Method and apparatus for computation in a kinetic analyzer

Claim 3

The method of claim 1 and further including the step of computing curvature of the reaction rate over said observation period.

Claim 4

The method of claim 2 wherein said curvature computing step is accomplished by the steps of: a. integrating the absorbance signal in a first direction for the first quarter of said observation period; b. integrating the absorbance signal in a first direction for the second and third quarters of said observation period; c. integrating the absorbance signal in said first direction for the fourth quarter of said observation period; and d. finding the net value of said integrations, said net value being proportional to the curvature of the reaction rate over said observation period.

Claim 5

The method of claim 3 and further including the step of comparing said net value proportional to the curvature with said net value proportional to the reaction rate and providing an indication when the former differs from the latter by a predetermined percentage thereof.

Claim 6

The method of claim 4 and further including the step of checking the sign of the difference when said predetermined percentage is exceeded and providing a first indication if the difference is positive and a second indication if the difference is negative.

Claim 7

The method of claim 5 and further including the steps of changing the scale factor during each quarter of said observation period to verify a non-linear reaction rate.

Claim 8

The method of claim 1 and further including the step of converting the absorbance signal to pulses at a frequency proportional thereto and performing said integrating steps by counting said pulses in one direction for the first half of said observation period and in an opposite direction for the second half of said observation period, the net count being inherently proportional to both the average change in the absorbance signal and the reaction rate over said observation period.

Claim 9

The method of claim 7 and further including the step of computing curvature of the reaction rate over said observation period.

Claim 10

The method of claim 8 wherein said curvature computing step is accomplished by the steps of: a. integrating the absorbance signal in a first direction for the first quarter of said observation period; b. integrating the absorbance signal in the opposite direction for the second and third quarters of said observation period; c. integrating the absorbance signal in said first direction for the fourth quarter of said observation period; and d. finding the net value of said integrations, said net value being proportional to the curvature.

Claim 11

The method of claim 9 and further including the step of comparing said net value proportional to the curvature with said net value proportional to the reaction rate and providing an indication when the former differs from the latter by a predetermined percentage thereof.

Claim 12

The method of claim 10 and further including the step of checking the sign of the difference when said predetermined percentage is exceeded and providing a first indication if the difference is positive and a second indication if the difference is negative.

Claim 13

The method of claim 11, wherein said integrating steps of said curvature computing step is accomplished by the steps of: a. counting said pulses at the frequency proportional to the absorbance signal in a first direction during the first quarter of said observation period; b. counting said pulses in the opposite direction during the second and third quarters of said observation period; and c. counting said pulses in said first direction during the fourth quarter of said observation period, the net count being proportional to the curvature of the reaction rate over said observation period.

Claim 14

The method of claim 12 wherein said step of comparing said net value proportional to the curvature with said net value proportional to the reaction rate and providing an indication when the former differs from the latter by said predetermined precentage is accomplished by concurrently counting the net counts proportional to the curvature and the reaction rate towards zero with the net count of the curvature being counted at a lower frequency than the net count of the reaction rate and providing said indication if the net count of the curvature does not reach zero simultaneously with the net count of the reaction rate, said indication being a first error output when the remaining net count of the curvature is positive and being a second error output when that remaining net count is negative.

Claim 15

The method of claim 13 and further including the step of checking the level of said absorbance signal against upper and lower limits and providing third and fourth error outputs respectively if said limits are exceeded.

Claim 16

The method of claim 14 and further including the step of printing out decimal representation of the reaction rate along with an additional message that any one of said first, second, third, or fourth error signals is associated therewith.

Claim 17

The method of claim 15 and further including the step of assigning a priority to each of said error signals and printing out only the error signal of highest priority.

Claim 18

Apparatus for computing the reaction rate in a sample cell from a signal proportional to absorbance therein, comprising: a. means for establishing an observation period; b. means for integrating the absorbance signal in a first direction during the first half of said observation period; c. means for integrating the absorbance signal in the opposite direction during the second half of said observation period; and d. means for determining the difference between the two integrations, said difference being proportional to both the average change in the absorbance signal and the reaction rate in the cell over said observation period.

Claim 19

Apparatus for computing the reaction rate in a sample cell from a signal proportional to absorbance therein, comprising: a. means for establishing an observation period; b. means for integrating the absorbance signal in a first direction during the first half of said observation period; c. means for integrating the absorbance signal in the opposite direction during the second half of said observation period; and d. means for determining the difference between the two integrations, said difference being proportional to both the average change in the absorbance signal and the reaction rate in the cell over said observation period.

Claim 20

Apparatus according to claim 17 and further including means for offsetting the absorbance signal to a value near zero immediately before beginning said observation period.

Claim 21

Apparatus according to claim 17 and further including means for computing the curvature of the reaction rate over said observation period.

Claim 22

Apparatus according to claim 19 wherein said curvature computing means includes: means for integrating the absorbance signal in a first direction during the first quarter of said observation period, means for integrating the absorbance signal in the opposite direction during the second and third quarters of said observation period means for integrating the absorbance signal in said first direction during the fourth quarter of said observation period, and means for determining the net value of said integrations, said net value being proportional to the curvature.

Claim 23

Apparatus according to claim 20 and further including means for changing the scale factor during each quarter of said observation period to verify a non-linear reaction rate.

Claim 24

Apparatus according to claim 17 and further including means for comparing the absorbance signal level with pre-established maximum and minimum limits and providing first and second error outputs respectively if said limits are exceeded.

Claim 25

Apparatus according to claim 17 and further including means for generating pulses at a frequency proportional to the absorbance signal and wherein said means for integrating includes: a. an up-down rate register; b. means for coupling said pulses to the down input of said rate register during the first half of said observation period; and c. means for coupling said pulses to the up input of said register during the second half of said observation period, the net count in said register being proportional to both the average change in the absorbance signal and the reaction rate over said observation period.

Claim 26

Apparatus according to claim 23 and further including means for computing the curvature of the reaction rate over said observation period.

Claim 27

Apparatus according to claim 24 wherein said curvature computing means includes: means for integrating the absorbance signal in a first direction during the first quarter of said observation period, means for integrating the absorbance signal in the opposite direction during the second and third quarters of said observation period, means for integrating said absorbance signal in said first direction during the fourth quarter of said observation period, and means for determining the net value of said integrations, said net value being proportional to the curvature.

Claim 28

Apparatus according to claim 25 wherein said integrating means of said curvature computing means includes: a. an up-down curvature register; b. means for coupling said pulses from said generating means to the up input of said curvature register during the first quarter of said observation period; c. means for coupling said pulses to the down input of said curvature register during the second and third quarters; and d. means for coupling said pulses to the up input of said curvature register during the fourth quarter, the net count in said curvature register being proportional to the curvature.

Claim 29

The apparatus according to claim 26 and further including means for comparing the net count proportional to the curvature with a predetermined percentage of the net count proportional to the reaction rate.

Claim 30

Apparatus according to claim 27 wherein said comparing means includes: a. an output register; b. means for concurrently counting all of said registers, said rate register and said curvature register being counted toward zero and said output register being counted up, said curvature register being counted at a lower frequency than said rate register and said output register being counted at the same frequency as said rate register, said predetermined percentage being proportional to the difference in frequencies of said counting means, said curvature register having a remaining count therein after said rate register reaches zero when the curvature exceeds said predetermined percentage.

Claim 31

Apparatus according to claim 28 and further including means for indicating when the curvature exceeds said predetermined percentage.

Claim 32

Apparatus according to claim 29 wherein said indicating means includes: a. first gate means responsive when said remaining count in said curvature register is positive to provide a first error output; and b. second gate means responsive when said remaining count in said curvature register is negative to provide a second error output.

Claim 33

Apparatus according to claim 30 and further including means for comparing the absorbance signal level with pre-established maximum and minimum limits and providing third and fourth error outputs respectively when said limits are exceeded.

Claim 34

Apparatus according to claim 31 and further including means for sensing overflow conditions from the count in said output register and for providing a fifth error output therefrom.

Claim 35

Apparatus according to claim 32 wherein the count of said output register and said error outputs are coupled to a printer.

Claim 36

Apparatus according to claim 33 wherein said error outputs are coupled to said printer through means for selecting said error output of highest priority when more than one of said error outputs exist.