Abstract A method for determining the effectiveness of an interruption in the vasa deferentia or the effectiveness of the production of aspermia, total or partial inhibition of spermatogenesis (sperm production in the testes) or sperm transport through the epididymides or vasa deferentia by chemical (drug) or other (for example, radiological or immunological or agglutination or temperature) means by reacting semen with an agent or combination of agents which respond to visually or photometrically indicate the presence of sperm in the semen or the presence in the semen of a fluid which carries sperm, if any, and which can only be present if the vas interruption is incomplete. A device which is used according to the method for determining the presence of sperm includes a filter which carries (e.g. by impregnation or coating) an agent or combination of agents which react to indicate the presence of DNA, so that if sperm is present in the semen the DNA therein will provide through the reaction of the agent a visual or photometric indication of the presence of sperm, and/or an agent (or agents) which react with specific constituents in the fluids secreted or produced by the testes, the epididymides, or the vasa deferentia (for example, glycerylphosphorylcholine or glycosidases or carnitine) to give through the reaction a visual or photometric indication of the presence of these constituents in the semen.
A MOTHOD FOR DETERMINING THE EFFECTIVENESS OF AN INTERRUPTION IN THE VASA DEFERENTIAL, COMPRISING REACTING THE SEMEN WITH AGLYCODIDE SELECTED FROM THE GROUP CONSISTING OF GLUCOSIDE AND MANNOSIDE TO DETERMINE THE PRESENCE OR ABSENCE THEREIN OF THE CORRESPONDING GLYCOSIDASE ENZYMES WHICH ARE CONTAINED IN AFLUID WHICH NORMALLY CARRIES SPERM FROM THE VAS, THE ABSENCE OF SUCH GLYCOSIDASE ENZYMES CONFIRMING THE EFFECTIVENESS OFTHE VASA INTERRUPTION.
Claim 2
Dependent Claim
64 words
A MOTHOD FOR DETERMINING THE EFFECTIVENESS OF AN INTERRUPTION IN THE VASA DEFERENTIAL, COMPRISING REACTING THE SEMEN WITH AGLYCODIDE SELECTED FROM THE GROUP CONSISTING OF GLUCOSIDE AND MANNOSIDE TO DETERMINE THE PRESENCE OR ABSENCE THEREIN OF THE CORRESPONDING GLYCOSIDASE ENZYMES WHICH ARE CONTAINED IN AFLUID WHICH NORMALLY CARRIES SPERM FROM THE VAS, THE ABSENCE OF SUCH GLYCOSIDASE ENZYMES CONFIRMING THE EFFECTIVENESS OFTHE VASA INTERRUPTION.
Claim 3
Dependent Claim
34 words
The method of claim 1 and wherein said glycoside is linked to a coloring agent which upon reaction of said glycoside with the corresponding glycosidase enzyme visually indicates the presence of said glycosidase enzyme.
Claim 4
Dependent Claim
20 words
The method of claim 1 and wherein said glycoside is mannoside which reacts with a mannosidase enzyme in the fluid.
Claim 5
Dependent Claim
40 words
The method of claim 1 wherein the semen is collected in a container to which said glycoside is added to contact the semen and react with said glycosidase enzymes in said fluid if the latter is present in the semen.