US3988206A
Expired / Lapsed
US
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Heat stable cyclodextrin glycosyltransferase
Assignee: Hayashibara Seibutsu Kagaku Kenkyujo KK
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Filing: 1974-09-23
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Granted: 1976-10-26
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Expires: 1994-09-23
Abstract
Abstract A cyclodextrin glycosyltransferase having high heat stability is obtained from the cell-free broth of a culture of Bacillus stearothermophilus. The cyclodextrin glycosyltransferase gives higher yields of cyclodextrin from starch and higher transfer ratios of glucose in starch or partial starch hydrolyzate to sucrose than known cyclodextrin glycosyltransferases.
Inventors
- 👤 Makoto Shiosaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:56
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1048; C12N9/1051; C12N9/1074; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
47 words
A cyclodextrin glycosyltransferase capable of converting starch to cyclodextrin and of transferring glucose from starch to sucrose, said cyclodextrin glycosyltransferase maintaining substantially 100% activity for 15 minutes at 65° C in an aqueous M/50 solution of Tris-HCl buffer at pH 7.0 in the absence of calcium chloride.
Claim 2
Dependent Claim
47 words
A cyclodextrin glycosyltransferase capable of converting starch to cyclodextrin and of transferring glucose from starch to sucrose, said cyclodextrin glycosyltransferase maintaining substantially 100% activity for 15 minutes at 65° C in an aqueous M/50 solution of Tris-HCl buffer at pH 7.0 in the absence of calcium chloride.
Claim 3
Dependent Claim
19 words
A cyclodextrin glycosyltransferase as set forth in claim 1 which is an extracellular product of the microorganism Bacillus stearothermophilus.
Claim 4
Dependent Claim
42 words
A cyclodextrin glycosyltransferase as set forth in claim 1, said glycosyltransferase maintaining substantially 100% activity for 15 minutes at 70° C in an aqueous M/50 solution of Tris-HCl buffer at pH 7.0 in the presence of 10 - 2 mole calcium chloride.
Claim 5
Dependent Claim
26 words
A cyclodextrin glycosyltransferase as set forth in claim 2, whrein said microorganism is Bacillus stearothermophilus FERM-P 2217, 2218, 2219, 2220, 2221, 2222, 2223, 2224 and 2225.
References Cited (Backward Citations)
US3697378A
Cited
US3812011A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-26 | 1980-04-26 | 1980-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-26 | 1984-04-26 | 1984-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-26 | 1988-04-26 | 1988-10-26 | $7700.00 | Window Closed / Lapsed |