US3917510A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Lysis of yeast cell walls
Assignee: Kirin Brewery Co Ltd
•
Filing: 1974-04-08
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Granted: 1975-11-04
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Expires: 1994-04-08
Abstract
Abstract Lysis of yeast cell walls in an aqueous medium by an enzyme which is capable of lysing the yeast cell walls is accelerated by effecting the lysis in the presence of a water-soluble sulfite or by effecting the lysis on the yeast which has been pretreated with a water-soluble sulfite. The water-soluble sulfite can contain another water-soluble inorganic salt or cupric ions.
Inventors
- 👤 Kumpei Kitamura
- 👤 Yasushi Yamamoto
- 👤 Eiichi Kokubo
- 👤 Yoshiro Kuroiwa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 04:51
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; C12N1/063; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/911; Y10S435/921; Y10S435/923; Y10S435/94; Y10S435/941; Y10S435/942; Y10S435/944
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
103 words
IN A METHOD OF LYSIS OF YEAST CELL WALL WHEREIN THE YEAST IS SUBJECTED TO TREATMENT IN AN AQUEOUS MEDIUM WITH ENZYME WHIC SELECTED FROM THE GROUP CONSISTING OF SNAIL ENZYME AND AN ENZYME PRODUCED BY ARTHROBACTER LETEUS THEREBY TO LYSE THE YEAST CELL WALLS, THE IMPROVEMENT WHICH COMPRISES EFFECTING THE TREATMENT ON THE YEAST CELL WALLS UNDER THE INFLUENCE OF A WATER-SOLUBLE SULFITE WHEREBY THE LYSIS IS ACCELERATED BY THE WATER-SOLUBLE SULFITE, THE INFLUENCE BEING EXERTED BEFORE OR AT THE TIME OF THE TREATMENT BY CONTACTING THE YEAST WITH AN AQUEOUS SULFITE IN A MOLAR CONCENTRATION OF FROM 0.01 TO 1.0 M.
Claim 2
Dependent Claim
103 words
IN A METHOD OF LYSIS OF YEAST CELL WALL WHEREIN THE YEAST IS SUBJECTED TO TREATMENT IN AN AQUEOUS MEDIUM WITH ENZYME WHIC SELECTED FROM THE GROUP CONSISTING OF SNAIL ENZYME AND AN ENZYME PRODUCED BY ARTHROBACTER LETEUS THEREBY TO LYSE THE YEAST CELL WALLS, THE IMPROVEMENT WHICH COMPRISES EFFECTING THE TREATMENT ON THE YEAST CELL WALLS UNDER THE INFLUENCE OF A WATER-SOLUBLE SULFITE WHEREBY THE LYSIS IS ACCELERATED BY THE WATER-SOLUBLE SULFITE, THE INFLUENCE BEING EXERTED BEFORE OR AT THE TIME OF THE TREATMENT BY CONTACTING THE YEAST WITH AN AQUEOUS SULFITE IN A MOLAR CONCENTRATION OF FROM 0.01 TO 1.0 M.
Claim 3
Dependent Claim
34 words
A process for lysis of yeast cell walls as claimed in claim 1 in which said treatment is effected in the presence of said water-soluble sulfite in said molar concentration in said aqueous medium.
Claim 4
Dependent Claim
43 words
A process for lysis of yeast cell walls as claimed in claim 1 in which said treatment is effected after the yeast has been subjected to pretreatment in an aqueous medium with said water-soluble sulfite in said molar concentration in the aqueous medium.
Claim 5
Dependent Claim
36 words
A process for lysis of yeast cell walls as claimed in claim 3 in which said pretreatment comprises suspending said yeast for at least for 0.2 hour in an aqueous medium which contains said water-soluble sulfite.
Claim 6
Dependent Claim
45 words
A process for lysis of yeast cell walls as claimed in claim 1 in which said treatment is effected in the presnece of a water-soluble inorganic salt other than said water-soluble sulfite in a molar concentration in said aqueous medium of up to 3.0 M.
Claim 7
Dependent Claim
50 words
A process for lysis of yeast cell walls as claimed in claim 3 in which said pretreatment in an aqueous medium with said water-soluble sulfite is effected further in the presence of cupric ions in a quantity of millimolar concentration in said aqueous medium of from 0.1 to 20 mM.
Claim 8
Dependent Claim
35 words
A process for lysis of yeast cell walls as claimed in claims 1 in which said water-soluble sulfite is selected from the group consisting of alkali metal sulfites, alkaline earth metal sulfites and ammonium sulfite.
Claim 9
Dependent Claim
55 words
A process for lysis of yeast cell walls as claimed in claim 5 in which said water-soluble inorganic salt other than said water-soluble sulfite is selected from the group consisting of alkali metal salts, alkaline earth metal salts and ammonium salts of hydrochloric acid, sulfuric acid, nitric acid, carbonic acid, bicarbonic acid and phosphoric acid.
Claim 10
Dependent Claim
29 words
A process for lysis of yeast cell walls as claimed in claim 6 in which said cupric ions are provided by cupric sulfate, cupric nitrate and cupric annonium hydroxide.
Claim 11
Dependent Claim
38 words
A process for lysis of yeast cell walls as claimed in claim 2 in which said treatment is effected in the presence of said water-soluble sulfite at a temperature from 15* to 40*C for at least 0.5 hour.
Claim 12
Dependent Claim
36 words
A process foR lysis of yeast cell walls as claimed in claim 10 in which said yeast is dispersed in said aqueous medium in a concentration of from 0.1 to 20% by weight of dry basis.
Claim 13
Dependent Claim
32 words
A process for lysis of yeast cell walls as claimed in claim 3 in which said water-soluble sulfite is in a molar concentration of from 0.01 to 0.5M in said aqueous medium.
Claim 14
Dependent Claim
21 words
A process for lysis of yeast cell walls as claimed in claim 7 in which said water-soluble sulfite is sodium sulfite.
Claim 15
Dependent Claim
45 words
A process for lysis of yeast cell walls as claimed in claim 8 in which said water-soluble inorganic salt is selected from the group consisting of potassium chloride, sodium chloride, ammonium chloride, calcium chloride, sodium nitrate, sodium carbonate, potassium sulfate, ammonium sulfate, and magnesium sulfate.
Claim 16
Dependent Claim
31 words
A process for lysis of yeast cell walls as claimed in claim 14 in which said water-soluble inorganic salt is selected from the group consisting of potassium chloride and sodium chloride.
Claim 17
Dependent Claim
37 words
A process for lysis of yeast cell walls as claimed in claim 1 in which said yeast is selected from the group consisting of those belonging to Saccharomyces, Endomycopsis, Saccharomycodes, Nematospora, Candida, Torulopsis, Brittanumyces, Schizosaccharomyces and Kloekera.
Claim 18
Dependent Claim
31 words
A process for lysis of yeast cell walls as claimed in claim 16 in which said yeast is selected from the group consisting of those belonging to Saccharomyces, Candida and Kloeckera.
Claim 19
Dependent Claim
36 words
A process for lysis of yeast cell walls as claimed in claim 17 in which said yeast is selected from the group consisting of those belonging to Saccharomyces Cerevisiae, Saccharomyces Carlsbergensis, Candida Lipolytica and Kloeckera Japonicum.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-03 | 1979-05-05 | 1979-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-03 | 1983-05-05 | 1983-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-03 | 1987-05-05 | 1987-11-04 | $7700.00 | Window Closed / Lapsed |