US3985690A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme separation
Assignee: Individual
•
Filing: 1974-05-13
•
Granted: 1976-10-12
•
Expires: 1994-05-13
Abstract
Abstract A reactive matrix comprises a co-enzyme chemically attached to a water insoluble organic polymeric support material. The reactive matrix may be used in a process for the separation of mixtures containing a plurality of enzymes.
Inventors
- 👤 Peter Duncan Goodearl Dean
- 👤 Christopher Robin Lowe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 22:43
CPC Classifications
C; C12; C12N; C12N11/00; C08; C08L; C08L89/00; C12N9/00; C12N9/0004; C12N9/0012; C12N9/0036
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
82 words
A reactive matrix consisting essentially of a co-enzyme chemically attached to a water-insoluble organic polymeric support material in which the co-enzyme is nicotinamide adenine dinucleotide, said reactive matrix being free from chemically attached enzymes, said water-insoluble organic polymeric support material containing said co-enzyme chemically attached thereto being capable of attracting enzymes in affinity chromatography which are attracted to said coenzyme, thereby making it possible to separate enzymes from other components of a liquid medium containing said enzymes as well as other enzymes.
Claim 2
Dependent Claim
82 words
A reactive matrix consisting essentially of a co-enzyme chemically attached to a water-insoluble organic polymeric support material in which the co-enzyme is nicotinamide adenine dinucleotide, said reactive matrix being free from chemically attached enzymes, said water-insoluble organic polymeric support material containing said co-enzyme chemically attached thereto being capable of attracting enzymes in affinity chromatography which are attracted to said coenzyme, thereby making it possible to separate enzymes from other components of a liquid medium containing said enzymes as well as other enzymes.
Claim 3
Dependent Claim
15 words
A matrix according to claim 1, in which the organic polymeric support material is hydrophilic.
Claim 4
Dependent Claim
30 words
A matrix according to claim 1, in which the organic polymeric support material is in the form of a permeable material which is adapted to make up a packed column.
Claim 5
Dependent Claim
30 words
A matrix according to claim 1, in which the organic polymeric support material is a polymer containing a plurality of vicinal diol groups which have been reacted with cyanogen bromide.
Claim 6
Dependent Claim
45 words
A matrix according to claim 1, in which the co-enzyme is linked to the backbone of the polymer chain of the organic polymeric support material by an intermediate carbon chain containing from 2 to 20 carbon atoms or by an oligopeptide or a polyaromatic system.
Claim 7
Dependent Claim
15 words
A matrix according to claim 1, in which the organic polymeric support material is polyacrylamide.
Claim 8
Dependent Claim
52 words
A matrix according to claim 1, in which the organic polymeric support material is selected from the group consisting of polyacrylamide, cellulose acetate, a starch, a dextran, a protein, polyvinyl alcohol, nylon, a polyester, chloromethylated polystyrenes and polymers containing a plurality of vicinal diol groups which have been reacted with cyanogen bromide.
Claim 9
Dependent Claim
12 words
A matrix according to claim 4, in which said polymer is cellulose.
References Cited (Backward Citations)
DE1959169A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-12 | 1980-04-12 | 1980-10-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-12 | 1984-04-12 | 1984-10-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-12 | 1988-04-12 | 1988-10-12 | $7700.00 | Window Closed / Lapsed |