US3960665A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of proteolytic enzymes
Assignee: Individual
•
Filing: 1973-07-02
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Granted: 1976-06-01
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Expires: 1993-07-02
Abstract
Abstract The invention relates to the production of proteolytic enzymes by aerob submerse fermentation of a Bacillus firmus var. Arosia, and to the use of the enzymes in detergent compositions containing perborates and polyphosphates.
Inventors
- 👤 Karl Johan Stampe Villadsen
- 👤 Karin Pinholt Vestberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 18:21
CPC Classifications
C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
37 words
Process for the production of proteolytic enzymes by aerobic fermentation of a medium containing cabohydrates, comprising using Bacillus firmus var. Arosia, strain NCIB 10557, for the fermentation, and recovering the produced enzymes from the resulting fermentation liquid.
Claim 2
Dependent Claim
37 words
Process for the production of proteolytic enzymes by aerobic fermentation of a medium containing cabohydrates, comprising using Bacillus firmus var. Arosia, strain NCIB 10557, for the fermentation, and recovering the produced enzymes from the resulting fermentation liquid.
Claim 3
Dependent Claim
27 words
Process according to claim 1, in which the fermentation is carried out at a temperature between 28°C and 50°C for a period of 48 to 72 hours.
Claim 4
Dependent Claim
19 words
Process according to claim 1, in which the fermentation liquid is subjected to flocculation before recovering of the enzymes.
Claim 5
Dependent Claim
20 words
Process according to claim 3, in which the flocculation is performed by adding an inorganic salt to the fermented liquid.
Claim 6
Dependent Claim
22 words
Process according to claim 3, in which the flocculated liquid is filtrated, and the enzyme is precipitated by a salting out procedure.
Claim 7
Dependent Claim
26 words
Process according to claim 3, in which the flocculated liquid is filtered, and the enzyme is precipitated by admixing a water-miscible organic solvent to the filtrate.
Claim 8
Dependent Claim
11 words
Proteolytic enzymes as produced by the process according to claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-01 | 1979-12-01 | 1980-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-01 | 1983-12-01 | 1984-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-01 | 1987-12-01 | 1988-06-01 | $7700.00 | Window Closed / Lapsed |