US3948730A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Catalasimeter
Assignee: University of Quebec in Montreal
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Filing: 1974-07-11
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Granted: 1976-04-06
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Expires: 1994-07-11
Abstract
Abstract An apparatus and a method for determining the concentration of a first reactant in a medium. A disc is saturated with a solution containing the first reactant and immersed into a second reactant fluid. A chemical reaction occurs between the first and second reactants to produce a gas which is trapped by the disc. The disc is buoyed to the surface of the second reactant fluid by the gas produced. The process is electronically timed using photocells to start and stop an electronic clock and a digital display displays the time elapsed in tenths of seconds.
Inventors
- 👤 Marcel Gagnon
- 👤 Michel Baril
- 👤 Francois-Gros D'Aillon
- 👤 Claude Savoie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:47
CPC Classifications
G; G01; G01N; G01N31/00; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
257 words
A method of measuring the concentration of a catalase enzyme in a medium by reacting it with H 2 O 2 of a predetermined concentration to produce a gas, said method comprising the steps of: a. saturating a gas-trapping object of known weight with said medium containing said catalase enzyme; l b. placing said gas-trapping object into an open container which contains, to a predetermined depth, a quantity of said H 2 O 2 ; and c. measuring the time taken for said gas-trapping object to sink into said H 2 O.sub. 2, and the time taken to produce a quantity of gas sufficient to buoy said gas-trapping object to the surface of said H 2 O 2 , wherein the time taken for said gas-trapping object to sink into said H 2 O 2 and to be buoyed to the surface by said gas is proportional to the concentration of said catalase enzyme in said medium; said time measurement being effected by: i. breaking at least one light beam with said gas-trapping object upon its entry into said fluid to thereby start a counter; and ii. breaking said at least one light beam with said gas-trapping object when said gas-trapping object is buoyed to said surface, to thereby stop said counter, thereby determining the elapsed time between successive breakings of said at least one light beam; and d. said time measurement being delayed for a predetermined time after said gas-trapping object has broken said beam upon entry into said H 2 O 2 in said container.
Claim 2
Independent Claim
257 words
A method of measuring the concentration of a catalase enzyme in a medium by reacting it with H 2 O 2 of a predetermined concentration to produce a gas, said method comprising the steps of: a. saturating a gas-trapping object of known weight with said medium containing said catalase enzyme; l b. placing said gas-trapping object into an open container which contains, to a predetermined depth, a quantity of said H 2 O 2 ; and c. measuring the time taken for said gas-trapping object to sink into said H 2 O.sub. 2, and the time taken to produce a quantity of gas sufficient to buoy said gas-trapping object to the surface of said H 2 O 2 , wherein the time taken for said gas-trapping object to sink into said H 2 O 2 and to be buoyed to the surface by said gas is proportional to the concentration of said catalase enzyme in said medium; said time measurement being effected by: i. breaking at least one light beam with said gas-trapping object upon its entry into said fluid to thereby start a counter; and ii. breaking said at least one light beam with said gas-trapping object when said gas-trapping object is buoyed to said surface, to thereby stop said counter, thereby determining the elapsed time between successive breakings of said at least one light beam; and d. said time measurement being delayed for a predetermined time after said gas-trapping object has broken said beam upon entry into said H 2 O 2 in said container.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein said predetermined concentration of H 2 O 2 is 3% by volume.
References Cited (Backward Citations)
US3764479A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-06 | 1979-10-06 | 1980-04-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-06 | 1983-10-06 | 1984-04-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-06 | 1987-10-06 | 1988-04-06 | $7700.00 | Window Closed / Lapsed |