US3929579A
Expired / Lapsed
US
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Dental caries-inducing microorganism cells lytic enzyme
Assignee: Sumitomo Pharma Co Ltd
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Filing: 1972-07-11
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Granted: 1975-12-30
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Expires: 1992-07-11
Abstract
Abstract An enzyme being capable of lysing cells of dental cariesinducing microorganisms and a method for producing the same by cultivating a microorganism belonging to the genus Streptomyces, especially wild type strains of Streptomyces diastatochromogenes, Streptomyces farinosus, Streptomyces globisporus and Streptomyces griseus var. H-402, compositions for preventing and treating dental caries containing the said enzyme and methods for preventing and treating dental caries.
Inventors
- 👤 Yoshio Yoshimura
- 👤 Kanae Yokogawa
- 👤 Shigeo Kawata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:29
CPC Classifications
A; A61; A61Q; A61Q11/00; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/66; C; C12; C12N; C12N9/00; C12N9/14; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/894; Y10S435/897
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
34 words
AN ENZYME CAPABLE OF LYSING CELLS OF CARIOGENIC STREPTOCOCCI PRODUCED BY CULTIVATION OF A STREPTOMYCES MICROORGANIISM SELECTED FROM THE GROUP CONSISTING OF STREPTOMYCES IDASTATOCHROMOGENES (ATCC 21487), STREPTOMYCES FARINOSUS (ATCC 21482), STREPTOMYCES GLOBISPORUS (ATCC 21553)
Claim 2
Dependent Claim
34 words
AN ENZYME CAPABLE OF LYSING CELLS OF CARIOGENIC STREPTOCOCCI PRODUCED BY CULTIVATION OF A STREPTOMYCES MICROORGANIISM SELECTED FROM THE GROUP CONSISTING OF STREPTOMYCES IDASTATOCHROMOGENES (ATCC 21487), STREPTOMYCES FARINOSUS (ATCC 21482), STREPTOMYCES GLOBISPORUS (ATCC 21553)
Claim 3
Dependent Claim
15 words
The enzyme according to claim 1, wherein said microorganism is Streptomyces diastatochromogenes or mutant thereof.
Claim 4
Dependent Claim
15 words
The enzyme according to claim 1, wherein said microorganism is Streptomyces farinosus or mutant thereof.
Claim 5
Dependent Claim
15 words
The enzyme according tO claim 1, wherein said microorganism is Streptomyces globisporus or mutant thereof.
Claim 6
Dependent Claim
17 words
The enzyme according to claim 1, wherein said microorganism is Streptomyces griseus var. H-402 or mutant thereof.
Claim 7
Independent Claim
57 words
A method of producing an enzyme capable of lysing cells of dental caries-inducing microorganisms which comprises cultivating a Streptomyces microorganism selected from the group consisting of Streptomyces diastatochromogenes (ATCC 21481), Streptomyces farinosus (ATCC 21482), Streptomyces globisporus (ATCC 21553) and Streptomyces griseus Var. H-402 (ATCC 21483) and mutants thereof; and recovering the produced enzyme from the culture broth.
Claim 8
Independent Claim
57 words
A method of producing an enzyme capable of lysing cells of dental caries-inducing microorganisms which comprises cultivating a Streptomyces microorganism selected from the group consisting of Streptomyces diastatochromogenes (ATCC 21481), Streptomyces farinosus (ATCC 21482), Streptomyces globisporus (ATCC 21553) and Streptomyces griseus Var. H-402 (ATCC 21483) and mutants thereof; and recovering the produced enzyme from the culture broth.
Claim 9
Dependent Claim
15 words
The method according to claim 6, wherein said microorganism is Streptomyces diastatochromogenes or mutant thereof.
Claim 10
Dependent Claim
15 words
The method according to claim 6, wherein said microorganism is Streptomyces farinosus or mutant thereof.
Claim 11
Dependent Claim
15 words
The method according to claim 6, wherein said microorganism is Streptomyces globisporus or mutant thereof.
Claim 12
Dependent Claim
17 words
The method according to claim 6, wherein said microorganism is Streptomyces griseus var. H-402 or mutant thereof.
Claim 13
Dependent Claim
23 words
The method according to claim 6, wherein said cultivation is carried out at 20* to 40*C. for several hours to several ten days.
Claim 14
Dependent Claim
39 words
A method for stabilizing an enzyme in accordance with claim 1 which comprises adding at least one of stabilizer selected from the group consisting of sorbitol, sucrose, mannose, sorbose, proline, soluble starch to the enzyme or the enzyme-containing composition.
Claim 15
Dependent Claim
32 words
A stable enzyme solution or powder comprising an enzyme in accordance with claim 1 and at least one of stabilizer selected from the group consisting of sorbitol, sucrose, mannose, sorbose, and proline.
Claim 16
Dependent Claim
18 words
A stable enzyme powder comprising an enzyme in accordance with claim 1 and soluble starch as a stabilizer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |