US3922194A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing 2-keto-L-gulonic acid
Assignee: Shionogi and Co Ltd
•
Filing: 1974-03-19
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Granted: 1975-11-25
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Expires: 1994-03-19
Abstract
Abstract 2-Keto-L-gulonic acid is prepared from 2,5-diketo-D-gluconic acid through microbial conversion. The 2-keto-L-gulonic acid producing microorganism used for this microbial conversion includes strains which belong to genera of Brevibacterium, Arthrobacter, Micrococcus, Staphylococcus, Pseudomonas and Bacillus. Both the incubation of the microorganism in a medium containing 2,5-diketo-L-gluconic acid and the direct contact of any products obtained from the cells with a substrate containing said 2,5-diketo-D-gluconic acid may be used in the disclosed process.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Bunji Kageyama
- 👤 Tahiko Honjo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 04:38
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; C07; C07C; C07C59/00; C07C59/185; C07C59/215; C07H; C07H7/00; C07H7/02; C07H7/027; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/832; Y10S435/837; Y10S435/84; Y10S435/859; Y10S435/874; Y10S435/882; Y10S435/883
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
62 words
A PROCESS FOR PRODUCING 2-KETO-L-GULONIC ACID OR A SALT THEREOF WHICH COMPRISES; CONTACTING A 2-KETO-L-GULONIC ACID PRODUCING STRAIN SELECTED FROM MIMICROORGANISMS WHICH BELONG TO THE GENERA OF BREVIBACTERIUM, ARTHROBACTER, BACILLUS, STAPHYLOCOCCUS, MICROCOCCUS AND PSEUDOMONAS, WITH 2,5DIKETO-D-GLUCONIC ACID OR A SALT THEREOF, UNDER CONDITIONS SUFFICIENT TO PRODUCE 2-KETO-L-GULONIC ACID, AND RECOVERING THE PRODUCED 2-KETO-L-GULONIC ACID OR ANY SALTS THEREOF FROM THE RESULTANT MIXTURE.
Claim 2
Dependent Claim
62 words
A PROCESS FOR PRODUCING 2-KETO-L-GULONIC ACID OR A SALT THEREOF WHICH COMPRISES; CONTACTING A 2-KETO-L-GULONIC ACID PRODUCING STRAIN SELECTED FROM MIMICROORGANISMS WHICH BELONG TO THE GENERA OF BREVIBACTERIUM, ARTHROBACTER, BACILLUS, STAPHYLOCOCCUS, MICROCOCCUS AND PSEUDOMONAS, WITH 2,5DIKETO-D-GLUCONIC ACID OR A SALT THEREOF, UNDER CONDITIONS SUFFICIENT TO PRODUCE 2-KETO-L-GULONIC ACID, AND RECOVERING THE PRODUCED 2-KETO-L-GULONIC ACID OR ANY SALTS THEREOF FROM THE RESULTANT MIXTURE.
Claim 3
Dependent Claim
46 words
A process as claimed in claim 1, wherein the 2-keto-L-gulonic acid producing strain is incubated in a medium containing 2,5-diketo-D-gluconic acid or a salt thereof to effect a fermentative action therein, and recovering 2-keto-L-gulonic acid or any salts thereof accumulated during the incubation from the medium.
Claim 4
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Brevibacterium.
Claim 5
Dependent Claim
14 words
A process as claimed in claim 3, wherein said strain belongs to Brevibacterium ketosoreductum.
Claim 6
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Artheofacter.
Claim 7
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Bacillus.
Claim 8
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Staphylococcus.
Claim 9
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Micrococcus.
Claim 10
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid pRoducing strain belongs to the genus of Pseudomonas.
Claim 11
Dependent Claim
56 words
A process as claimed in claim 1, wherein cells of said microorganism strain are contacted with a substance containing 2, 5-diketo-D-gluconic acid or a salt thereof under conditions sufficient to produce 2-keto-L-gulonic acid, and the 2-keto-L-gulonic acid or any salts thereof produced and accumulated as a result of the contact are recovered from the resultant mixture.
Claim 12
Dependent Claim
29 words
A process as claimed in claim 1, wherein an aqueous medium containing viable cells of said microorganism is contacted with a substance containing 2,5-diketo-D-gluconic acid or a salt thereof.
Claim 13
Dependent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises cultivating 2-keto-L-gulonic acid producing microorganisms selected from the group consisting of Brevibacterium ketosoreductum, Arthrobacter simplex, Bacillus megaterium, Staphylococcus aureus, Micrococcus dinitrificans, Micrococcus rubens, Micrococcus roseus and Pseudomonas chlororaphis, and mutants thereof, in an aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or salts thereof from the resultant mixture.
Claim 14
Dependent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises cultivating 2-keto-L-gulonic acid producing microorganisms selected from the group consisting of Brevibacterium ketosoreductum, Arthrobacter simplex, Bacillus megaterium, Staphylococcus aureus, Micrococcus dinitrificans, Micrococcus rubens, Micrococcus roseus and Pseudomonas chlororaphis, and mutants thereof, in an aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or salts thereof from the resultant mixture.
Claim 15
Dependent Claim
15 words
A process as claimed in claim 12, wherein said microorganism is Brevibacterium ketosoreductum ATCC 21914.
Claim 16
Dependent Claim
15 words
A process as claimed in claim 12, wherein said microorganism is Arthrobacter simplex ATCC 21917.
Claim 17
Dependent Claim
15 words
A process as claimed in claim 12, wherein said microorganism is Bacillus megaterium ATCC 21916.
Claim 18
Dependent Claim
15 words
A process as claimed in claim 12, wherein said microorganism is Staphylococcus aureus ATCC 21915.
References Cited (Backward Citations)
US2741577A
Cited
US3076750A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-24 | 1979-05-26 | 1979-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-24 | 1983-05-26 | 1983-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-24 | 1987-05-26 | 1987-11-25 | $7700.00 | Window Closed / Lapsed |