US3973001A
Expired / Lapsed
US
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Tissue cell stimulating blood extracts
Assignee: ICN Switzerland AG
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Filing: 1975-01-22
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Granted: 1976-08-03
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Expires: 1995-01-22
Abstract
Abstract Novel blood extracts of vertebrata, i.e. animals having a vertebral column, especially warm blooded animals, which blood extracts possess cell respiration stimulating activity and stimulate the growth of cells and to novel processes for the preparation of the said blood extracts.
Inventors
- 👤 Karl-Heinz Jaeger
- 👤 Hellmut Mittenzwei
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:40
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
126 words
A process for the preparation of a tissue cell respiration stimulating blood extract which comprises (1) recovering the fresh blood of a calf, (2) defibrinating the said blood, (3) hemolyzing the defibrinated blood by stirring the latter with distilled water until hemolysis is complete, (4) removing the solids from the hemolyzed solution, (5) subjecting the resulting solution to dialysis with a cellophane membrane against an aqueous solution selected from the group consisting of distilled water and aqueous ethanol until substantially no nitrogen migrates to the dialysate, then concentrating and adjusting the pH of the outer dialysate to about 7.0 and (6) concentrating the said dialysate to a concentration of about 10 to 60 mg of dry components per ml of solution, and removing any inactive precipitate.
Claim 2
Dependent Claim
126 words
A process for the preparation of a tissue cell respiration stimulating blood extract which comprises (1) recovering the fresh blood of a calf, (2) defibrinating the said blood, (3) hemolyzing the defibrinated blood by stirring the latter with distilled water until hemolysis is complete, (4) removing the solids from the hemolyzed solution, (5) subjecting the resulting solution to dialysis with a cellophane membrane against an aqueous solution selected from the group consisting of distilled water and aqueous ethanol until substantially no nitrogen migrates to the dialysate, then concentrating and adjusting the pH of the outer dialysate to about 7.0 and (6) concentrating the said dialysate to a concentration of about 10 to 60 mg of dry components per ml of solution, and removing any inactive precipitate.
Claim 3
Dependent Claim
22 words
The process of claim 1 in which the calf has been subjected to stress by irradiation prior to recovering the fresh blood.
Claim 4
Dependent Claim
27 words
The process of claim 1 in which the calf has been subjected to stress by injection of a hypotonic aqueous solution prior to recovering the fresh blood.
Claim 5
Dependent Claim
136 words
A process for the preparation of tissue cell respiration stimulating blood extracts which comprises (1) recovering the fresh blood of a calf, (2) defibrinating the said blood by stirring at room temperature, (3) hemolyzing the defibrinated blood by stirring the latter with about an equal volume of distilled water until hemolysis is complete, (4) removing the solids from the hemolyzed solution, (5) subjecting the resulting solution to dialysis with a cellophane membrane against an aqueous solution selected from the group consisting of distilled water and aqueous ethanol until substantially no nitrogen migrates to the dialysate, then concentrating and adjusting the pH of the outer dialysate to about 7.0 and (6) then further twice concentrating the said dialysate to a concentration of about 40 mg of dry components per ml of solution, and removing any inactive precipitate.
Claim 6
Dependent Claim
136 words
A process for the preparation of tissue cell respiration stimulating blood extracts which comprises (1) recovering the fresh blood of a calf, (2) defibrinating the said blood by stirring at room temperature, (3) hemolyzing the defibrinated blood by stirring the latter with about an equal volume of distilled water until hemolysis is complete, (4) removing the solids from the hemolyzed solution, (5) subjecting the resulting solution to dialysis with a cellophane membrane against an aqueous solution selected from the group consisting of distilled water and aqueous ethanol until substantially no nitrogen migrates to the dialysate, then concentrating and adjusting the pH of the outer dialysate to about 7.0 and (6) then further twice concentrating the said dialysate to a concentration of about 40 mg of dry components per ml of solution, and removing any inactive precipitate.
Claim 7
Dependent Claim
9 words
The product produced by the process of claim 1.
Claim 8
Dependent Claim
9 words
The product produced by the process of claim 2.
Claim 9
Dependent Claim
9 words
The product produced by the process of claim 3.
Claim 10
Dependent Claim
9 words
The product produced by the process of claim 4.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-03 | 1980-02-02 | 1980-08-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-03 | 1984-02-02 | 1984-08-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-03 | 1988-02-02 | 1988-08-03 | $7700.00 | Window Closed / Lapsed |