US3919082A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Elution apparatus and method
Assignee: Sandoz AG
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Filing: 1974-03-07
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Granted: 1975-11-11
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Expires: 1994-03-07
Abstract
Abstract The invention concerns an elution apparatus and method. The elution apparatus disclosed is suitable for eluting a chromatographically isolated substance directly from the absorption layer of a thin layer chromatographic plate.
Inventors
- 👤 Heinz Falk
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 18:38
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/90; G01N30/94
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
184 words
AN ELUTION APPARATUS FOR ELUTING A CHROMATOGRAPHICALLY ISOLATED SUBSTANCE FROM THE ABSORPTION LAYER OF A THIN LAYER CHROMATOGRAPHIC PLATE, WHICH COMPRISES AN ELUTION HEAD HAVING A BASE SECTION WHICH HAS A FLAT BOTTOM SURFACE, A SEALING SKIRT ABOUT THE BASE SECTION STANDING PROUD OF SAID BOTTOM SURFACE TO THE EXTENT OF ONLY SLIGHTLY GREATER THAN THE THICKNESS OF THE ABSORPTION LAYER TO PROVIDE A SEAL BETWEEN THE BASE SECTION AND SURFACE OF THE CHROMATOGRAPHIC PLATE CLEARED OF ABSORPTION LAYER AROUND THE ISOLATED SUBSTANCE AND TO THUS DEFINE AN ELUTION CHAMBER, AN ELUENT LEADING INTO THE ELUTION CHAMBER AT A POSITION ADJACENT THE SEALING SKIRT TO ONE SAID OF THE ELUTION CHAMBER, AN ELUATE OUTLET CONDUIT LEADING FROM THE ELUTION CHAMBER AT A POSITION ADJACENT THE SEALING SKIRT TO THE OPPOSING SIDE OF THE ELUTION CHAMBER, CONNECTION MEANS FOR CONNECTING UP THE INLET AND OUTLET CONDUITS RESPECTIVELY TO AN ELUENT SUPPLY LINE AND ELUATE TAKE OFF LINE, A TILTABLE TABLE TO SUPPORT THE THIN LAYER CHROMATOGRPHIC PLATE, AND CLAMP MEANS ASSOCIATED WITH THE TABLE TO CLAMP THE ELUTION HEAD IN POSITION ON THE CHROMATOGRAPHIC PLATE.
Claim 2
Dependent Claim
184 words
AN ELUTION APPARATUS FOR ELUTING A CHROMATOGRAPHICALLY ISOLATED SUBSTANCE FROM THE ABSORPTION LAYER OF A THIN LAYER CHROMATOGRAPHIC PLATE, WHICH COMPRISES AN ELUTION HEAD HAVING A BASE SECTION WHICH HAS A FLAT BOTTOM SURFACE, A SEALING SKIRT ABOUT THE BASE SECTION STANDING PROUD OF SAID BOTTOM SURFACE TO THE EXTENT OF ONLY SLIGHTLY GREATER THAN THE THICKNESS OF THE ABSORPTION LAYER TO PROVIDE A SEAL BETWEEN THE BASE SECTION AND SURFACE OF THE CHROMATOGRAPHIC PLATE CLEARED OF ABSORPTION LAYER AROUND THE ISOLATED SUBSTANCE AND TO THUS DEFINE AN ELUTION CHAMBER, AN ELUENT LEADING INTO THE ELUTION CHAMBER AT A POSITION ADJACENT THE SEALING SKIRT TO ONE SAID OF THE ELUTION CHAMBER, AN ELUATE OUTLET CONDUIT LEADING FROM THE ELUTION CHAMBER AT A POSITION ADJACENT THE SEALING SKIRT TO THE OPPOSING SIDE OF THE ELUTION CHAMBER, CONNECTION MEANS FOR CONNECTING UP THE INLET AND OUTLET CONDUITS RESPECTIVELY TO AN ELUENT SUPPLY LINE AND ELUATE TAKE OFF LINE, A TILTABLE TABLE TO SUPPORT THE THIN LAYER CHROMATOGRPHIC PLATE, AND CLAMP MEANS ASSOCIATED WITH THE TABLE TO CLAMP THE ELUTION HEAD IN POSITION ON THE CHROMATOGRAPHIC PLATE.
Claim 3
Dependent Claim
77 words
An elution apparatus according to claim 1, in which the clamp means comprises a lever arm pivotally mounted to the table, one free end of the lever arm to one side of the pivot being for engagement with the elution head and the other free end of the lever arm to the other side of the pivot having adjustment means associated with the table to adjust the clamping action of the lever arm on the elution head.
Claim 4
Dependent Claim
46 words
An elution apparatus according to claim 1, in which the table is tiltable between a horizontal position and an inclined position substantially parallel to an eluent supply line and eluate take off line leading downwardly via the inlet and outlet conduits to an eluant collection point.
Claim 5
Dependent Claim
61 words
An elution apparatus according to claim 1, in which the connection means for connecting up the inlet and outlet conduits respectively to an eluent supply line and eluate take off line comprises threaded bores communicating with the inlet and outlet conduits to receive correspondingly threaded hollow screws provided at the connection ends of the eluent supply and eluate take off lines.
Claim 6
Dependent Claim
88 words
Method of eluting a chromatographically isolated substance in situ from the absorption layer of a thin layer chromatographic plate, which comprises clearing the surface of the chromatographic plate of absorption layer about the isolated substance, confining the absorption layer carrying said isolated substance in situ on the chromatographic plate in a volume which is substantially filled by the volume of the absorption layer carrying the isolated substance, and eluting the isolated substance from the enclosed absorption layer by passing eluent through said confined volume and collecting the eluate.
Claim 7
Dependent Claim
88 words
Method of eluting a chromatographically isolated substance in situ from the absorption layer of a thin layer chromatographic plate, which comprises clearing the surface of the chromatographic plate of absorption layer about the isolated substance, confining the absorption layer carrying said isolated substance in situ on the chromatographic plate in a volume which is substantially filled by the volume of the absorption layer carrying the isolated substance, and eluting the isolated substance from the enclosed absorption layer by passing eluent through said confined volume and collecting the eluate.
References Cited (Backward Citations)
US3503712A
Cited
US3477950A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-10 | 1979-05-12 | 1979-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-10 | 1983-05-12 | 1983-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-10 | 1987-05-12 | 1987-11-11 | $7700.00 | Window Closed / Lapsed |