US3963574A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing 2-keto-L-gulonic acid
Assignee: Individual
•
Filing: 1975-03-21
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Granted: 1976-06-15
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Expires: 1995-03-21
Abstract
Abstract 2-Keto-L-gulonic acid is prepared from 2,5-diketo-D-gluconic acid through microbial conversion. The 2-keto-L-gulonic acid producing microorganism available for this microbial conversion includes strains which belong to the species of the Brevibacterium nov. sp. ASM-856-4, ATCC 31083. The incubation of the microorganism in a medium containing 2,5-diketo-D-gluconic acid as well as the direct contact of any products obtained from the microbial cells with a substrate containing said 2,5-diketo-D-gluconic acid may be used in the disclosed process.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Hiroyoshi Tani
- 👤 Bunji Kageyama
- 👤 Kobee Kobayashi
- 👤 Tahiko Honjo
- 👤 Shigeo Yagi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:34
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; contacting the 2-keto-L-gulonic acid producing microorganism strain, Brevibacterium nov. sp. ASM-856-4, ATCC 31083, or treated cells of said microorganism strain having the enzyme activity to convert 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, with 2,5-diketo-D-gluconic acid or a salt thereof, under conditions sufficient to produce 2-keto-L-gulonic acid, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resulting mixture.
Claim 2
Dependent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; contacting the 2-keto-L-gulonic acid producing microorganism strain, Brevibacterium nov. sp. ASM-856-4, ATCC 31083, or treated cells of said microorganism strain having the enzyme activity to convert 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, with 2,5-diketo-D-gluconic acid or a salt thereof, under conditions sufficient to produce 2-keto-L-gulonic acid, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resulting mixture.
Claim 3
Dependent Claim
45 words
A process as claimed in claim 1, wherein said microorganism strain is inoculated with and incubated in a medium containing 2,5-diketo-D-gluconic acid or a salt thereof to effect a fermentative action therein and recovering the 2-keto-L-gulonic acid or any salts thereof accumulated during the incubation.
Claim 4
Dependent Claim
63 words
A process as claimed in claim 1, wherein an aqueous medium containing viable cells of said microorganism strain is contacted with a substance containing 2,5-diketo-D-gluconic acid in a form utilizable by said microorganism strain or a salt of said substance under conditions sufficient to produce 2-keto-L-gulonic acid, and recovering said 2-keto-L-gulonic acid or any salts thereof produced and accumulated in the resulting mixture.
Claim 5
Dependent Claim
41 words
A process as claimed in claim 1, wherein said microorganism strain or treated cells thereof are contacted with said 2,5-diketo-D-gluconic acid or salt thereof at a temperature of about 20° to 35°C. and at a pH of about 4 to 9.
Claim 6
Dependent Claim
67 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; cultivating the microorganism Brevibacterium nov. sp. ASM-856-4, ATCC 31083, or treated cells thereof having the enzyme activity to convert 2, 5 -diketo-D-gluconic acid to 2-keto-L-gulonic acid in a aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resultant mixture.
Claim 7
Dependent Claim
67 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; cultivating the microorganism Brevibacterium nov. sp. ASM-856-4, ATCC 31083, or treated cells thereof having the enzyme activity to convert 2, 5 -diketo-D-gluconic acid to 2-keto-L-gulonic acid in a aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resultant mixture.
Claim 8
Dependent Claim
27 words
A process as claimed in claim 5, wherein the concentration of 2,5-diketo-D-gluconic acid or salt thereof present in said nutrient medium is about 1 to 200 g/l.
Claim 9
Dependent Claim
28 words
A process as claimed in claim 6, wherein said microorganism is cultivated at a temperature of about 20° to 35°C. and at a pH of 4 to 9.
References Cited (Backward Citations)
US2741577A
Cited
US3234105A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-15 | 1979-12-15 | 1980-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-15 | 1983-12-15 | 1984-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-15 | 1987-12-15 | 1988-06-15 | $7700.00 | Window Closed / Lapsed |