US3963572A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fermentative process for the production of L-tryptophan and its derivatives
Assignee: Saab Bofors AB
•
Filing: 1975-02-28
•
Granted: 1976-06-15
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Expires: 1995-02-28
Abstract
Abstract Fermentation process for preparation of L-tryptophan and its derivatives from indole or derivatives thereof wherein the fermentation is carried out in a substrate with methanol as the main source for carbon and is carried out using a certain type of methanol using bacteria.
Inventors
- 👤 Sten Vilhelm Gatenbeck
- 👤 Per Olof Hedman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:28
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/227; C07; C07D; C07D209/00; C07D209/02; C07D209/04; C07D209/10; C07D209/18; C07D209/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/858; Y10S435/874
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
92 words
Method for fermentative preparation of 1-Tryptophan and derivative of the general formula ##SPC1## where R is hydrogen, hydroxyl, alkyl or alkoxy groups from indole or indole derivative of the general formula ##SPC2## where R has the same meaning as above, characterized in that the fermentation is carried out in a substrate consisting essentially of methanol as the main source for carbon and is carried out using methanol using bacteria of a type that assimilates methanol in such a way that the methanol after oxidation reacts with intracellular glycine, whereby serine is formed.
Claim 2
Dependent Claim
92 words
Method for fermentative preparation of 1-Tryptophan and derivative of the general formula ##SPC1## where R is hydrogen, hydroxyl, alkyl or alkoxy groups from indole or indole derivative of the general formula ##SPC2## where R has the same meaning as above, characterized in that the fermentation is carried out in a substrate consisting essentially of methanol as the main source for carbon and is carried out using methanol using bacteria of a type that assimilates methanol in such a way that the methanol after oxidation reacts with intracellular glycine, whereby serine is formed.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the only carbon source for the bacteria consists of methanol.
Claim 4
Dependent Claim
15 words
The method of claim 2 wherein said bacteria belongs to the families Pseudomonas and Methylomonas.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein said bacteria is Pseudomonas AM 1.
Claim 6
Dependent Claim
11 words
The method of claim 2 wherein said bacteria is Methylmonas Methanolica.
Claim 7
Dependent Claim
9 words
The method of claim 1 wherein glycine is added.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein said bacteria belongs to the families Pseudomonas and Methylomonas.
Claim 9
Dependent Claim
12 words
The method of claim 1 wherein said bacteria is Pseudomonas AM 1.
Claim 10
Dependent Claim
11 words
The method of claim 1 wherein said bacteria is Methylomonas Methanolica.
Claim 11
Dependent Claim
14 words
The method of claim 1 wherein said fermentation is carried out under aerobic conditions.
Claim 12
Dependent Claim
17 words
The method of claim 1 wherein said fermentation is carried out at a temperature between 18°-45° C.
Claim 13
Dependent Claim
18 words
The method of claim 1 wherein said fermentation is carried out at a pH of 5.5 - 8.5.
Claim 14
Dependent Claim
18 words
The method of claim 1 wherein said fermentation is carried out at a temperature of 25°- 30° C.
Claim 15
Dependent Claim
18 words
The method of claim 1 wherein said fermentation is carried out at a pH of 6.5 - 7.5.
Claim 16
Dependent Claim
19 words
The method of claim 1 wherein the fermentation is carried out with 0.1 - 1.0% by volume of methanol.
Claim 17
Dependent Claim
18 words
The method of claim 1 wherein the fermentation is carried out with about 0.5% by volume of methanol.
Claim 18
Dependent Claim
12 words
The method of claim 1 wherein said fermentation is a continuous process.
Claim 19
Dependent Claim
19 words
The method of claim 1 wherein the concentration of free indole in the culture medium is below 500 mg/l.
Claim 20
Dependent Claim
19 words
The method of claim 1 wherein the fermentation is a batch process carried out for 30 - 90 hours.
Claim 21
Dependent Claim
9 words
The method of claim 1 wherein 1-Tryptophan is prepared.
Claim 22
Dependent Claim
25 words
The method of claim 1 wherein said derivative of 1-Tryptophan is selected from the group consisting of 5-hydroxy-tryptophan, 5-methoxy tryptophan, 5-methyl tryptophan, and 7-methyl tryptophan.
References Cited (Backward Citations)
US2999051A
Cited
US3808101A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-15 | 1979-12-15 | 1980-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-15 | 1983-12-15 | 1984-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-15 | 1987-12-15 | 1988-06-15 | $7700.00 | Window Closed / Lapsed |