US3925166A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Automated system for the determination of bacterial antibiotic susceptibilities
Assignee: United States Department of Health and Human Services
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Filing: 1974-09-06
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Granted: 1975-12-09
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Expires: 1994-09-06
Abstract
Abstract A system -for automatically determining the sensitivity of a number of different antibiotics to a particular strain of bacteria- comprises an improved sample plate containing a plurality of channels to which differing concentrations and types of antibiotics are introduced. An agar solution containing a suspension of bacteria is then drawn into the different chambers of the sample plate and allowed to gel. The plate is then scanned, both before incubation and then after incubation, by an intense beam of light in order to count the number of bacterial colonies in each channel to determine which antibiotic best combats that particular strain of bacteria. The output of these scans can then be passed to a computer which analyzes the results of the scans and indicates which of the tested antibiotics is best suited for dealing with that particular strain of bacteria.
Inventors
- 👤 Philip Blume
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:33
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/08; C12M23/04; C12M23/20; C12M23/22; C12M23/38; C12M23/40; C12M23/46; C12M41/00; C12M41/46; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
187 words
A SAMPLE PLATE FOR TESTING ANTIBIOTIC SUSCEPTIBILITY OF BACTERIALLY IMPREGNATED GELS COMPRISING: A TANSPARENT PLATE HAVING UPPER AND LOWER SURFACES; A PLURALIRY OF PARALLEL GROOVES IN SAID PLATE EACH OF SAID GROOVES HAVING AN OUTLET END AND AN INLET END; FIRST AND SECOND MANIFILDS DISPOSED IN SAID PLATE RESPECTIVELY AT SAID INLET END AND SAID OUTLET END OF SAID PARALLEL GROOVES; FIRST CONNECTING MEANS FOR CONNECTING EACH OF SAID INLET ENDS OF SAID PARALLEL GROOVES TO SAID FIRST MANIFOLD; SECOND CONNECTING MEANS FOR CONNECTING SAID OUTLET ENDS OF SAID PARALLEL GROOVES TO SAID SECOND MANIFOLD; AN INLET BORE CONNECTED TO SAID FIRST MANIFOLD THROUGH WHICH A SOLUTION OF AGAR MAY BE INTRODUCED TO SAID FIRST MANIFOLD; AN ASPIRATION BORE CONNECTED TO SAID SECOND MANIFOLD, SAID ASPIRATION BORE BEING ADAPTED TO BE CONNECTED TO A MEANS FOR APPLYING A SUCTION TO SAID ASPIRATION BORE, AND A TRANSPARENT COVERING MEANS, SEALINGLY ENGAGING SAID UPPER SURFACE OF SAID TRANSPARENT PLATE, FOR FORMING, WITH SAID FIRST AND SECOND CONNECTING MEANS AND SAID PARALLEL GROOVES, A PLURALITY OF SEALED TUBE-LIKE CHANNELS CONNECTING SAID FIRST AND SECOND MANIFOLDS, AND FOR SEALING SAID MANIFOLDS.
Claim 2
Dependent Claim
187 words
A SAMPLE PLATE FOR TESTING ANTIBIOTIC SUSCEPTIBILITY OF BACTERIALLY IMPREGNATED GELS COMPRISING: A TANSPARENT PLATE HAVING UPPER AND LOWER SURFACES; A PLURALIRY OF PARALLEL GROOVES IN SAID PLATE EACH OF SAID GROOVES HAVING AN OUTLET END AND AN INLET END; FIRST AND SECOND MANIFILDS DISPOSED IN SAID PLATE RESPECTIVELY AT SAID INLET END AND SAID OUTLET END OF SAID PARALLEL GROOVES; FIRST CONNECTING MEANS FOR CONNECTING EACH OF SAID INLET ENDS OF SAID PARALLEL GROOVES TO SAID FIRST MANIFOLD; SECOND CONNECTING MEANS FOR CONNECTING SAID OUTLET ENDS OF SAID PARALLEL GROOVES TO SAID SECOND MANIFOLD; AN INLET BORE CONNECTED TO SAID FIRST MANIFOLD THROUGH WHICH A SOLUTION OF AGAR MAY BE INTRODUCED TO SAID FIRST MANIFOLD; AN ASPIRATION BORE CONNECTED TO SAID SECOND MANIFOLD, SAID ASPIRATION BORE BEING ADAPTED TO BE CONNECTED TO A MEANS FOR APPLYING A SUCTION TO SAID ASPIRATION BORE, AND A TRANSPARENT COVERING MEANS, SEALINGLY ENGAGING SAID UPPER SURFACE OF SAID TRANSPARENT PLATE, FOR FORMING, WITH SAID FIRST AND SECOND CONNECTING MEANS AND SAID PARALLEL GROOVES, A PLURALITY OF SEALED TUBE-LIKE CHANNELS CONNECTING SAID FIRST AND SECOND MANIFOLDS, AND FOR SEALING SAID MANIFOLDS.
Claim 3
Dependent Claim
53 words
The sample plate of claim 1, wherein said means for connecting said parallel grooves to said first manifold Comprise first tubular constrictions narrower in width than said parallel grooves; and said means for connecting said parallel grooves to said second manifold comprise second tubular constrictions narrower in width than said first tubular constrictions.
Claim 4
Dependent Claim
17 words
The sample plate of claim 1, further comprising antibiotic containing porous strips disposed in said parallel grooves.
Claim 5
Dependent Claim
24 words
The device of claim 3, wherein said porous strips comprise antibiotic impregnated paper having an adhesive adhering to the sides of said parallel grooves.
Claim 6
Dependent Claim
21 words
The sample plate of claim 1, wherein said transparent covering comprises a sheet of clear plastic adhexively mounted on said plate.
Claim 7
Dependent Claim
15 words
A device in accordance with claim 5, wherein said clear sheet is an acrylic plate.
Claim 8
Dependent Claim
15 words
A device in accordance with claim 5, wherein said clear sheet is a plastic film.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-08 | 1979-06-09 | 1979-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-08 | 1983-06-09 | 1983-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-08 | 1987-06-09 | 1987-12-09 | $7700.00 | Window Closed / Lapsed |