US3979669A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Particle analyzing system
Assignee: Coulter Electronics Inc
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Filing: 1975-06-24
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Granted: 1976-09-07
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Expires: 1995-06-24
Abstract
Abstract A particle analyzing system of the type having an aperture retaining member for obtaining signals from microscopic particles suspended in a fluid which passes through a scanning aperture. The aperture opens on one side thereof to the fluid suspension and on the opposite side to a passageway in the aperture retaining member; the passageway is connected at an entrance end thereof to a source of clean electrolyte and at an exit end thereof to a waste collecting container or isolator and a vacuum source. A vacuum from the source is applied to the collecting container to cause the clean electrolyte to be drawn through the passageway and wash or sweep behind the aperture simultaneously with passage of the suspension through the aperture. A sealed sweep flow isolator drip chamber or tank is interposed in the fluid conduits between the source of clean electrolyte and the passageway with a respective restriction in the fluid conduit on either side of the tank to enable filling of the tank with clean electrolyte to a predetermined desired level below the sealed top of the tank thereby to prevent electrical connection between the electrolyte supply and the passageway. The waste isolator is provided with a second aperture retaining member disposed in the wall thereof. Sample which has been sensed in the scanning aperture and diluted with the sweep flow clean electrolyte mixed therewith in the passageway is accumulated in the isolator to be tested with low coincidence correction required by passing the same through the second aperture into a secondary isolator. Alternatively, diluted sample present in the fluid conduit between the exit end of the passageway and the waste isolator may itself be forced back through the scanning aperture by applying pressure to the waste isolator; the diluted sample thereby is tested with low coincidence correction required.
Inventors
- 👤 Thomas J. Godin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:56
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/1031; G01N15/12; G01N1/00; G01N1/28; G01N1/38
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
230 words
A particle-free electrolyte supply system for particle measuring apparatus in which an aperture retaining member is provided in a vessel for containing a body of particulate liquid suspension to be analyzed, the member having an aperture to permit passage of the suspension from the vessel into the member, the apparatus including a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte, an outlet chamber in fluid communication with the inlet chamber, an electrolyte isolator and first conduit for fluid communication between the isolator and inlet chamber, an electrolyte supply and second conduit for fluid communication between the supply and isolator, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container, said system comprising, a first restriction in the first conduit and a second restriction in the second conduit, whereby the fluid moving means will cause the electrolyte to pass from the supply to the isolator but the volume of electrolyte in the isolator will be established and maintained constant.
Claim 2
Dependent Claim
230 words
A particle-free electrolyte supply system for particle measuring apparatus in which an aperture retaining member is provided in a vessel for containing a body of particulate liquid suspension to be analyzed, the member having an aperture to permit passage of the suspension from the vessel into the member, the apparatus including a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte, an outlet chamber in fluid communication with the inlet chamber, an electrolyte isolator and first conduit for fluid communication between the isolator and inlet chamber, an electrolyte supply and second conduit for fluid communication between the supply and isolator, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container, said system comprising, a first restriction in the first conduit and a second restriction in the second conduit, whereby the fluid moving means will cause the electrolyte to pass from the supply to the isolator but the volume of electrolyte in the isolator will be established and maintained constant.
Claim 3
Dependent Claim
41 words
A system as claimed in claim 1 in which the isolator is a drip container and the electrolyte drips across an air gap therein to pool at the bottom thereof, but the level of electrolyte in the container is maintained constant.
Claim 4
Dependent Claim
26 words
A system as claimed in claim 1 in which the restrictions are chokes and the relationship between the first choke to the second choke is 0.012:0.016.
Claim 5
Dependent Claim
49 words
A system as claimed in claim 1 in which the electrolyte and the tested suspension form a dilution accumulated in the waste container, said waste container having means provided in a wall thereof to sense particles in the dilution electrically and thereafter to pass same to a secondary container.
Claim 6
Dependent Claim
28 words
A system as claimed in claim 4 in which said last named means include a socket in the wall and an aperture retaining fitting engaged in said socket.
Claim 7
Dependent Claim
42 words
A system as claimed in claim 1 in which the electrolyte and the analyzed suspension form a dilution accumulated in the waste container, and means associated with the waste container to return the dilution back through the aperture to analyze the dilution.
Claim 8
Dependent Claim
22 words
A system as claimed in claim 6 in which said last named means include a pressure source coupled with said waste container.
Claim 9
Dependent Claim
42 words
A system as claimed in claim 7 in which the waste container and outlet chamber are connected by a conduit defining a volume of said dilution and said volume is returned through the aperture upon application of pressure by said pressure source.
Claim 10
Dependent Claim
206 words
A particle analyzing system for analyzing microscopic particles in a suspension, the system comprising, a vessel for containing a body of the suspension, an aperture retaining member provided in the vessel, the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte into the path of the suspension to mix therewith and form a dilution, an outlet chamber in fluid communication with the inlet chamber to receive the dilution, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container to move the dilution into the waste container to be accumulated therein, the waste container having means provided in a wall thereof to analyze the dilution a second time and thereafter to convey the same to a secondary container.
Claim 11
Dependent Claim
206 words
A particle analyzing system for analyzing microscopic particles in a suspension, the system comprising, a vessel for containing a body of the suspension, an aperture retaining member provided in the vessel, the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte into the path of the suspension to mix therewith and form a dilution, an outlet chamber in fluid communication with the inlet chamber to receive the dilution, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container to move the dilution into the waste container to be accumulated therein, the waste container having means provided in a wall thereof to analyze the dilution a second time and thereafter to convey the same to a secondary container.
Claim 12
Dependent Claim
28 words
A system as claimed in claim 9 in which said last named means include a socket in the wall and an aperture retaining fitting engaged in said socket.
Claim 13
Dependent Claim
198 words
A particle analyzing system for analyzing microscopic particles in a suspension, the system comprising, a vessel for containing a body of the suspension, an aperture retaining member provided in the vessel, the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte into the path of the suspension to mix therewith and form a dilution, an outlet chamber in fluid communication with the inlet chamber to receive the dilution, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container to move the dilution into the waste container to be accumulated therein, the waste container having means associated therewith to return the dilution back through the aperture to analyze the dilution.
Claim 14
Dependent Claim
198 words
A particle analyzing system for analyzing microscopic particles in a suspension, the system comprising, a vessel for containing a body of the suspension, an aperture retaining member provided in the vessel, the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having an inlet chamber for introduction of particle free electrolyte into the path of the suspension to mix therewith and form a dilution, an outlet chamber in fluid communication with the inlet chamber to receive the dilution, a waste container in fluid communication with the outlet chamber, and fluid moving means connected to the waste container to move the dilution into the waste container to be accumulated therein, the waste container having means associated therewith to return the dilution back through the aperture to analyze the dilution.
Claim 15
Dependent Claim
22 words
A system as claimed in claim 11 in which said last named means include a pressure source coupled with said waste container.
Claim 16
Dependent Claim
42 words
A system as claimed in claim 12 in which the waste container and outlet chamber are connected by a conduit defining a volume of said dilution and said volume is returned through the aperture upon application of pressure by said pressure source.
Claim 17
Independent Claim
209 words
A method for analyzing microscopic particles in a particle analyzing device including a vessel for containing the particles in a body of suspension, an aperture retaining member provided in the vessel and the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having in inlet chamber and an outlet chamber in fluid communication with the inlet chamber, a sweep-flow container for retaining particle free electrolyte in fluid communication with the inlet chamber, and a waste container in fluid communication with the outlet chamber, said method comprising the steps of: moving the electrolyte from the sweep-flow container into the inlet chamber and simultaneously moving the suspension through the aperture to mix with the electrolyte to form a dilution, accumulating the dilution in the waste container, and returning the dilution back through the aperture to analyze the particles in the dilution.
Claim 18
Independent Claim
209 words
A method for analyzing microscopic particles in a particle analyzing device including a vessel for containing the particles in a body of suspension, an aperture retaining member provided in the vessel and the member having an aperture to permit passage of the suspension from the vessel into the member, a first electrode in the vessel and a second electrode in the member to establish an electrical field in the aperture between the vessel and the member, means including electrical leads connected to the electrodes and adapted to extend connections to a detector to respond to electrical measuring signals produced across the electrodes with passage of the particles through said aperture, the member having in inlet chamber and an outlet chamber in fluid communication with the inlet chamber, a sweep-flow container for retaining particle free electrolyte in fluid communication with the inlet chamber, and a waste container in fluid communication with the outlet chamber, said method comprising the steps of: moving the electrolyte from the sweep-flow container into the inlet chamber and simultaneously moving the suspension through the aperture to mix with the electrolyte to form a dilution, accumulating the dilution in the waste container, and returning the dilution back through the aperture to analyze the particles in the dilution.
Claim 19
Dependent Claim
23 words
A method as claimed in claim 14 in which the step of moving is performed by applying a vacuum to the waste container.
Claim 20
Dependent Claim
26 words
A method as claimed in claim 14 in which the step of returning is performed by applying pressure to the waste container from the exterior thereof.
Claim 21
Dependent Claim
41 words
A method as claimed in claim 14 in which the waste container and outlet chamber are connected by a conduit defining a volume of said dilution and the step of returning is performed by returning said volume back through the aperture.
References Cited (Backward Citations)
US3902115A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-07 | 1980-03-08 | 1980-09-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-07 | 1984-03-08 | 1984-09-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-07 | 1988-03-08 | 1988-09-07 | $7700.00 | Window Closed / Lapsed |