Patent US4900059A successfully refreshed from Google Patents.
US3962058A
Expired / Lapsed
US
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Flat bed isoelectric focusing device
Assignee: Hoffmann La Roche Inc
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Filing: 1975-04-24
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Granted: 1976-06-08
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Expires: 1995-04-24
Abstract
Abstract A flat bed isoelectric focusing device is described. Such device is designed to utilize small volumes, i.e., about 6 ml. and incorporates several critical construction parameters which allow for effecient separations. Thus, the liquid height should be about 2 mm and no more than 4 mm and the length to width ratio must be 15:1 or greater.
Inventors
- 👤 William Donner Denckla
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 21:35
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44795
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
113 words
A flat bed isoelectric focusing device comprising a rectangular separation chamber defined by two side walls, two end walls and a bottom wall wherein the length to width rates of said separation chamber is at least 15:1 and the height of said side and end walls is from 2 to 4 mm; said bottom wall being provided with at least ten symmetrically spaced baffle means, said baffle means extending from one side wall to the other in a plane perpendicular to the longitudinal axis of said separation chamber and having a height of about 1/2 the height of said side and end walls; and electrode means in operative relationship with said separaton chamber.
Claim 2
Dependent Claim
113 words
A flat bed isoelectric focusing device comprising a rectangular separation chamber defined by two side walls, two end walls and a bottom wall wherein the length to width rates of said separation chamber is at least 15:1 and the height of said side and end walls is from 2 to 4 mm; said bottom wall being provided with at least ten symmetrically spaced baffle means, said baffle means extending from one side wall to the other in a plane perpendicular to the longitudinal axis of said separation chamber and having a height of about 1/2 the height of said side and end walls; and electrode means in operative relationship with said separaton chamber.
Claim 3
Dependent Claim
20 words
The device of claim 1 wherein the said length to width ratio of said separation chamber is at least 30:1.
Claim 4
Dependent Claim
18 words
The device of claim 1 wherein the height of said side and end walls is about 2 mm.
Claim 5
Dependent Claim
10 words
The device of claim 1 wherein twenty baffles are provided.
Claim 6
Dependent Claim
24 words
The device of claim 1 wherein the said side and end walls are made of a rigid plastic and the bottom wall is glass.
Claim 7
Dependent Claim
14 words
The device of claim 5 wherein said bottom wall comprises a heat exchange means.
Claim 8
Dependent Claim
18 words
The device of claim 1 wherein the total volume of said separation chamber is less than 10 ml.
Claim 9
Dependent Claim
16 words
The device of claim 1 wherein a cover means is provided during operation to prevent evaporation.
Claim 10
Dependent Claim
53 words
The device of claim 8 wherein said cover means is provided with a number of baffle means having a height of 1/2 the height of said side and end walls, said baffle means being so arranged and constructed as to be in alternating relationship with the said baffle means on said bottom wall.
Claim 11
Dependent Claim
95 words
A method for the isoelectric focusing of a solution of 10 ml. volume or less, said solution containing substances which will migrate under the influence of an applied potential, which method comprises introducing said solution into the separation chamber of a device of claim 1; providing a desired pH gradient across said separation chamber by utilizing carrier ampholytes; providing a potential field across said separation chamber whereby said substances will migrate to their respective isoelectric points and form distinct substance zones; and withdrawing said substances from their respective zones simultaneously and in a segregated manner.
Claim 12
Dependent Claim
17 words
The method of claim 10 wherein said solution comprises one or more proteins and said carrier ampholyte.
Claim 13
Dependent Claim
17 words
The method of claim 11 wherein said protein is present in a concentration of about 1% (w/v).
Claim 14
Dependent Claim
17 words
The method of claim 10 wherein said potential is applied until a constant low conductance is observed.
Claim 15
Dependent Claim
16 words
The method of claim 10 wherein heat exchange is provided during the period of isoelectric focusing.
References Cited (Backward Citations)
US3616456A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-08 | 1979-12-08 | 1980-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-08 | 1983-12-08 | 1984-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-08 | 1987-12-08 | 1988-06-08 | $7700.00 | Window Closed / Lapsed |