US3986931A
Expired / Lapsed
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γ-GLUTAMYL-4-NITROANILIDE COMPOUNDS AND THEIR USE IN DETERMINING γ-GLUTAMYL TRANSPEPTIDASE
Assignee: Roche Diagnostics GmbH
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Filing: 1975-08-04
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Granted: 1976-10-19
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Expires: 1995-08-04
Abstract
Abstract γ-Glutamyl-4-nitroanilide compounds of the formula ##SPC1## Wherein R is a γ-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine γ-glutamyltranspeptidase by contacting a sample suspected of containing γ-glutamyltranspeptidase with said compound and glycyl-glycine and determining the liberation of the resulting p-nitroaniline compound as a measure of the γ-glutamyltranspeptidase activity initially present in the sample.
Inventors
- 👤 Erich Bernt
- 👤 Wolfgang Gruber
- 👤 Erich Haid
- 👤 Fritz Stahler
- 👤 August Wilhelm Wahlefeld
- 👤 Gunter Weimann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:50
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
97 words
Method of determining the γ-glutamyltranspeptidase of a content of a sample suspected of containing same, which method comprises contacting such sample with a γ-glutamyl-4-nitroanilide compound of the formula ##SPC3## wherein R is a γ-glutamyl radical and X is a carboxyl group or homologs thereof containing up to 6 carbon atoms, a sulfonic acid group, a thiosulfonyl group, a sulfhydryl group, or a phosphatyl group; and alkali, alkaline earth, ammonium and amine salts thereof and a glycylglycine and measuring the liberation of the corresponding p-nitroaniline compound as a measure of the γ-glutamyltranspeptidase activity initially present in the sample.
Claim 2
Dependent Claim
97 words
Method of determining the γ-glutamyltranspeptidase of a content of a sample suspected of containing same, which method comprises contacting such sample with a γ-glutamyl-4-nitroanilide compound of the formula ##SPC3## wherein R is a γ-glutamyl radical and X is a carboxyl group or homologs thereof containing up to 6 carbon atoms, a sulfonic acid group, a thiosulfonyl group, a sulfhydryl group, or a phosphatyl group; and alkali, alkaline earth, ammonium and amine salts thereof and a glycylglycine and measuring the liberation of the corresponding p-nitroaniline compound as a measure of the γ-glutamyltranspeptidase activity initially present in the sample.
Claim 3
Dependent Claim
16 words
Method as claimed in claim 1, wherein said liberation of the p-nitroaniline compound is measured optically.
Claim 4
Dependent Claim
23 words
Method as claimed in claim 1, wherein said γ-glutamyl-3-nitroanilide compound is in its ammonium, alkali metal, alkaline earth metal, or amine salt form.
Claim 5
Dependent Claim
15 words
Method as claimed in claim 1 wherein X in the formula is a carboxyl group.
Claim 6
Dependent Claim
16 words
Method as claimed in claim 1 wherein X in the formula is a sulfonic acid group.
Claim 7
Dependent Claim
20 words
Method as claimed in claim 1 wherein X in the formula is carboxyl-alkyline group of up to 6 carbon atoms.
Claim 8
Dependent Claim
15 words
Method as claimed in claim 1 wherein X in the formula is an acetyl group.
Claim 9
Dependent Claim
17 words
Method as claimed in claim 1 wherein X in the formula is a thiosulfonyl or sulfhydryl group.
Claim 10
Dependent Claim
15 words
Method as claimed in claim 1 wherein X in the formula is a phosphatyl group.
Claim 11
Dependent Claim
15 words
Method as claimed in claim 1 wherein said compound is in its ammonium salt form.
Claim 12
Dependent Claim
16 words
Method as claimed in claim 1 wherein said compound is in its alkali metal salt form.
Claim 13
Dependent Claim
17 words
Method as claimed in claim 1 wherein said compound is in its alkaline earth metal salt form.
Claim 14
Dependent Claim
15 words
Method as claimed in claim 1 wherein said compound is in its amine salt form.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-19 | 1980-04-19 | 1980-10-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-19 | 1984-04-19 | 1984-10-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-19 | 1988-04-19 | 1988-10-19 | $7700.00 | Window Closed / Lapsed |