US3997657A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dry slide reagent employed in immunofluorescent test for detection of human antinuclear factor
Assignee: IL Holding Spa
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Filing: 1975-12-03
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Granted: 1976-12-14
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Expires: 1995-12-03
Abstract
Abstract A dry diagnostic reagent composed of specially prepared, fixed and stabilized fetal calf thymocytes on microscope slides ready for use in an immunofluorescent test for the detection of human anti-nuclear factor in human serum or plasma in the diagnosis of systemic lupus erythematosus and other autoimmune diseases.
Inventors
- 👤 Beatrice Frances Dziobkowski
- 👤 Gerald Earl Stiles
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 07:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/10; G01N2800/101; G01N2800/104; Y; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811; Y10S530/00; Y10S530/868
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
134 words
A method for preparing a dry diagnostic reagent fixed on a microscope slide for the detection of human antinuclear factor in human serum or plasma in an immunofluorescent test for the diagnosis of systemic lupus erythematosus and other autoimmune diseases which comprises: a. sieving only fetal calf thymus to obtain dispersed thymocyte cells therefrom; b. suspending said dispersed thymocyte cells in isotonic buffered saline solution to a cell count of about 1.0-1.5 × 10 6 cells/ml.; c. placing at least one portion of about 0.05 ml. of said dispersed thymocyte cell suspension on a microscopic slide; d. drying said amount of said dispersed thymocyte cell suspension on said slide; e. fixing said dried cell suspension with modified Carnoy's solution; f. washing said fixed suspension with water; and g. drying said fixed and washed suspension.
Claim 2
Independent Claim
134 words
A method for preparing a dry diagnostic reagent fixed on a microscope slide for the detection of human antinuclear factor in human serum or plasma in an immunofluorescent test for the diagnosis of systemic lupus erythematosus and other autoimmune diseases which comprises: a. sieving only fetal calf thymus to obtain dispersed thymocyte cells therefrom; b. suspending said dispersed thymocyte cells in isotonic buffered saline solution to a cell count of about 1.0-1.5 × 10 6 cells/ml.; c. placing at least one portion of about 0.05 ml. of said dispersed thymocyte cell suspension on a microscopic slide; d. drying said amount of said dispersed thymocyte cell suspension on said slide; e. fixing said dried cell suspension with modified Carnoy's solution; f. washing said fixed suspension with water; and g. drying said fixed and washed suspension.
Claim 3
Dependent Claim
24 words
The method of claim 1 including the additional step of placing said dried, fixed and washed slide in an air tight container with dessicant.
Claim 4
Dependent Claim
48 words
A dry diagnostic reagent fixed on a microscope slide for the detection of human antinuclear factor in human serum or plasma in an immunofluorescent test for the diagnosis of systemic lupus erythematous and other autoimmune diseases comprising the dry diagnostic reagent prepared by the method of claim 1.
Claim 5
Dependent Claim
56 words
In a method of detecting human antinuclear factor in a test sample for the diagnosis of systemic lupus erythematosus and other autoimmune diseases, said method comprising an immunofluorescent slide test employing an antigen reagent dried on a slide, the improvement comprising employing as said reagent the dry diagnostic reagent prepared by the method of claim 1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-12-14 | 1980-06-14 | 1980-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-12-14 | 1984-06-14 | 1984-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-12-14 | 1988-06-14 | 1988-12-14 | $7700.00 | Window Closed / Lapsed |