganism (2).
Claim 4
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Acetobacter and the latter microorganisms strain belongs to the genus of Brevibacterium.
Claim 5
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Acetomonas and the latter microorganism strain belongs to the genus of Brevibacterium.
Claim 6
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Gluconobacter and the latter microorganism strain belongs to the genus of Brevibacterium.
Claim 7
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Acetobacter and the latter microorganism strain belongs to the genus of Corynebacterium.
Claim 8
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Acetomonas and the latter microorganism strain belongs to the genus of Corynebacterium.
Claim 9
Dependent Claim
29 words
A method as claimed in claim 2, wherein the former microorganism strain belongs to the genus of Gluconobacter and the latter microorganism strain belongs to the genus of Corynebacterium.
Claim 10
Dependent Claim
22 words
A method as claimed in claim 3, wherein the former microorganism strain belongs to the species which includes Acetobacter melanogenum IFO 3293.
Claim 11
Dependent Claim
22 words
A method as claimed in claim 6, wherein the former microorganism strain belongs to the species which includes Acetobacter melanogenum IFO 3293.
Claim 12
Dependent Claim
22 words
A method as claimed in claim 4, wherein the former microorganism strain belongs to the species which includes Acetomonas albosesamae ATCC 21998.
Claim 13
Dependent Claim
22 words
A method as claimed in claim 7, wherein the former microorganism strain belongs to the species which includes Acetomonas albosesamae ATCC 21998.
Claim 14
Dependent Claim
22 words
A method as claimed in claim 5, wherein the former microorganism strain belongs to the species which includes Gluconobacter rubiginosus IFO 3244.
Claim 15
Dependent Claim
22 words
A method as claimed in claim 8, wherein the former microorganism strain belongs to the species which includes Gluconobacter rubiginosus IFO 3244.
Claim 16
Dependent Claim
23 words
A method as claimed in claim 3, wherein the latter microorganism strain belongs to the species which includes Brevibacterium ketosoreductum ASM-1005, ATCC 21914.
Claim 17
Dependent Claim
23 words
A method as claimed in claim 4, wherein the latter microorganism strain belongs to the species which includes Brevibacterium ketosoreductum ASM-1005, ATCC 21914.
Claim 18
Dependent Claim
23 words
A method as claimed in claim 5, wherein the latter microorganism strain belongs to the species which includes Brevibacterium ketosoreductum ASM-1005, ATCC 21914.
Claim 19
Dependent Claim
23 words
A method as claimed in claim 3, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-856-4, ATCC 31083.
Claim 20
Dependent Claim
23 words
A method as claimed in claim 4, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-856-4, ATCC 31083.
Claim 21
Dependent Claim
23 words
A method as claimed in claim 5, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-856-4, ATCC 31083.
Claim 22
Dependent Claim
23 words
A method as claimed in claim 3, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-3356-31, ATCC 31082.
Claim 23
Dependent Claim
23 words
A method as claimed in claim 4, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-3356-31, ATCC 31082.
Claim 24
Dependent Claim
23 words
A method as claimed in claim 5, wherein the latter microorganism strain belongs to the species which includes Brevibacterium sp. ASM-3356-31, ATCC 31082.
Claim 25
Dependent Claim
22 words
A method as claimed in claim 3, wherein the latter microorganism strain belongs to the species which includes Brevibacterium testaceum IFO 12675.
Claim 26
Dependent Claim
22 words
A method as claimed in claim 4, wheren the latter microorganism strain belongs to the species which includes Brevibacterium testaceum IFO 12675.
Claim 27
Dependent Claim
22 words
A method as claimed in claim 5, wherein the latter microorganism strain belongs to the species which includes Brevibacterium testaceum IFO 12675.
Claim 28
Dependent Claim
23 words
A method as claimed in claim 6, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-3311-6, ATCC 31081.
Claim 29
Dependent Claim
23 words
A method as claimed in claim 7, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-3311-6, ATCC 31081.
Claim 30
Dependent Claim
23 words
A method as claimed in claim 8, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-3311-6, ATCC 31081.
Claim 31
Dependent Claim
23 words
A method as claimed in claim 6, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-20A-77, ATCC 31090.
Claim 32
Dependent Claim
23 words
A method as claimed in claim 7, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-20A-77, ATCC 31090.
Claim 33
Dependent Claim
23 words
A method as claimed in claim 8, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-20A-77, ATCC 31090.
Claim 34
Dependent Claim
23 words
A method as claimed in claim 6, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-T-13, ATCC 31089.
Claim 35
Dependent Claim
23 words
A method as claimed in claim 7, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-T-13, ATCC 31089.
Claim 36
Dependent Claim
23 words
A method as claimed in claim 8, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-T-13, ATCC 31089.
Claim 37
Dependent Claim
23 words
A method as claimed in claim 6, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-K-106, ATCC 31088.
Claim 38
Dependent Claim
23 words
A method as claimed in claim 7, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-K-106, ATCC 31088.
Claim 39
Dependent Claim
23 words
A method as claimed in claim 8, wherein the latter microorganism strain belongs to the species which includes Corynebacterium sp. ASM-K-106, ATCC 31088.
Claim 40
Dependent Claim
35 words
A method as claimed in claim 1, wherein at least one aqueous medium containing said viable cells is employed in the medium containing D-glucose in lieu of both or either one of said microorganism strains.
Claim 41
Dependent Claim
27 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Acetobacter melanogenum IFO 3293.
Claim 42
Dependent Claim
27 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Acetomonas albosesamae ATCC 21998.
Claim 43
Dependent Claim
27 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Gluconobacter rubiginosus IFO 3244.
Claim 44
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Brevibacterium ketosoreductum ASM-1005, ATCC 21914.
Claim 45
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Brevibacterium sp. ASM-856-4, ATCC 31083.
Claim 46
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Brevibacterium sp. ASM-3356-31, ATCC 31082.
Claim 47
Dependent Claim
27 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Brevibacterium testaceum IFO 12675.
Claim 48
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Corynebacterium sp. ASM-3311-6, ATCC 31081.
Claim 49
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Corynebacterium sp. ASM-20A-77, ATCC 31090.
Claim 50
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Corynebacterium sp. ASM-T-13, ATCC 31089.
Claim 51
Dependent Claim
28 words
A method as claimed in claim 39, wherein said viable cells are derived from the microorganism strain which belongs to the species including Corynebacterium sp. ASM-K-106, ATCC 31088.
References Cited (Backward Citations)
US2948659A
Cited
US3907639A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-12-21 | 1980-06-21 | 1980-12-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-12-21 | 1984-06-21 | 1984-12-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-12-21 | 1988-06-21 | 1988-12-21 | $7700.00 | Window Closed / Lapsed |