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US3909356A Expired / Lapsed US ⏱️ 0.0 years remaining

Production of fatty acid esters of fructose

Assignee: KH Neochem Co Ltd
•
Filing: 1974-08-26
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Granted: 1975-09-30
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Expires: 1994-08-26
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Abstract

Abstract A process for producing fatty acid esters of fructose including the steps of (a) culturing a fructose-utilizing microorganism capable of producing fatty acid esters of fructose and belonging to the genus Arthrobacter, Corynebacterium, Nocardia or Mycobacterium in a medium containing fructose as a carbon source, (b) forming the fatty acid esters of fructose intracellularly and (c) recovering the esters from the microbial cells.

Inventors

  • 👤 Takeo Suzuki
  • 👤 Seiga Ito

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-26 11:16
CPC Classifications
C; C07; C07H; C07H13/00; C07H13/02; C07H13/04; C07H13/06; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P19/00; C12P7/00; C12P7/62; C12R; C12R2001/00; C12R2001/01; C12R2001/06; C12R2001/15; C12R2001/29; C12R2001/31; C12R2001/32; C12R2001/34; C12R2001/365; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/843; Y10S435/863; Y10S435/866; Y10S435/867; Y10S435/869; Y10S435/872

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 56 words
A PROCESS FOR PRODUCING FATTY ACID ESTERS OF FRUCTOSE, WHCH COMPRISES CULTURING A FRUCTOSE-UTILIZING MICROORGANISM CAPABLE OF PRODUCING FATTY ACID ESTERS OF FRUCTOSE AND BELONGING TO THE GENUS ARTHROBACTER, CORYNBACTERIUM, NACARDIA OR MYCOBACTERIUM IN A MEDIUM CONTAINING FRUCTOSE INTRACELLULARLY SOURCE, FORMING THE FATTY ACID ESTERS OF FRUCTOSE INTRACELLULARLY AND RECOVERING THE ESTERS FROM THE MICROBIAL CELLS.
Claim 2
Dependent Claim 56 words
A PROCESS FOR PRODUCING FATTY ACID ESTERS OF FRUCTOSE, WHCH COMPRISES CULTURING A FRUCTOSE-UTILIZING MICROORGANISM CAPABLE OF PRODUCING FATTY ACID ESTERS OF FRUCTOSE AND BELONGING TO THE GENUS ARTHROBACTER, CORYNBACTERIUM, NACARDIA OR MYCOBACTERIUM IN A MEDIUM CONTAINING FRUCTOSE INTRACELLULARLY SOURCE, FORMING THE FATTY ACID ESTERS OF FRUCTOSE INTRACELLULARLY AND RECOVERING THE ESTERS FROM THE MICROBIAL CELLS.
Claim 3
Dependent Claim 26 words
The process of claim 1, wherein the culturing is carried out under aerobic conditions at 20*C to 40*C at a pH of from 4 to 9.
Claim 4
Dependent Claim 53 words
The process of claim 1, wherein the esters are recovered from the microbial cells by initially separating the microbial cells from the culture liquor, extracting the cells with an organic solvent system, vaporizing the solvent system, further extracting the resulting residue with another solvent and finally effecting elution of the fatty acid esters.
Claim 5
Dependent Claim 39 words
The process of claim 3, wherein elution is effected with a mixture of a chlorinated hydrocarbon and a lower aliphatic alcohol, the mixing ratio by volume of the hydrocarbon to the alcohol being stepwise varied from 99:1 to 95:5.
Claim 6
Dependent Claim 42 words
The process of claim 1, wherein the fatty acid esters obtained are a reaction product of 1 mole of fructofuranose and 1, 2, 4 or 5 mols of a fatty acid, the fatty acid residue in the esters having the following formula:
Claim 7
Dependent Claim 59 words
The process of claim 1, wherein the microorganism is selected from the group consisting of: Arthrobacter paraffineus ATCC 15591 Arthrobacter hydrocarboglutamicus ATCC 15583 Corynebacterium hydrocarboclastus ATCC 21628 Corynebacterium psudodiphtheriticum ATCC 10701 Nocardia praffinica ATCC 21198 Nocardia globerula ATCC 13130 Nocardia convoluta ATCC 4275 Mycobacterium rubrum ATCC 14346 Mycobacterium paraffinicum ATCC 12670 Mycobacterium smegmatis ATCC 21293 Mycobacterium smegmatis ATCC 607

References Cited (Backward Citations)

US3637461A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1978-09-29 1979-03-31 1979-09-30 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1982-09-29 1983-03-31 1983-09-30 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1986-09-29 1987-03-31 1987-09-30 $7700.00 Window Closed / Lapsed

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