Process for the preparation of galactose and beverages based on galactose from solutions containing lactose

Claim 2

A process for the preparation of a solution containing galactose from a solution containing lactose comprising: a. inoculating said solution containing lactose with a culture of a living mutant, non-pathogenic, prototrophic yeast having β-galactosidase and gal - character selected from the group consisting of ascomycetes and basidiomycetes; b. fermenting said solution to produce a galactose containing solution; and c. recovering said galactose containing solution.

Claim 3

The process of claim 1, wherein said yeast is a diploid.

Claim 4

The process of claim 1, wherein said yeast is obtained by mutation and selection.

Claim 5

The process of claim 1, wherein said solution containing lactose is selected from the group consisting of lactoserum, whole milk, semi-skimmed milk and skimmed milk.

Claim 6

The process of claim 1, wherein the solution containing lactose is a lactoserum.

Claim 7

A process for the preparation of a solution containing galactose, from a solution containing lactose, comprising: a. innoculating said solution containing lactose with a culture of a living mutant, non-pathogenic, prototrophic microorganism having β-galactosidase and a gal - character and selected from the group consisting of bacteria selected from Enterobacteriaceae and Lactobacteriaceae and yeast selected from Debaryomyces cantarellii Debaryomyces castellii Debaryomyces hansenii Debaryomyces morana Debaryomyces tamarii Hansenula capsulata Kluyveromyces aestuarii Kluyveromyces bulgarious Kluyveromyces cicerisporus Kluyveromyces fragilis Kluyveromyces lactis kluyveromyces wikerhamii Lipomyces lipofer Lipomyces starkeyi Pichia farinosa Pichia polymorpha Pichia pseudopolymorpha Pichia scolyti Pichia stipitis Schwanniomyces castellii Wingea robertsii Leucosporium frigidum Leucosporidium scottii Bullera alba Bullera strigea Sporobolomyces singularis Brettanomyces anomalus Brettanomyces claussenii Brettanomyces curtersii Candida aaseri Candida aquatica Candida blankii Candida curvata Candida glaebosa Candida humicola Candida intermedia Candida kefyr Candida macedoniensis Candida muscorum Candida pseudotropicalis Candida salmanticensis Candida shehatae Candida tenuis Cryptococcus albidus Cryptococcus ater Cryptococcus dimennae Cryptococcus flavus Cryptococcus gastricus Cryptococcus hungaricus Cryptococcus infirmo-miniatus Cryptococcus laurentii Cryptococcus macerans Cryptococcus malibiosum Cryptococcus terreus Rhodotorula aurantiaca Rhodotorula lactosa Rhodotorula marina Rhodoturula minuta Sterigmatomyces halophilus Torulopsis candida Torulopsis ingeniosa Torulopsis versatilis Trichosporum cutaneum Trichosporum inkin Trichosporum pullulans; b. fermenting said solution to produce a galactose containing solution; and c. recovering said galactose containing solution.

Claim 8

A process for the preparation of a solution containing galactose, from a solution containing lactose, comprising: a. innoculating said solution containing lactose with a culture of a living mutant, non-pathogenic, prototrophic microorganism having β-galactosidase and a gal - character and selected from the group consisting of bacteria selected from Enterobacteriaceae and Lactobacteriaceae and yeast selected from Debaryomyces cantarellii Debaryomyces castellii Debaryomyces hansenii Debaryomyces morana Debaryomyces tamarii Hansenula capsulata Kluyveromyces aestuarii Kluyveromyces bulgarious Kluyveromyces cicerisporus Kluyveromyces fragilis Kluyveromyces lactis kluyveromyces wikerhamii Lipomyces lipofer Lipomyces starkeyi Pichia farinosa Pichia polymorpha Pichia pseudopolymorpha Pichia scolyti Pichia stipitis Schwanniomyces castellii Wingea robertsii Leucosporium frigidum Leucosporidium scottii Bullera alba Bullera strigea Sporobolomyces singularis Brettanomyces anomalus Brettanomyces claussenii Brettanomyces curtersii Candida aaseri Candida aquatica Candida blankii Candida curvata Candida glaebosa Candida humicola Candida intermedia Candida kefyr Candida macedoniensis Candida muscorum Candida pseudotropicalis Candida salmanticensis Candida shehatae Candida tenuis Cryptococcus albidus Cryptococcus ater Cryptococcus dimennae Cryptococcus flavus Cryptococcus gastricus Cryptococcus hungaricus Cryptococcus infirmo-miniatus Cryptococcus laurentii Cryptococcus macerans Cryptococcus malibiosum Cryptococcus terreus Rhodotorula aurantiaca Rhodotorula lactosa Rhodotorula marina Rhodoturula minuta Sterigmatomyces halophilus Torulopsis candida Torulopsis ingeniosa Torulopsis versatilis Trichosporum cutaneum Trichosporum inkin Trichosporum pullulans; b. fermenting said solution to produce a galactose containing solution; and c. recovering said galactose containing solution.

Claim 9

The process of claim 6 wherein said bacteria is an Escherichia coli.

Claim 10

The process of claim 7 wherein said bacteria is an Escherichia coli, deposited under the number CBS 6051 B at the Centraal Bureau voor Schimmelcultures in Delft, Holland.

Claim 11

The process of claim 6, wherein said bacteria is a bacteria obtained from a mutant non-pathogenic auxotrophic bacteria possessing a β-galactosidase and a gal - character which has been reversed for its various auxotrophs.

Claim 12

The process of claim 6 wherein said yeast is a diploid.

Claim 13

The process of claim 6 wherein said yeast is selected from the Kluyveromyces fragilis strains deposited at the Centraal Bureau voor Schimmelcultures in Delft, Holland, under the numbers CBS 6498 and 6499.

Claim 14

The process of claim 6 wherein said yeast is obtained by mutation and selection.

Claim 15

The process of claim 6 wherein said solution containing lactose is selected from the group consisting of lactoserum, whole milk, semi-skimmed milk and skimmed milk.

Claim 16

The process of claim 13 wherein the solution containing lactose is a lactoserum.