US3940317A
Expired / Lapsed
US
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Method of isolation of lysozyme
Assignee: Individual
•
Filing: 1973-04-06
•
Granted: 1976-02-24
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Expires: 1993-04-06
Abstract
Abstract Lysozyme from human, avian, non-human mammalian, and vegatable sources is isolated from said sources and provided in a high degree of purity by adsorbing the lysozyme upon chitin obtained from Loligo vulgaris by contacting said chitin with an acidic aqueous suspension of the crude lysozyme containing material, and eluting the pure lysozyme from said chitin with aqueous acid.
Inventors
- 👤 Friedrich D. Katz
- 👤 Louis Fishman
- 👤 Milton Levy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:12
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2462; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
46 words
A method of isolating lysozyme from a source thereof comprising: a. treating an aqueous suspension of said lysozyme containing source material with deaminated squid chitin, b. separating the chitin from said suspension, washing said chitin with aqueous acid and separating said aqueous acid from said chitin.
Claim 2
Independent Claim
46 words
A method of isolating lysozyme from a source thereof comprising: a. treating an aqueous suspension of said lysozyme containing source material with deaminated squid chitin, b. separating the chitin from said suspension, washing said chitin with aqueous acid and separating said aqueous acid from said chitin.
Claim 3
Dependent Claim
15 words
A process according to claim 1 additionally comprising removing the lysozyme from said acid wash.
Claim 4
Dependent Claim
80 words
A method according to claim 1 wherein the chitin is obtained by the successive steps of: a. homogenizing dried, flesh-free pens of the squid, Loligo vulgaris in aqueous saline, b. discarding the aqueous supernate, c. resuspending the residual material in dilute aqueous organic acid and rehomogenizing, d. discarding the aqueous supernate, e. drying the residue designated as chitin, f. powdering the chitin to pass through a mesh of 100 to 400 mesh size, g. reacting the chitin with nitrous acid.
Claim 5
Dependent Claim
21 words
A process according to claim 3 wherein the source of nitrous acid is sodium nitrite in the presence of an acid.
Claim 6
Dependent Claim
13 words
A process according to claim 4 wherein the acid is glacial acetic acid.
Claim 7
Dependent Claim
37 words
A process according to claim 1 which comprises additionally suspending lysozyme containing material in an aqueous medium of pH between 3.8 and 4.6 and separating therefrom the water insoluble material, before treating said lysozyme suspension with chitin.
Claim 8
Dependent Claim
33 words
A process according to claim 6 wherein the suspending step comprises homogenizing the lysozyme containing material in dilute aqueous acid, separating the aqueous solution from other fractions present and preserving said aqueous fractions.
Claim 9
Dependent Claim
17 words
A process according to claim 6 wherein the separation step comprises centrifuging and preserving the aqueous portion.
Claim 10
Dependent Claim
34 words
A process according to claim 6 which comprises: a. charging the deaminated chitin to a chromatograhic column, b. charging the aqueous solution containing lysozyme to said column, c. eluting said column with aqeuous acid.
Claim 11
Dependent Claim
22 words
A process according to claim 9 additionally comprising the step of washing said column with water prior to elution with aqueous acid.
Claim 12
Dependent Claim
15 words
A process according to claim 9 additionally comprising separating the lysozyme from the acidic eluate.
Claim 13
Dependent Claim
17 words
A process according to claim 11 wherein the acid is a substantially volatile water soluble organic acid.
Claim 14
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is human tissues.
Claim 15
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is human placenta.
Claim 16
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is human plasma.
Claim 17
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is avian egg-white.
Claim 18
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is mammalian milk.
Claim 19
Dependent Claim
14 words
A process according to claim 9 wherein the lysozyme source material is mammalian blood.
Claim 20
Dependent Claim
13 words
A process according to claim 9 wherein the lysozyme source material is vegetables.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-02-23 | 1979-08-25 | 1980-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-02-23 | 1983-08-25 | 1984-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-02-23 | 1987-08-25 | 1988-02-24 | $7700.00 | Window Closed / Lapsed |