US3915856A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of carrying out preparative thin-layer chromatography and apparatus for use in the method
Assignee: Individual
•
Filing: 1974-12-20
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Granted: 1975-10-28
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Expires: 1994-12-20
Abstract
Abstract A method of carrying out preparative thin-layer chromatography comprising application of a layer of adsorbent material to a base plate, application of a sample in the form of a narrow strip in the adsorbent layer and immersion of the base plate in a solvent for the development of the chromatogram is disclosed wherein the sample is applied in solid form, the sample being dispensed in a groove in the adsorbent layer to form a strip which is then compressed or compacted. The invention also comprises apparatus for carrying out the method according to the invention which apparatus includes (a) a bridge member, (b) a holder for cutting blades, (c) an applicator and (d) a press of T-shaped crosssection.
Inventors
- 👤 Holger Meyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 10:44
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/90; G01N30/92; G01N30/93; G01N30/91
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
79 words
A METHOD OF CARRYING OUT PREPARATIVE THIN-LAYER CROMATOGRAPHY COMPRISING APPLICATION OF AN ADSORBENT LAYER TO A BASE PLATE, APPLICATION OF A SAMPLE IN THE FORM OF A NARROW STRIP IN THE ADSORBENT LAYER AND IMMERSION OF THE BASE PLATE IN A SOLVENT FOR THE DEVELOPMENT OF THE CROMATOGRAM, CHARACTERIZED IN THAT TH SAMPLE IS APPLIED IN SOLID FORM, THE SAMPLE BEING DISPENSED IN A GROOVE IN THE ADSORBENT LAYER TO FORM A STRIP WHICH IS THEN COMPRESED OR COMPACTED.
Claim 2
Independent Claim
79 words
A METHOD OF CARRYING OUT PREPARATIVE THIN-LAYER CROMATOGRAPHY COMPRISING APPLICATION OF AN ADSORBENT LAYER TO A BASE PLATE, APPLICATION OF A SAMPLE IN THE FORM OF A NARROW STRIP IN THE ADSORBENT LAYER AND IMMERSION OF THE BASE PLATE IN A SOLVENT FOR THE DEVELOPMENT OF THE CROMATOGRAM, CHARACTERIZED IN THAT TH SAMPLE IS APPLIED IN SOLID FORM, THE SAMPLE BEING DISPENSED IN A GROOVE IN THE ADSORBENT LAYER TO FORM A STRIP WHICH IS THEN COMPRESED OR COMPACTED.
Claim 3
Dependent Claim
19 words
A method according to claim 1, wherein the sample is applied alone or in homogeneous admixture with adsorbent material.
Claim 4
Dependent Claim
48 words
A method according to claim 1, wherein the strip of sample and those parts of the adsorbent layer which, on immersion of the ground plate in the solvent, are adjacent to or below the sample strip are held against the ground plate during the development of the chromatogram.
Claim 5
Dependent Claim
68 words
A method according to claim 3, wherein a sheet of a porous material which does not contaminate the sample, the adsorbent lAyer or the solvent is folded round the adsorbent layer at the edge thereof that is nearest to the strip of sample, care being taken that the sheet covers the strip of sample, and the sheet is secured in this position during the developing of the chromatogram.
Claim 6
Dependent Claim
112 words
A method according to claim 3, wherein the base plate with the adsorbent layer applied thereon is caused to rest upon a plurality of points of support at a small distance from the bottom of a trough having vertical side and end walls and is secured in close contact with one of the vertical side walls with the adsorbent layer facing said one side wall, the height of said one side wall being adapted to the position of the strip of sample so that said strip will be situated somewhat below the top edge of said wall, said wall and the opposite side wall being perforated near the bottom of the trough.
Claim 7
Dependent Claim
103 words
Apparatus for carrying out the method according to claim 1, characterized in that it includes a. a bridge having longitudinal sides and a bottom, said bottom being provided with a narrow longitudinal slot, b. a holder for cutting blades, which holder is designed for cutting out a groove in the adsorbent layer by following the slot of the bridge c. an applicator designed for dispensing the sample in the groove cut in the adsorbent layer, and d. a press of T-shaped cross-section, the stem of which is arranged to fit into the slot of the bridge for compressing the sample in the groove.
Claim 8
Dependent Claim
36 words
Apparatus according to claim 6, wherein the blade holder is provided with means for scraping clean the groove in the adsorbent layer when said holder is moved to and fro along the slot in the bridge.
Claim 9
Dependent Claim
49 words
Apparatus according to claim 6, wherein the interior of the applicator has the shape of a funnel and has an outlet arranged in its base, said outlet being arranged to register with the slot of the bridge when the applicator is placed between the longitudinal sides of the bridge.
Claim 10
Dependent Claim
26 words
Apparatus according to claim 8, wherein two wedge-shaped levelling means are arranged on opposite sides of the outlet for levelling of the sample in the groove.
Claim 11
Dependent Claim
14 words
Apparatus according to claim 6, wherein the T-shaped press is provided with finger grips.
Claim 12
Dependent Claim
24 words
Apparatus according to claim 6, wherein the T-stem of the T-shaped press projects somewhat further than the thickness of the bottom of the bridge.
Claim 13
Dependent Claim
84 words
Apparatus according to claim 6, characterized in that it also includes e. a trough having vertical side and end walls and a bottom, at least one of the side walls being perforated near said bottom (34), f. an insert which rests on the bottom of the trough and is shaped to provide a plurality of equally high elevations, and g. means for securing a base plate with an adsorbent layer applied thereon in close contact with one of the side walls of the trough.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-27 | 1979-04-28 | 1979-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-27 | 1983-04-28 | 1983-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-27 | 1987-04-28 | 1987-10-28 | $7700.00 | Window Closed / Lapsed |