US3949073A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution
Assignee: Leland Stanford Junior University
•
Filing: 1974-11-18
•
Granted: 1976-04-06
•
Expires: 1994-11-18
Abstract
Abstract A method for augmenting hard or soft connective tissue, such as skin, tendon, cartilage, bone or interstitium, in a living mammal comprising implanting a proteolytic enzyme-solubilized, purified, native, in situ polymerizable collagen solution into the mammal at the augmentation site. The solution polymerizes at the site into a stable, non-reactive fibrous mass of tissue which is rapidly colonized by host cells and vascularized.
Inventors
- 👤 John R. Daniels
- 👤 Terry R. Knapp
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:44
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K38/00; Y; Y10; Y10S; Y10S128/00; Y10S128/08; Y10S623/00; Y10S623/92
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
39 words
Method of augmenting connective tissue in a living mammal comprising administering a solution of solubilized, purified, native, in situ polymerizable collagen to the mammal at the augmentation site which polymerizes at said site into a fibrous mass of tissue.
Claim 2
Dependent Claim
39 words
Method of augmenting connective tissue in a living mammal comprising administering a solution of solubilized, purified, native, in situ polymerizable collagen to the mammal at the augmentation site which polymerizes at said site into a fibrous mass of tissue.
Claim 3
Dependent Claim
16 words
The method of claim 1 in which the solution is implanted into the mammal by injection.
Claim 4
Dependent Claim
16 words
The method of claim 1 in which the solution is administered by coating the augmentation site.
Claim 5
Dependent Claim
15 words
The method of claim 1 in which the collagen source is heterologous skin or tendon.
Claim 6
Dependent Claim
12 words
The method of claim 1 in which the mammal is a human.
Claim 7
Dependent Claim
19 words
The method of claim 1 in which the concentration of collagen in the solution is up to 20 mg/ml.
Claim 8
Dependent Claim
21 words
The method of claim 1 in which the concentration of collagen in the solution is about 12 to about 15 mg/ml.
Claim 9
Dependent Claim
18 words
The method of claim 1 in which the pH of the solution is about 6 to about 8.
Claim 10
Dependent Claim
20 words
The method of claim 1 in which the solution contains a polymerization promoter which causes the solution to be isotonic.
Claim 11
Dependent Claim
15 words
The method of claim 9 in which the promoter is a buffered, neutral salt solution.
Claim 12
Dependent Claim
23 words
The method of claim 10 in which the buffered, neutral salt solution is a solution of NaCl and NaHPO 4 , pH 7.4.
Claim 13
Dependent Claim
18 words
The method of claim 1 in which the collagen has been solubilized by treatment with a proteolytic enzyme.
Claim 14
Dependent Claim
34 words
The method of claim 12 in which the treatment with a proteolytic enzyme is made in an acid medium, pH of 1 to 4.5 at a temperature in the range of 0°C to 15°C.
Claim 15
Dependent Claim
11 words
The method of claim 13 in which the temperature is 11°C.
Claim 16
Dependent Claim
12 words
The method of claim 14 in which the proteolytic enzyme is pepsin.
Claim 17
Dependent Claim
16 words
The method of claim 1 in which the solubilized collagen has been purified by salt precipitation.
Claim 18
Dependent Claim
21 words
The method of claim 9 in which the temperature of the solution is kept below about 5°C until it is administered.
Claim 19
Dependent Claim
21 words
The method of claim 1 in which particles of insoluble collagen microfibrils are added to the solution before it is administered.
Claim 20
Dependent Claim
24 words
The method of claim 1 in which the solution is administered to the skin as a sealer to cover a burn or an abrasion.
Claim 21
Dependent Claim
16 words
The method of claim 19 in which the solution contains a drug to prevent secondary infection.
Claim 22
Dependent Claim
55 words
The method of claim 1 in which the collagen has been solubilized by treatment with a proteolytic enzyme, the concentration of collagen in the solution is up to 20 mg/ml, the pH of the solution is about 6 to about 8 and the solution contains a polymerization promoter which causes the solution to be isotonic.
References Cited (Backward Citations)
US3469003A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-06 | 1979-10-06 | 1980-04-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-06 | 1983-10-06 | 1984-04-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-06 | 1987-10-06 | 1988-04-06 | $7700.00 | Window Closed / Lapsed |