US3953592A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Live influenza virus vaccines and preparation thereof
Assignee: Recherche et Industrie Therapeutiques
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Filing: 1973-09-27
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Granted: 1976-04-27
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Expires: 1993-09-27
Abstract
Abstract Stable H3N2 influenza virus strains completely resistant to serum inhibitors are obtained by passaging at least one time in the allantoic cavity of embryonated eggs in the presence of serum a recombinant strain previously obtained from an H3N2 influenza strain and the A/PR8 influenza virus strain and isolating the so-obtained serum inhibitors resistant strains. The obtained strains are useful for vaccine production.
Inventors
- 👤 Julien Peetermans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:30
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; A61K2039/51; A61K2039/525; A61K2039/5254; A61K2039/54; A61K2039/541; A61K2039/543; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
74 words
An attenuated influenza virus vaccine effective on intranasal administration comprising an effective amount of a serum inhibitor-resistant strain of influenza A virus obtained by passaging in the allantoic cavity of embryonated eggs a recombinant strain obtained from H 3 N 2 influenza and A/PR 8 influenza, which strain is sensitive to serum inhibitors, said passaging being made in the presence of serum until a serum inhibitor resistant strain is obtained, and a carrier therefor.
Claim 2
Dependent Claim
74 words
An attenuated influenza virus vaccine effective on intranasal administration comprising an effective amount of a serum inhibitor-resistant strain of influenza A virus obtained by passaging in the allantoic cavity of embryonated eggs a recombinant strain obtained from H 3 N 2 influenza and A/PR 8 influenza, which strain is sensitive to serum inhibitors, said passaging being made in the presence of serum until a serum inhibitor resistant strain is obtained, and a carrier therefor.
Claim 3
Dependent Claim
16 words
A vaccine according to claim 1, where the H 3 N 2 influenza strain is A/England/42/72.
Claim 4
Dependent Claim
20 words
A vaccine according to claim 2, where the A/PR 8 influenza strain is the Mount Sinai A/PR 8 /34 strain.
Claim 5
Dependent Claim
13 words
A vaccine according to claim 3, where the recombinant is the MRC-2 strain.
Claim 6
Dependent Claim
14 words
A vaccine according to claim 4, wherein the embryonated eggs are embryonated chicken eggs.
Claim 7
Dependent Claim
13 words
A vaccine according to claim 5, where the serum is guinea pig serum.
Claim 8
Dependent Claim
39 words
A vaccine according to claim 6, where the passages are carried out in normal saline and by varying the serum and virus dilutions and isolating the virus from the culture having the highest serum concentration and highest virus dilution.
Claim 9
Dependent Claim
56 words
A method of preparing an attenuated influenza virus vaccine comprising incubating in the allantoic cavity of embryonated eggs a serum-inhibitor-resistant strain of influenza A Virus obtained according to claim 1, said incubation being for a period of time sufficient to permit growth of a larger amount of said virus strain and harvesting the resulting virus material.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-27 | 1979-10-27 | 1980-04-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-27 | 1983-10-27 | 1984-04-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-27 | 1987-10-27 | 1988-04-27 | $7700.00 | Window Closed / Lapsed |