US3932229A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of sample application to gel electrophoresis media
Assignee: EMD Millipore Corp
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Filing: 1974-11-15
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Granted: 1976-01-13
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Expires: 1994-11-15
Abstract
Abstract The invention concerns a method for the application of sample liquids to a gel type electrophoretic medium which leaves substantially no application artifact in the final electrophoretic pattern. The method consists of placing a cover sheet, having slits formed therein at the desired sample application locations, on the upper surface of the gel. An excess of sample is placed over each slit and allowed to remain in place for a fixed time period. It is then blotted to remove excess sample material and the cover is removed. Sample materials imbibed into the gel will then be appropriately located and in predetermined amounts depending on the gel porosity and time that the sample is in contact with the gel.
Inventors
- 👤 Joseph D. Grandine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:24
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44743
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
130 words
The method of applying a sample liquid to a gel electrophoresis medium which comprises in combination the steps of: a. placing a cover sheet in contact with the surface of such gel medium, said sheet having a slit formed therein at the desired sample location; b. placing an amount of sample in excess of that to be absorbed by said medium over said slit, c. beginning measurement of a predetermined time when said excess sample is in place over said slit; d. blotting the unabsorbed sample from said slit at the end of the measurement of said predetermined time, whereby said excess sample is in place over said slit for a fixed and determined period; and e. removing said cover sheet from contact with the surface of said gel medium
Claim 2
Independent Claim
130 words
The method of applying a sample liquid to a gel electrophoresis medium which comprises in combination the steps of: a. placing a cover sheet in contact with the surface of such gel medium, said sheet having a slit formed therein at the desired sample location; b. placing an amount of sample in excess of that to be absorbed by said medium over said slit, c. beginning measurement of a predetermined time when said excess sample is in place over said slit; d. blotting the unabsorbed sample from said slit at the end of the measurement of said predetermined time, whereby said excess sample is in place over said slit for a fixed and determined period; and e. removing said cover sheet from contact with the surface of said gel medium
Claim 3
Dependent Claim
23 words
The method defined in claim 1 in which said cover sheet is made of a material which is not wetted by said sample.
Claim 4
Dependent Claim
32 words
The method defined in claim 1 in which said cover sheet has a plurality of slits formed therein, whereby a plurality of samples may be applied to the surface of said gel.
Claim 5
Dependent Claim
25 words
The method defined in claim 3 which includes the steps of applying all of said samples simultaneously to said slits and simultaneously blotting said samples.
Claim 6
Independent Claim
140 words
The method of applying sample liquids to a gel electrophoresis medium which comprises, in combination the steps of: a. placing a cover sheet in contact with the surface of said gel medium, said sheet having a plurality of slits formed therein at the desired sample application locations; b. sequentially placing an amount of sample in excess of that to be absorbed by said medium over each slit; c. beginning measurement of a predetermined time period for each slit when said excess sample is in place over said slit; d. blotting the unabsorbed sample from each slit at the end of the measurement of the predetermined time for said slit, whereby said excess sample is in place over said slit for a fixed and predetermined period; and e. removing the cover sheet from contact with the surface of said gel medium.
Claim 7
Independent Claim
140 words
The method of applying sample liquids to a gel electrophoresis medium which comprises, in combination the steps of: a. placing a cover sheet in contact with the surface of said gel medium, said sheet having a plurality of slits formed therein at the desired sample application locations; b. sequentially placing an amount of sample in excess of that to be absorbed by said medium over each slit; c. beginning measurement of a predetermined time period for each slit when said excess sample is in place over said slit; d. blotting the unabsorbed sample from each slit at the end of the measurement of the predetermined time for said slit, whereby said excess sample is in place over said slit for a fixed and predetermined period; and e. removing the cover sheet from contact with the surface of said gel medium.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-01-12 | 1979-07-14 | 1980-01-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-01-12 | 1983-07-14 | 1984-01-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-01-12 | 1987-07-14 | 1988-01-13 | $7700.00 | Window Closed / Lapsed |