US3963573A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing N-acyl-L-methionine
Assignee: Procter and Gamble Co
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Filing: 1975-03-03
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Granted: 1976-06-15
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Expires: 1995-03-03
Abstract
Abstract A process for producing optically pure N-acyl-L-methionine comprising (1) subjecting an N-acyl-D,L-methionine ester to the action of a proteolytic enzyme selected from the group consisting of sulfhydryl proteinases and microbially derived serine proteinases; and (2) separating the resulting N-acyl-L-methionine. The N-acyl group can be chemically removed to provide L-methionine.
Inventors
- 👤 Clyde Eugene Stauffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:15
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
46 words
A process for producing optically pure N-acyl-L-methionine comprising: 1. subjecting a mixture of N-acyl-L-methionine ester and N-acyl-D-methionine ester to the action of a proteolytic enzyme selected from the group consisting of microbially derived serine proteinases; and 2. separating the resulting N-acyl-L-methionine from the unreacted N-acyl-D-methionine ester.
Claim 2
Dependent Claim
46 words
A process for producing optically pure N-acyl-L-methionine comprising: 1. subjecting a mixture of N-acyl-L-methionine ester and N-acyl-D-methionine ester to the action of a proteolytic enzyme selected from the group consisting of microbially derived serine proteinases; and 2. separating the resulting N-acyl-L-methionine from the unreacted N-acyl-D-methionine ester.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the acyl group contains from 1 to 9 carbon atoms.
Claim 4
Dependent Claim
16 words
The process of claim 2 wherein the ester group contains from 1 to 10 carbon atoms.
Claim 5
Dependent Claim
30 words
The process of claim 1 wherein the proteolytic enzyme is a microbially derived serine proteinase which is a member selected from the group consisting of subtilisin Carlsberg and subtilisin BPN.
Claim 6
Dependent Claim
18 words
The process of claim 1 wherein the mixture of N-acyl-L-methionine ester and N-acyl-D-methionine ester comprises N-acetyl-D,L-methionine methyl ester.
Claim 7
Dependent Claim
68 words
A process according to claim 1 for preparing optically pure N-acetyl-L-methionine comprising: 1. subjecting an aqueous solution of a mixture of N-acetyl-D-methionine methyl ester and N-acetyl-L-methionine methyl ester to the action of a proteolytic enzyme which is a member selected from the group consisting of the microbially derived serine proteinases, at a pH in the range of from 5 to 10 ; and 2. separating the resulting N-acetyl-L-methionine.
Claim 8
Dependent Claim
34 words
A process according to claim 6 which is carried out at a pH in the range from about 7 to 8 and at a temperature in the range from about 10°C to about 60°C.
Claim 9
Dependent Claim
24 words
A process according to claim 7 wherein the serine proteinase is a member selected from the group consisting of subtilisin Carlsberg and subtilisin BPN.
Claim 10
Dependent Claim
23 words
A process according to claim 8 wherein the proteinase is subtilisin Carlsberg and the temperature is in the range from 20°C to 40°C.
References Cited (Backward Citations)
US2511867A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-15 | 1979-12-15 | 1980-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-15 | 1983-12-15 | 1984-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-15 | 1987-12-15 | 1988-06-15 | $7700.00 | Window Closed / Lapsed |