Vitro net bioxynthesis of chlorophyll and grana

Claim 2

A process for in vitro net biosynthesis of chlorophyll and grana, said process comprising the following steps in relative sequence: a. irradiating etiolated plant tissue with visible light to precipitate formation of developing chloroplasts in said etiolated tissue; b. homogenizing said tissue in an environment having available oxygen and in a buffer comprising biosynthetic cofactors including, coenzyme A, glutathione, potassium, inoganic phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.9 to 8.2, as measured at ambient temperature; c. isolating developing chloroplasts from the homogenate of step (b); and d. incubating, while irradiating with visible light, said developing chloroplasts in an environment having available oxygen and in a buffer comprising biosynthetic cofactors including, δ-aminolevulinic acid, coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium nicotinamide adenine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.6 to 7.8, as measured at ambient

Claim 3

The process of claim 1 further including the step of isolating

Claim 4

The process according to claim 1 wherein step (a) includes irradiating said tissue with light of an intensity of from about 100 to about 300 foot-candles, for duration of from about 2 to about 5 hours, and at a

Claim 5

The process according to claim 3 wherein the intensity of light is in

Claim 6

The process according to claim 3 wherein the duration is about 4 hours.

Claim 7

The process according to claim 3 wherein the temperature is in the

Claim 8

The process according to claim 1 wherein step (b) includes homogenizing

Claim 9

The process according to claim 1 wherein step (d) includes irradiating with visible light of from about 1 to about 50 foot-candles and incubating

Claim 10

The process according to claim 8 wherein the intensity of light is in

Claim 11

The process according to claim 8 wherein the temperature is in the

Claim 12

The process according to claim 1 wherein step (b) includes homogenizing

Claim 13

The process according to claim 1 wherein step (d) includes incubating

Claim 14

The process according to claim 1 wherein said etiolated plant tissue is

Claim 15

The process according to claim 13 wherein said higher plant tissue is

Claim 16

The process according to claim 14 wherein said cotyledon tissue is

Claim 17

The process for in vitro net biosynthesis of chlorophyll a, chlorophyll b and grana having chlorophyll a and chlorophyll b associated therewith, said process comprising the following steps in relative sequence: a. irradiating etiolated plant tissue with visible light of an intensity of from about 100 to about 300 foot-candles, for a duration of from about 2 to about 5 hours, at a temperature of from about 20° to about 35°C., to precipitate formation of developing chloroplasts in said etiolated tissue; b. homogenizing said tissue in an environment having available oxygen and in a buffer comprising biosynthetic cofactors including, coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.9 to 8.2, as measured at ambient temperature; c. isolating developing chloroplasts from the homogenate of step (b); and d. incubating, while irradiating with visible light of an intensity of from about 1 to about 50 foot-candles, said developing chloroplasts at a temperature of from about 15° to about 35°C., in an environment having available oxygen, and in a buffer comprising biosynthetic cofactors including, δ-aminolevulinic acid, coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenosine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.6 to 7.8, as measured at ambient temperature, to produce chlorophyll a, chlorophyll b and grana having chlorophyll a and

Claim 18

The process for in vitro net biosynthesis of chlorophyll a, chlorophyll b and grana having chlorophyll a and chlorophyll b associated therewith, said process comprising the following steps in relative sequence: a. irradiating etiolated plant tissue with visible light of an intensity of from about 100 to about 300 foot-candles, for a duration of from about 2 to about 5 hours, at a temperature of from about 20° to about 35°C., to precipitate formation of developing chloroplasts in said etiolated tissue; b. homogenizing said tissue in an environment having available oxygen and in a buffer comprising biosynthetic cofactors including, coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.9 to 8.2, as measured at ambient temperature; c. isolating developing chloroplasts from the homogenate of step (b); and d. incubating, while irradiating with visible light of an intensity of from about 1 to about 50 foot-candles, said developing chloroplasts at a temperature of from about 15° to about 35°C., in an environment having available oxygen, and in a buffer comprising biosynthetic cofactors including, δ-aminolevulinic acid, coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenosine dinucleotide and adenosine triphosphate, which buffer has a pH of from 7.6 to 7.8, as measured at ambient temperature, to produce chlorophyll a, chlorophyll b and grana having chlorophyll a and

Claim 19

The process according to claim 16 further including the step of isolating chlorophyll a, chlorophyll b and grana having chlorophyll a and chlorophyll b associated therewith from the buffer subsequent to step (d).