US3972773A
Expired / Lapsed
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Process for producing peptide
Assignee: Nitto Boseki Co Ltd
•
Filing: 1975-04-29
•
Granted: 1976-08-03
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Expires: 1995-04-29
Abstract
Abstract A peptide having the formula:
Inventors
- 👤 Yoshikazu Isowa
- 👤 Takeshi Nagasawa
- 👤 Katsumasa Kuroiwa
- 👤 Koichi Narita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:24
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/06; C07K1/00; C07K1/02; C07K5/00; C07K5/04; C07K5/06; C07K5/06008; C07K5/06017; C07K5/06034; C07K5/06043; C07K5/06086; C07K5/06095; C07K5/06104; C07K5/06113; C07K5/06139; C07K5/06147; C07K5/08; C07K5/0802; C07K5/0804; C07K5/0819; C07K5/10; C07K5/1002; C07K5/1005; C07K5/1008; C07K5/1013; C07K5/1016; C07K5/1021; C12; C12P; C12P21/00; C12P21/02; A; A61; A61K; A61K38/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
169 words
A process for producing a peptide having the formula: X-A-B-C-Y wherein A represents Ala., Gln., Asn, ω-BOC-Lys., Leu., Gly., Glu., ##EQU7## or A represents A 1 -- A 2 , wherein A 1 represents a hydrophilic amino acid residue and A 2 represents Val., Met., Leu. or Gln.; B represents Phe., Tyr., Leu., Met., Glu., Asp., Gln., Asn or Trp.; C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., Ser-OBzl., Trp. or Met.; X represents an α-amino acid protective group, an amino acid residue having an N-terminal protective group or a peptide residue having an N-terminal protective group; Y represents a carboxyl protective group, an amino acid residue having a C-terminal protective group or a peptide residue having a C-terminal protective group, which comprises reacting a peptide having the formula: X-A-B-OH with an amino acid or a peptide having the formula: H-C-Y neither of which reactants need be activated, in the presence of enzyme pepsin employing a pH of from 2 to 6 and a temperature of 20° - 50° C.
Claim 2
Dependent Claim
169 words
A process for producing a peptide having the formula: X-A-B-C-Y wherein A represents Ala., Gln., Asn, ω-BOC-Lys., Leu., Gly., Glu., ##EQU7## or A represents A 1 -- A 2 , wherein A 1 represents a hydrophilic amino acid residue and A 2 represents Val., Met., Leu. or Gln.; B represents Phe., Tyr., Leu., Met., Glu., Asp., Gln., Asn or Trp.; C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., Ser-OBzl., Trp. or Met.; X represents an α-amino acid protective group, an amino acid residue having an N-terminal protective group or a peptide residue having an N-terminal protective group; Y represents a carboxyl protective group, an amino acid residue having a C-terminal protective group or a peptide residue having a C-terminal protective group, which comprises reacting a peptide having the formula: X-A-B-OH with an amino acid or a peptide having the formula: H-C-Y neither of which reactants need be activated, in the presence of enzyme pepsin employing a pH of from 2 to 6 and a temperature of 20° - 50° C.
Claim 3
Dependent Claim
39 words
The process of claim 1 which comprises using a peptide having the formula: X-A-B-OH wherein A represents Ala., Gln., Asn., ω-BOC-Lys., Leu., or Gly.; B represents Phe., Tyr., Leu., or Met.; and X is defined as in claim 1.
Claim 4
Dependent Claim
30 words
The process of claim 1 which comprises using a peptide having the formula: X-A-B-OH wherein A represents Glu., ##EQU8##or Pro.; and B and X are defined as in claim 1.
Claim 5
Dependent Claim
47 words
The process of claim 1 which comprises using a peptide having the formula: X-A.sub.1 - A.sub.2 - B - OH wherein A 1 represents a hydrophilic amino acid residue; A 2 represents Val., Met., Leu. or Gln.; and B and X are defined as in claim 1.
Claim 6
Dependent Claim
36 words
The process of claim 1 which comprises using an amino acid or a peptide having the formula: H-C-Y wherein C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., or Ser-OBzl; and Y is defined as in claim 1.
Claim 7
Dependent Claim
26 words
The process of claim 1 wherein the reaction is performed at 20° - 50° C in a buffer solution having a pH of 2 - 6.
Claim 8
Dependent Claim
31 words
The process of claim 1 wherein the molar ratio of the peptide having the formula: X-A-B-OH to the amino acid or peptide having the formula: H-C-Y is 1:5 to about 5:1.
Claim 9
Dependent Claim
21 words
The process of claim 1 wherein the amount of pepsin is 0.4 - 400 mg per 1 mmole of starting materials.
Claim 10
Dependent Claim
18 words
The process of claim 4 wherein A 1 is Ala., Gln., Asn., ω-BOC-Lys, Leu., Gly., Glu., ##EQU9##or Pro.
References Cited (Backward Citations)
US3544426A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-03 | 1980-02-02 | 1980-08-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-03 | 1984-02-02 | 1984-08-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-03 | 1988-02-02 | 1988-08-03 | $7700.00 | Window Closed / Lapsed |