US3961894A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test for determination of triiodothyronine
Assignee: Yissum Research Development Co of Hebrew University of Jerusalem
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Filing: 1974-04-22
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Granted: 1976-06-08
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Expires: 1994-04-22
Abstract
Abstract A radioimmunoassay method for the determination of triiodothyronine in human and animal fluids and tissues, particularly in serum. The method involves the separation of triiodothyronine from its binding proteins using a dextran gel column followed by competitive binding between the freed triiodothyronine and radioactive labeled triiodothyronine which remain bound to the gel column for triiodothyronine-specific anti-serum. Bound and free labeled triiodothyronine are separated by washing the column and their ratio is determined based on the amount of radioactivity remaining in the column to that originally added thereto.
Inventors
- 👤 Amirav Gordon
- 👤 Jack Gross
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/81; Y10S436/825
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
142 words
A method for the in vitro determination of triiodothyronine in a biological fluid sample comprising the steps of: a. adding to a column containing a crosslinked dextran gel at a pH of at least about 10.5 or less than about 3 a predetermined quantity of said fluid sample and a quantity of radioactive labeled triiodothyronine, b. washing said column with an aqueous solution having a pH of between about 6 and 9, c. adding to said column a predetermined quantity of antibody to triiodothyronine, d. incubating said column, e. washing said column with an aqueous solution to substantially remove the antibody-bound-triiodothyronine, f. determining the ratio of radioactive labeled triiodothyronine retained in said column after step (e) to that added in step (a), and g. comparing the ratio determined in step (f) to ratios obtained using standard samples containing known amounts of triiodothyronine.
Claim 2
Independent Claim
142 words
A method for the in vitro determination of triiodothyronine in a biological fluid sample comprising the steps of: a. adding to a column containing a crosslinked dextran gel at a pH of at least about 10.5 or less than about 3 a predetermined quantity of said fluid sample and a quantity of radioactive labeled triiodothyronine, b. washing said column with an aqueous solution having a pH of between about 6 and 9, c. adding to said column a predetermined quantity of antibody to triiodothyronine, d. incubating said column, e. washing said column with an aqueous solution to substantially remove the antibody-bound-triiodothyronine, f. determining the ratio of radioactive labeled triiodothyronine retained in said column after step (e) to that added in step (a), and g. comparing the ratio determined in step (f) to ratios obtained using standard samples containing known amounts of triiodothyronine.
Claim 3
Dependent Claim
41 words
A method as in claim 1 wherein said predetermined quantity of said fluid sample and said quantity of radioactive labeled triiodothyronine added to said column in step (a) are first combined and equilibrated before introduction into said column of dextran gel.
Claim 4
Dependent Claim
42 words
A method as in claim 2 wherein said predetermined quantity of antibody of triiodothyronine added to said column in step (c) is in the form of anti-serum prepared in a suitable laboratory animal against a conjugate of triiodothyronine and a suitable carrier.
Claim 5
Dependent Claim
31 words
A method as in claim 2 wherein said dextran gel is cross-linked with epichlorohydrin and has a water regain of from about 1 to 5 grams per gram of dry gel.
Claim 6
Dependent Claim
30 words
A method as in claim 4 wherein the amount of radioactivity contained in said column is measured once after step (c) but before step (e) and again after step (e).
Claim 7
Dependent Claim
20 words
A method as in claim 1 wherein said column in step (a) is at a pH of at least 10.5.
Claim 8
Dependent Claim
36 words
A method as in claim 1 wherein the quantity of radioactive labeled triiodothyronine added to said column in step (a) is predetermined and the amount of radioactivity contained in said column is measured after step (e).
Claim 9
Dependent Claim
20 words
A method as in claim 1 wherein the radioactivity contained in said column is measured before and after step (e).
Claim 10
Dependent Claim
23 words
A method as in claim 1 wherein said aqueous solution used in step (e) has a pH of between about 6 and 9.
Claim 11
Dependent Claim
14 words
A method as in claim 9 wherein said aqueous solution contains a phosphate buffer.
Claim 12
Dependent Claim
13 words
A method as in claim 1 wherein said biological fluid sample is serum.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-08 | 1979-12-08 | 1980-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-08 | 1983-12-08 | 1984-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-08 | 1987-12-08 | 1988-06-08 | $7700.00 | Window Closed / Lapsed |