US3932671A
Expired / Lapsed
US
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Process for producing protein hydrolyzate
Assignee: Kikkoman Corp
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Filing: 1973-03-20
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Granted: 1976-01-13
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Expires: 1993-03-20
Abstract
Abstract A protein hydrolyzate having a high free amino acid content and an excellent flavor can be obtained when salt-resistant lactic acid bacteria are additionally used in the absence or presence of at most 12 percent by weight of sodium chloride based on a volume of an aqueous medium in obtaining the protein hydrolyzate from proteinaceous materials by hydrolysis with proteolytic enzymes.
Inventors
- 👤 Tamotsu Yokotsuka
- 👤 Takashi Iwaasa
- 👤 Tadao Okami
- 👤 Minoru Noda
- 👤 Mitsuharu Fujii
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:23
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/06; A; A23; A23J; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23J3/341; A23J3/343; A23J3/344
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
92 words
In a process for producing a protein hydrolyzate from proteinaceous materials by hydrolysis with proteolytic enzymes in an aqueous medium to form a protein partial hydrolyzate containing polypeptides the improvement comprising inoculating and culturing salt-resistant lactic acid bacteria selected from the group consisting of Pediococcus halophilus, Pediococcus soyae, Tetracoccus soyae and Streptococcus thermophilus in the aqueous medium containing said protein partial hydrolyzate and up to 12 percent by weight of sodium chloride based on the volume of the aqueous medium until the free amino acid content in the aqueous medium is increased.
Claim 2
Dependent Claim
92 words
In a process for producing a protein hydrolyzate from proteinaceous materials by hydrolysis with proteolytic enzymes in an aqueous medium to form a protein partial hydrolyzate containing polypeptides the improvement comprising inoculating and culturing salt-resistant lactic acid bacteria selected from the group consisting of Pediococcus halophilus, Pediococcus soyae, Tetracoccus soyae and Streptococcus thermophilus in the aqueous medium containing said protein partial hydrolyzate and up to 12 percent by weight of sodium chloride based on the volume of the aqueous medium until the free amino acid content in the aqueous medium is increased.
Claim 3
Dependent Claim
99 words
In a process for producing a protein hydrolyzate from proteinaceous material by hydrolysis with proteolytic enzymes in an aqueous medium to form a protein partial hydrolyzate containing polypeptides the improvements comprising adding cells of salt-resistant lactic acid bacteria selected from the group consisting of Pediococcus halophilus, Pediococcus soyae, Tetracoccus soyae and Streptococcus thermophilius to the aqueous medium containing said protein partial hydrolyzate and 0-12 percent by weight of sodium chloride based on the volume of the aqueous medium and allowing said bacteria to contact and break down the polypeptides to increase the free amino acid content of the hydrolyzate.
Claim 4
Dependent Claim
99 words
In a process for producing a protein hydrolyzate from proteinaceous material by hydrolysis with proteolytic enzymes in an aqueous medium to form a protein partial hydrolyzate containing polypeptides the improvements comprising adding cells of salt-resistant lactic acid bacteria selected from the group consisting of Pediococcus halophilus, Pediococcus soyae, Tetracoccus soyae and Streptococcus thermophilius to the aqueous medium containing said protein partial hydrolyzate and 0-12 percent by weight of sodium chloride based on the volume of the aqueous medium and allowing said bacteria to contact and break down the polypeptides to increase the free amino acid content of the hydrolyzate.
Claim 5
Dependent Claim
26 words
A process according to claim 1, wherein the inoculation and culture of the salt-resistant bacteria are conducted after substantial termination of the hydrolysis of proteinaceous materials.
Claim 6
Dependent Claim
38 words
A process according to claim 1, wherein the salt-resistant lactic acid bacteria are those which are resistant to sodium chloride in a concentration of 10 percent by weight based on a volume of an aqueous medium or higher.
Claim 7
Dependent Claim
51 words
A process according to claim 1, wherein the salt-resistant lactic acid bacteria are those selected from the group consisting of Pediococcus halophilus ATCC 21786, Pediococcus soyae, ATCC 13621, Pediococcus soyae ATCC 13622, Pediococcus soyae, ATCC 13623, Tetracoccus soyae ATCC 21787, Streptococcus thermophilus IAM 1047, Streptococcus thermophilus and Streptococcus thermophilus ATCC 19282.
Claim 8
Dependent Claim
34 words
A process according to claim 1, wherein the culture of salt-resistant bacteria is conducted at a pH of 5 to 8 and at a temperature of 15° to 60°C. for 1 to 15 days.
Claim 9
Dependent Claim
27 words
A process according to claim 1, wherein the proteolytic enzyme is one member selected from the group consisting of enzymes produced by aspergilli, streptomyces, pepsin and papain.
Claim 10
Dependent Claim
30 words
A process according to claim 1, wherein the proteinaceous material is one member selected from the group consisting of soybean, wheat gluten, milk casein, fish meal, cattle meat and yeast.
Claim 11
Dependent Claim
37 words
A process according to claim 2, wherein the adding of the cells of salt-resistant bacteria is conducted at a pH of 5 to 8 and at a temperature of 15° to 60°C. for 10 to 72 hours.
Claim 12
Dependent Claim
26 words
A process according to claim 2, wherein the addition of the cells of said bacteria is conducted after substantial termination of the hydrolysis of proteinaceous materials.
Claim 13
Dependent Claim
38 words
A process according to claim 2, wherein the salt-resistant lactic acid bacteria are those which are resistant to sodium chloride in a concentration of 10 percent by weight based on a volume of an aqueous medium or higher.
Claim 14
Dependent Claim
49 words
A process according to claim 2, wherein the salt-resistant lactic acid bacteria are those selected from the group consisting of Pediococcus halophilus ATCC 21786, Pediococcus soyae ATCC 13621, Pediococcus soyae ATCC 13622, Pediococcus soyae ATCC 13623, Tetracoccus soyae ATCC 21787, Streptococcus thermophilus IAM 1047 and Streptococcus thermophilus ATCC 19282.
Claim 15
Dependent Claim
33 words
A process according to claim 2, wherein amount of the cells of salt-resistant lactic acid bacteria to be added is 10 8 to 10 10 in terms of number of cells per milliliter.
Claim 16
Dependent Claim
27 words
A process according to claim 2, wherein the proteolytic enzyme is a member selected from the group consisting of enzymes produced by aspergilli, streptomyces, pepsin and papain.
Claim 17
Dependent Claim
30 words
A process according to claim 2, wherein the proteinaceous material is one member selected from the group consisting of soybean, wheat gluten, milk casein, fish meal, cattle meat and yeast.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-01-12 | 1979-07-14 | 1980-01-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-01-12 | 1983-07-14 | 1984-01-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-01-12 | 1987-07-14 | 1988-01-13 | $7700.00 | Window Closed / Lapsed |