US3925344A
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Plasma protein substitute
Assignee: New York Blood Center
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Filing: 1974-02-13
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Granted: 1975-12-09
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Expires: 1994-02-13
Abstract
Abstract A plasma protein substitute having polymer repeating units of the formula: WHICH PLASMA PROTEIN SUBSTITUTE HAS AN AVERAGE MOLECULAR WEIGHT BETWEEN 68,000 AND 600,000; AN INTRA-MOLECULAR CROSS-LINKED HEMOGLOBIN COMPOSITION WHEREIN POLYPEPTIDE UNITS OF A HEMOGLOBIN TETRAMER ARE JOINED TOGETHER BY A CROSS-LINKING AGENT HAVING THE RESIDUE: wherein m is 6 to 12, especially an intra-molecular cross-linked hemoglobin composition wherein single polypeptide units are joined together by the cross-linking residue set forth above, and the method of preparing said composition.
Inventors
- 👤 Abraham Mazur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
19 words
A PLASMA PROTEIN SUBSTITUTE COMPRISING A HELOGLOBIN CONTAINING MATERIAL IN POLYMERIC FORM, SAID POLYMER HAVING THE FOLLOWING REPEATING UNIT:
Claim 2
Dependent Claim
19 words
A PLASMA PROTEIN SUBSTITUTE COMPRISING A HELOGLOBIN CONTAINING MATERIAL IN POLYMERIC FORM, SAID POLYMER HAVING THE FOLLOWING REPEATING UNIT:
Claim 3
Dependent Claim
16 words
A composition according to claim 1 wherein said polymer has between 1 and 10 repeating units.
Claim 4
Dependent Claim
13 words
A composition according to claim 1 wherein m is between 1 and 4.
Claim 5
Independent Claim
19 words
A method of preparing a cross-linked hemoglobin material which comprises contacting hemoglobin with a cross-linking agent having the formula:
Claim 6
Independent Claim
19 words
A method of preparing a cross-linked hemoglobin material which comprises contacting hemoglobin with a cross-linking agent having the formula:
Claim 7
Dependent Claim
44 words
A method according to claim 4 wherein the reaction is carried out for a period up to about 2 hours while continuously adding triethylamine to maintain the pH between 7.5 and 8.5, the reaction being carried out at a temperature between 0* and 5*C.
Claim 8
Dependent Claim
43 words
A method according to claim 5 wherein the hemoglobin is present in an amount between 7 and 12 grams per 100 cc of solution being treated, and there is present about 4.4 grams of cross-linking agent for 100 cc. of 10% hemoglobin solution.
Claim 9
Dependent Claim
13 words
A process according to claim 6 wherein m is between 1 and 4.
Claim 10
Dependent Claim
17 words
A process according to claim 7 wherein the cross-linking agent is in the form of a hydrochloride.
Claim 11
Dependent Claim
14 words
A process according to claim 8 wherein the cross-linking agent is diethyl malonimidate hydrochloride.
Claim 12
Dependent Claim
12 words
A process according to claim 4 wherein m is 6 to 12.
Claim 13
Dependent Claim
19 words
A process according to claim 10 wherein the hemoglobin is present as a 0.5 to 8% weight percent solution.
Claim 14
Dependent Claim
19 words
A process according to claim 11 wherein the hemoglobin is present as a 0.5 to 1.5 weight percent solution.
Claim 15
Dependent Claim
13 words
A process according to claim 10 wherein the cross-linking agent is dimethyl suberimidate.
Claim 16
Dependent Claim
13 words
A process according to claim 10 wherein the cross-linking agent is dimethyl azelaimidate.
Claim 17
Dependent Claim
13 words
A process according to claim 10 wherein the cross-linking agent is dimethyl sebacimidate.
Claim 18
Dependent Claim
13 words
A prOcess according to claim 10 wherein the cross-linking agent is dimethyl decanoimidate.
Claim 19
Dependent Claim
13 words
A process according to claim 10 wherein the cross-linking agent is dimethyl dodecanoimidate.
Claim 20
Dependent Claim
13 words
A process according to claim 4 wherein the cross-linking agent is dimethyl adipimidate.
Claim 21
Dependent Claim
31 words
An intramolecular cross-linked hemoglobin composition wherein at least one polypeptide unit thereof is cross-linked to another polypeptide single unit of the same hemoglobin tetramer by a cross-linking agent having the formula
Claim 22
Dependent Claim
31 words
An intramolecular cross-linked hemoglobin composition wherein at least one polypeptide unit thereof is cross-linked to another polypeptide single unit of the same hemoglobin tetramer by a cross-linking agent having the formula
Claim 23
Dependent Claim
22 words
A cross-linking agent according to claim 19 wherein at least 80% of the components have a molecular weight between 68,000 and 85,000.
Claim 24
Dependent Claim
12 words
An intra-molecular cross-linked hemoglobin composition according to claim 20 wherein m 6.
Claim 25
Dependent Claim
12 words
An intramolecular cross-linked hemoglobin composition according to claim 20 wherein m 7.
Claim 26
Dependent Claim
12 words
An intramolecular cross-linked hemoglobin composition according to claim 20 wherein m 8.
References Cited (Backward Citations)
US3788948A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-08 | 1979-06-09 | 1979-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-08 | 1983-06-09 | 1983-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-08 | 1987-06-09 | 1987-12-09 | $7700.00 | Window Closed / Lapsed |