US3917515A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Serum lipase method and medium
Assignee: Individual
•
Filing: 1974-03-13
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Granted: 1975-11-04
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Expires: 1994-03-13
Abstract
Abstract Method and medium for the measurement of pancreatic lipase activity in serum. The method involves contacting a serum lipase containing fluid with a medium which enables differential diffusion of the lipase and other constituents in the fluid to take place to provide diagnostically meaningful indicia of lipase activity in the medium. The medium comprises a substrate containing a substance capable of being acted upon by pancreatic lipase, and an agent for inhibiting or impeding the activity of competing enzymes in serum. The substrate has a pH in a range which favors pancreatic lipase activity, and is supported in a normally nonfluid, inert material which favors differential diffusion of the pancreatic lipase. An antiseptic, which may be a bactericide or microbicide, advantageously is incorporated into the substrate to prevent bacterial growth during incubation of the medium in testing for lipase activity, and storage.
Inventors
- 👤 Jack M Goldberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 17:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
130 words
A METHOD OF MEASURING PANCREATIC LIPASE ACTIVITY IN SERUM COMPRISING: PROVIDING A MEDIUM COMPRISING A SUBSTANTIALLY INERT, SUPPORTING MATRIX HAVING A POROSITY WHICH ENABLES PANCREATIC LIPASE TO DIFFUSE TEREIN AT A RATE WHICH IS DIFFERENT FROM THE RATE AT WHICH LIPASE CONPETING AGENTS IN SERUM WILL DIFFUSE THEREIN SAID MEDIUM CONTAINING A SUBSTANCE CAPABLE OF BEING ACTED UPON BY THE PANCREATIC LIPASE AND A SUBSTANCE WHICH ACTS TO INHIBIT THE ACTIVITY OF PANCREATIC LIPASE COMPETING AGENTS PRESENT IN SERUM, INTRODUCING INTO THE MEDIUM SERUM CONTAINING PANCREATIC LIPASE, AND ALLOWING THE PANCREATIC LIPASE AND ANY PANCREATIC LIPASE COMPETING AGENTS PRESENT IN THE SERUM TO DIFFERENTIALLY DIFFUSE IN THE MEDIUM AND THE PANCREATIC LIPASE TO ACT UPON SAID FIRST MENTIONED SUBSTANCE TO PROVODE IN THE MEDIUM MEASURABLE INDICIA OF PANCREATIC LIPASE ACTIVITY.
Claim 2
Independent Claim
130 words
A METHOD OF MEASURING PANCREATIC LIPASE ACTIVITY IN SERUM COMPRISING: PROVIDING A MEDIUM COMPRISING A SUBSTANTIALLY INERT, SUPPORTING MATRIX HAVING A POROSITY WHICH ENABLES PANCREATIC LIPASE TO DIFFUSE TEREIN AT A RATE WHICH IS DIFFERENT FROM THE RATE AT WHICH LIPASE CONPETING AGENTS IN SERUM WILL DIFFUSE THEREIN SAID MEDIUM CONTAINING A SUBSTANCE CAPABLE OF BEING ACTED UPON BY THE PANCREATIC LIPASE AND A SUBSTANCE WHICH ACTS TO INHIBIT THE ACTIVITY OF PANCREATIC LIPASE COMPETING AGENTS PRESENT IN SERUM, INTRODUCING INTO THE MEDIUM SERUM CONTAINING PANCREATIC LIPASE, AND ALLOWING THE PANCREATIC LIPASE AND ANY PANCREATIC LIPASE COMPETING AGENTS PRESENT IN THE SERUM TO DIFFERENTIALLY DIFFUSE IN THE MEDIUM AND THE PANCREATIC LIPASE TO ACT UPON SAID FIRST MENTIONED SUBSTANCE TO PROVODE IN THE MEDIUM MEASURABLE INDICIA OF PANCREATIC LIPASE ACTIVITY.
Claim 3
Dependent Claim
33 words
A method according to claim 1 wherein the medium enables the pancreatic lipase to diffuse at a rate which is greater than the rate at which lipase competing agents in serum will diffuse.
Claim 4
Dependent Claim
19 words
A method according to claim 1 wherein the medium is incubated at a temperature of about 30*C during diffusion.
Claim 5
Dependent Claim
64 words
A method according to claim 1 wherein a depression is formed in the medium for receiving the pancreatic lipase containing serum and diffusion of the lipase takes place outwardly and radially from the depression, the action of the lipase upon said substance taking place in a circular pattern visible in the medium which pattern provides a measure of pancreatic lipase activity in the serum.
Claim 6
Dependent Claim
50 words
A method according to claim 1 wherein the matrix is formed of a member of the group consisting of agar, agarose and polyacrylamide, and the substance upon which the lipase can act is a triester of a polyhydric alcohol wherein the acyl groups contain from 11 to 32 carbon atoms.
Claim 7
Independent Claim
81 words
A medium for use in the measurement of pancreatic lipase activity in serum comprising a substrate containing a substance capable of being acted upon by pancreatic lipase and an agent for impeding the activity of pancreatic lipase competing agents present in serum, said substrate being supported in a substantially inert matrix having a porosity which enables pancreatic lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein.
Claim 8
Independent Claim
81 words
A medium for use in the measurement of pancreatic lipase activity in serum comprising a substrate containing a substance capable of being acted upon by pancreatic lipase and an agent for impeding the activity of pancreatic lipase competing agents present in serum, said substrate being supported in a substantially inert matrix having a porosity which enables pancreatic lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein.
Claim 9
Dependent Claim
18 words
A medium according to claim 6 wherein the substance upon which the lipase can act is a triglyceride.
Claim 10
Dependent Claim
19 words
A medium according to claim 7 wherein the triglyceride is a triglyceride of a higher molecular weight fatty acid.
Claim 11
Dependent Claim
17 words
A medium according to claim 8 wherein the fatty acid contains from 11 to 32 carbon atoms.
Claim 12
Dependent Claim
18 words
A medium according to claim 6 wherein the substance upon which the lipase can act is olive oil.
Claim 13
Dependent Claim
24 words
A medium according to claim 6 wherein the agent for impeding The activity of lipase competing agents present in serum comprises a bile salt.
Claim 14
Dependent Claim
16 words
A medium according to claim 11 wherein the bile salt is sodium deoxycholate or sodium taurocholate.
Claim 15
Dependent Claim
22 words
A medium according to claim 6 wherein the substrate is maintained at a pH in a range which favors pancreatic lipase activity.
Claim 16
Dependent Claim
22 words
A medium according to claim 13 wherein the pH of the substrate is in the range of about 8 to about 10.
Claim 17
Dependent Claim
29 words
A medium according to claim 6 wherein a buffer is incorporated into the medium to maintain the pH of the medium in a range which favors pancreatic lipase activity.
Claim 18
Dependent Claim
14 words
A medium according to claim 15 wherein the buffer is tris (hydroxymethyl) aminomethane/hydrochloric acid.
Claim 19
Dependent Claim
16 words
A medium according to claim 6 wherein a microbicide is incorporated therein to inhibit bacterial growth.
Claim 20
Dependent Claim
12 words
A medium according to claim 17 wherein the microbicide is sodium azide.
Claim 21
Independent Claim
84 words
A medium for use in the measurement of pancreatic lipase activity in serum comprising a substrate containing olive oil, a bile salt, a microbicide, and a buffer for maintaining the pH of the substrate in the range of from about 8 to about 10, said substrate being supported in a substantially inert matrix having a porosity which enables tha pancreatic lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein.
Claim 22
Independent Claim
84 words
A medium for use in the measurement of pancreatic lipase activity in serum comprising a substrate containing olive oil, a bile salt, a microbicide, and a buffer for maintaining the pH of the substrate in the range of from about 8 to about 10, said substrate being supported in a substantially inert matrix having a porosity which enables tha pancreatic lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein.
Claim 23
Dependent Claim
15 words
A medium according to claim 19 wherein the substantially inert matrix is agar or agarose.
Claim 24
Independent Claim
130 words
A method of preparing a medium for the measurement of pancreatic lipase activity in serum comprising: providing a substrate containing as essential ingredients a substance capable of being acted upon by pancreatic lipase and an agent for impeding the activity of pancreatic lipase competing agents present in serum, forming an intimate mixture of the substrate and a matrix forming material for supporting the substrate, said material being characterized in that it is substantially inert and provides a matrix having a porosity which enables the lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein, whereby the lipase will be able to act upon said substance to provide measurable indicia of pancreatic lipase activity in the medium.
Claim 25
Independent Claim
130 words
A method of preparing a medium for the measurement of pancreatic lipase activity in serum comprising: providing a substrate containing as essential ingredients a substance capable of being acted upon by pancreatic lipase and an agent for impeding the activity of pancreatic lipase competing agents present in serum, forming an intimate mixture of the substrate and a matrix forming material for supporting the substrate, said material being characterized in that it is substantially inert and provides a matrix having a porosity which enables the lipase to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein, whereby the lipase will be able to act upon said substance to provide measurable indicia of pancreatic lipase activity in the medium.
Claim 26
Dependent Claim
33 words
A method according to claim 21 wherein the substrate is formed into a stable emulsion and is admixed with the matrix forming material in a manner to maintain it in an emulsified condition.
Claim 27
Dependent Claim
45 words
A method according to claim 21 wherein the substance which is capable of being acted upon by pancreatic lipase is a mixture of fatty acid triglycerides, and the agent for impeding the activity of pancreatic lipase competing agents present in serum is a bile salt.
Claim 28
Dependent Claim
27 words
A method according to claim 21 wherein the substance capable of being acted upon by pancreatic lipase is olive oil which is formed into a stable emulsion.
Claim 29
Dependent Claim
62 words
A method according to claim 21 wherein the substrate is formed into a stable emulsion containing olive oil, a bile salt, a microbicide and a buffer, said emulsion being admixed with a matrix forming material comprising a member selected from the group consisting of agar and agarose in a manner to maintain it in an emulsified condition in the matrix forming material.
Claim 30
Dependent Claim
82 words
An article for use in making determinations of pancreatic lipase activity in serum comprising a body portion having a layer of a medium therein for receiving serum containing pancreatic lipase, said medium comprising a substantially inert, supporting matrix having a porosity which enables the lipase in serum to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein and containing a substance capable of being acted upon by the lipase.
Claim 31
Dependent Claim
82 words
An article for use in making determinations of pancreatic lipase activity in serum comprising a body portion having a layer of a medium therein for receiving serum containing pancreatic lipase, said medium comprising a substantially inert, supporting matrix having a porosity which enables the lipase in serum to diffuse therein at a rate which is different from the rate at which lipase competing agents present in serum will diffuse therein and containing a substance capable of being acted upon by the lipase.
Claim 32
Dependent Claim
21 words
An article according to claim 26 wherein the body portion comprises a shallow, open-faced container, and the medium is essentially solid.
Claim 33
Dependent Claim
45 words
An aRticle according to claim 26 wherein the medium comprises a substrate which is supported in the matrix, the substrate being in the form of an emulsion which is stabilized in the matrix, the medium being maintained at a pH which favors pancreatic lipase activity.
Claim 34
Dependent Claim
24 words
An article according to claim 28 wherein the substrate comprises a mixture of fatty acid triglycerides, a bile salt, a microbicide and a buffer.
Claim 35
Dependent Claim
24 words
An article according to claim 26 wherein the substance capable of being acted upon by the lipase comprises a stabilized emulsion of olive oil.
References Cited (Backward Citations)
US3689364A
Cited
US3778352A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-03 | 1979-05-05 | 1979-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-03 | 1983-05-05 | 1983-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-03 | 1987-05-05 | 1987-11-04 | $7700.00 | Window Closed / Lapsed |