US3909358A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Insolubilized enzymes
Assignee: United States Department of Agriculture
•
Filing: 1974-04-29
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Granted: 1975-09-30
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Expires: 1994-04-29
Abstract
Abstract Insolubilized but active enzymes are prepared by reacting an enzyme with chitin and glutaraldehyde.
Inventors
- 👤 William L Stanley
- 👤 Glenn G Watters
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:53
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
58 words
A PROCESS FOR PREPARING AN INSOLUBLE BUT ACTIVE ENZYME, WHICH COMPRISES: A. DISSOLVING A SOLUBLE ENZYME IN WATER, B. MIXING THE RESULTING SOLUTION WITH GRANULES OF CHITIN TO CAUSE THE ENZYME TO BE ABSORBED BY THE CHITIN, C. CONTACTING THE CHITIN CNTACING ABSORBED ENZYME WITH AN AQUEOUS SOLUTION OF GLUTARALDEHYDE, AND D. SEPARATING THE RESULTING ENZYME-CHITINGLUTARALDEHYDE REACTION PRODUCT.
Claim 2
Dependent Claim
58 words
A PROCESS FOR PREPARING AN INSOLUBLE BUT ACTIVE ENZYME, WHICH COMPRISES: A. DISSOLVING A SOLUBLE ENZYME IN WATER, B. MIXING THE RESULTING SOLUTION WITH GRANULES OF CHITIN TO CAUSE THE ENZYME TO BE ABSORBED BY THE CHITIN, C. CONTACTING THE CHITIN CNTACING ABSORBED ENZYME WITH AN AQUEOUS SOLUTION OF GLUTARALDEHYDE, AND D. SEPARATING THE RESULTING ENZYME-CHITINGLUTARALDEHYDE REACTION PRODUCT.
Claim 3
Dependent Claim
17 words
An insoluble but active enzyme comprising a complex of a soluble enzyme and chitin, cross-linked with glutaraldehyde.
Claim 4
Dependent Claim
17 words
An insoluble but active enzyme comprising a complex of a soluble enzyme and chitin, cross-linked with glutaraldehyde.
Claim 5
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a sugar-hydrolyzing enzyme.
Claim 6
Dependent Claim
10 words
The product of claim 2 wherein the enzyme is lactase.
Claim 7
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a glucose-oxidizing enzyme.
Claim 8
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a protein-degrading enzyme.
Claim 9
Dependent Claim
13 words
The product of claim 2 wherein the enzyme is a hydrogen peroxide-degrading enzyme.
Claim 10
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a starch-hydrolyzing enzyme.
References Cited (Backward Citations)
US3767531A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-29 | 1979-03-31 | 1979-09-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-29 | 1983-03-31 | 1983-09-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-29 | 1987-03-31 | 1987-09-30 | $7700.00 | Window Closed / Lapsed |