US3928141A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for nitroblue tetrazolium (NBT) tests for chronic granulomatous disease and related syndromes
Assignee: ATLAS LABORATORIES Ltd C/O
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Filing: 1975-03-06
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Granted: 1975-12-23
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Expires: 1995-03-06
Abstract
Abstract The classic nitroblue tetrazolium (NBT) test utilizes the sensitivity of the NBT dye to the Leukocyte specific Oxidase activity and is used to evaluate the polymorphonuclear leukocyte activity in granulomatous disease and related syndromes. PMN leukocytes, monocytes and eosinophilis from patients with CGD fail to reduce NBT dye (yellow) to blue formazan (deep blue), due to faulty oxidation of the nucleotides by their oxidases. The NBT as a hydrogen acceptor does not accept hydrogen directly from a substrate, but through an oxidase. Improved results are obtained in a greater number of related diseases by the use of a mixture of a reagent such as NBT mixed in distilled water to which dried human plasma is added. This is an unstimulated solution to which a blood sample is added and the reactions studied. The addition of a selected Lipopolysaccharide provides a stimulated solution used in the diagnosis of various diseases.
Inventors
- 👤 Zenon Sosnowski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 06:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849; Y10S435/88; Y10S435/881; Y10S435/882
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
78 words
A METHOD FOR CONDUCTING NITROBLUE TETRAZOLIUM TESTS CONSISTING OF THE STEPS OF MIXING A QUANTITY OF NITROBLUE TETRAZOLIUM DYE WITH DISTILLED WATER, THEN ADDING A QUANTITY OF DRIED HUMAN PLASMA AND THEN ADDING A QUANTITY OF HEPARINZED BLOOD TO BE TESTED, LETTING THE RESULTS OF THE MIXTURE INCUBATE AT A TEMPERATURE SUFFICIENT TO PERMIT INCUBATION, PREPARING A SMEAR FROM THE MIXTURE, STRAINING THE SMEAR IN A CONVENTIONAL MANNER AND THEN COUNTING THE P.M.N.-LEUKOCYTES HAVING A DARK BLUE DEPOSIT.
Claim 2
Independent Claim
78 words
A METHOD FOR CONDUCTING NITROBLUE TETRAZOLIUM TESTS CONSISTING OF THE STEPS OF MIXING A QUANTITY OF NITROBLUE TETRAZOLIUM DYE WITH DISTILLED WATER, THEN ADDING A QUANTITY OF DRIED HUMAN PLASMA AND THEN ADDING A QUANTITY OF HEPARINZED BLOOD TO BE TESTED, LETTING THE RESULTS OF THE MIXTURE INCUBATE AT A TEMPERATURE SUFFICIENT TO PERMIT INCUBATION, PREPARING A SMEAR FROM THE MIXTURE, STRAINING THE SMEAR IN A CONVENTIONAL MANNER AND THEN COUNTING THE P.M.N.-LEUKOCYTES HAVING A DARK BLUE DEPOSIT.
Claim 3
Dependent Claim
33 words
The method according to claim 1 which includes the additional step of freezing the nitroblue tetrazolium dye, water and dried human plasma mixture for storage purposes and thawing same when ready for use.
Claim 4
Dependent Claim
19 words
The method according to claim 1 which includes the additional step of dissolving a Lipopolysaccharide in said distilled water.
Claim 5
Dependent Claim
19 words
The method according to claim 2 which includes the additional step of dissolving a Lipopolysaccharide in said distilled water.
Claim 6
Dependent Claim
29 words
The method according to claim 3 in which said Lipopolysaccharide is chosen from the group containing Lipopolysaccharide E. Coli, Lipopolysaccharide Staphylococcus 17A, Lipopolysaccharide S. Marcescens and Lipopolysaccharide S. Enteritidis.
Claim 7
Dependent Claim
29 words
The method according to claim 4 in which said Lipopolysaccharide is chosen from the group containing Lipopolysaccharide E. Coli, Lipopolysaccharide Staphylococcus 17A, Lipopolysaccharide S. Marcescens and Lipopolysaccharide S. Enteritidis.
Claim 8
Dependent Claim
23 words
A reagent for use in nitroblue tetrazolium tests comprising in combination a mixture of distilled water, nitroblue tetrazolium dye and dried human plasma.
Claim 9
Dependent Claim
23 words
A reagent for use in nitroblue tetrazolium tests comprising in combination a mixture of distilled water, nitroblue tetrazolium dye and dried human plasma.
Claim 10
Dependent Claim
21 words
The reagent according to claim 7 which includes a quantity of Lipopolysaccharide added to said mixture to produce a stimulating reagent.
Claim 11
Dependent Claim
28 words
The reagent according to claim 8 in which the Lipopolysaccharide is chosen from the group including Lipopolysaccharide E. Coli, Lipopolysaccharide Staphylococcus 17A, Lipopolysaccharide S. Marcescens, Lipopolysaccharide S. Enteritidis.
Claim 12
Dependent Claim
21 words
The reagent according to claim 8 in which the concentration of Lipopolysaccharide per test is between 1 microgram and 200 micrograms.
Claim 13
Dependent Claim
21 words
The reagent according to claim 9 in which the concentration of Lipopolysaccharide per test is between 1 microgram and 200 micrograms.
References Cited (Backward Citations)
US3834874A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-22 | 1979-06-23 | 1979-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-22 | 1983-06-23 | 1983-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-22 | 1987-06-23 | 1987-12-23 | $7700.00 | Window Closed / Lapsed |