US3898128A
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Process for preparing L-alanine
Assignee: Tanabe Pharma Corp
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Filing: 1973-11-15
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Granted: 1975-08-05
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Expires: 1993-11-15
Abstract
Abstract At least one monomer selected from the group consisting of acrylamide, N,N''-lower alkylene-bis(acrylamide) and bis(acrylamidomethyl)ether is polymerized in an aqueous suspension containing an L-aspartic acid Beta -decarboxylaseproducing microorganism. The resultant immobilised L-aspartic acid Beta -decarboxylase-producing microorganism is subjected to enzymatic reaction with L-aspartic acid, DL-aspartic acid or a salt thereof. Pyridoxal phosphate, a divalent metal ion and/or a surface active agent are preferably added to the enzymatic reaction solution. On using L-aspartic acid or its salt as substrate, L-alanine is obtained. Alternatively, on using DLaspartic acid or its salt as substrate, L-alanine and D-aspartic acid are obtained.
Inventors
- 👤 Ichiro Chibata
- 👤 Tetsuya Tosa
- 👤 Tadashi Sato
- 👤 Kozo Yamamoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:55
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/06; C12N; C12N9/00; C12N9/88; C12P13/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
86 words
A PROCESS FOR PREPARING L-ALANINE OR A MIXTURE OF L-ALANINE AND D-ASPARTIC ACID WHICH COMPRISES POLYMERIZING AT LEAST ONE MONOMER SELECTED FROM THE GROUP CONSISTING OF ACRYLAMINE N,N''-LOWER ALKYLENE-BIS (ACRYLAMIDE) AND BIS (ACRYLAMIDOMETHY) ETHER IN AN AQUEOUS SUSPENSION OF AN L ASPARTIC ACID B-DECARBOXYLASE PRODUCING MICROORGANISM TO PRODUCE AN IMMOBILIZED L-ASPARTIC ACID B-DECARBOXYLASE PRODUCING MICROORGANISM AND SUBJECTING THE IMMOBILIZED LASPARTIC ACID B-DECARBOXYLASE-PRODUCING MICROORGANISM TO ENZYMATIC REACTION WITH L-ASPARTIC ACID DL-ASPARTIC ACID OR A SALT THEREOF IN THE PRESENCE OF 0.1 TO 10 MILIMOLES/LITER OF CO++OR NI++-ION.
Claim 2
Dependent Claim
86 words
A PROCESS FOR PREPARING L-ALANINE OR A MIXTURE OF L-ALANINE AND D-ASPARTIC ACID WHICH COMPRISES POLYMERIZING AT LEAST ONE MONOMER SELECTED FROM THE GROUP CONSISTING OF ACRYLAMINE N,N''-LOWER ALKYLENE-BIS (ACRYLAMIDE) AND BIS (ACRYLAMIDOMETHY) ETHER IN AN AQUEOUS SUSPENSION OF AN L ASPARTIC ACID B-DECARBOXYLASE PRODUCING MICROORGANISM TO PRODUCE AN IMMOBILIZED L-ASPARTIC ACID B-DECARBOXYLASE PRODUCING MICROORGANISM AND SUBJECTING THE IMMOBILIZED LASPARTIC ACID B-DECARBOXYLASE-PRODUCING MICROORGANISM TO ENZYMATIC REACTION WITH L-ASPARTIC ACID DL-ASPARTIC ACID OR A SALT THEREOF IN THE PRESENCE OF 0.1 TO 10 MILIMOLES/LITER OF CO++OR NI++-ION.
Claim 3
Dependent Claim
36 words
The process of claim 1 in which the microorganism is Acetobacter rancens O.U.T. No. 8300, Achromobacter pestifer I.A.M. No. 1446, Achromobacter pestifer A.T.C.C. No. 23584, Alcaligenes faecalis A.T.C.C. No. 25094, or pseudomonas dacunhae I.A.M. No. 1152.
Claim 4
Dependent Claim
18 words
The process according to claim 1, wherein the L-aspartic acid Beta -decarboxylase-producing microorganism is Pseudomonas dacunhae IAM 1152.
Claim 5
Dependent Claim
34 words
The process according to claim 1, wherein the salt of L- or DL-aspartic acid is selected from the group consisting of ammonium L- or DL-aspartate, sodium L- or DL-aspartate and magnesium L- or Dl-aspartate.
Claim 6
Dependent Claim
24 words
The process according to claim 1, wherein the enzymatic reaction is carried out in the presence of 0.05 to 10 milimoles/liter of pyriodoxal phosphate.
Claim 7
Dependent Claim
27 words
The process according to claim 1, wherein the enzymatic reaction is carried out in the presence of 0.05 to 1.0 w/v percent of a surface active agent.
Claim 8
Dependent Claim
46 words
The process according to claim 1, wherein the polymerization reaction is carried out at 5* to 60*C in the presence of a polymerization initiator and a polymerization accelerator, and the enzymatic reaction is carried out at 0* to 60*C at a pH of 4 to 9.
Claim 9
Dependent Claim
41 words
The process according to claim 7, wherein the polymerization initiator is selected from the group consisting of potassium persulfate, ammonium persulfate, vitamin B2 and methylene Blue, and the polymerization accelerator is selected from the group consisting of Beta -(dimethylamino)-propionitrile and N,N,N'',N''-tetramethyl-ethylenediamine.
Claim 10
Dependent Claim
18 words
The process according to claim 8, wherein the L-aspartic acid Beta -decarboxylase-producing microorganism is Pseudomonas dacunhae IAM 1152.
Claim 11
Dependent Claim
38 words
The process according to claim 8, wherein the salt of L- or DL-aspartic acid is selected from the group consisting of ammonium L- or DL-aspartate, potassium L- or DL-aspartate, sodium L- or DL-aspartate and magnesium L- or DL-aspartate.
Claim 12
Dependent Claim
24 words
The process according to claim 8, wherein the enzymatic reaction is carried out in the presence of 0.05 to 10 milimoles of pyridoxal phosphate.
Claim 13
Dependent Claim
27 words
The process according to claim 8, wherein the enzymatic reaction is carried out in the presence of 0.05 to 1.0 w/v percent of a surface active agent.
Claim 14
Dependent Claim
40 words
The process according to claim 8, wherein the polymerization initiator is selected from the group consisting of potassium persulfate, ammonium persulfate, vitamin B2 and methylene Blue, and the polymerization accelerator is selected from the group consisting of -(dimethylamino)-propionitrile and N,N,N'',N''-tetramethyl-ethylenediamine.
Claim 15
Dependent Claim
105 words
A process for preparing L-alanine or a mixture of L-alanine and D-aspartic acid which comprises copolymerizing acrylamide with N,N''-lower alkylene-bis (acrylamide) or bis (acrylamidomethyl) ether in an aqueous suspension of an L-aspartic acid Beta -decarboxylase-producing microorganism at 5* to 60*C in the presence of a polymerization initiator and a polymerization accelerator to produce an immobilized L-aspartic acid Beta -decarboxylase-producing microorganism, and subjecting the immobilized L-aspartic acid Beta -decarboxylase-producing microorganism to enzymatic reaction with L-aspartic acid, DL-aspartic acid or a salt thereof at 0* to 60*C at a pH of 4 to 9 in the presence of 0.1 to 10 milimoles/liter of Co or Ni -ion.
Claim 16
Dependent Claim
105 words
A process for preparing L-alanine or a mixture of L-alanine and D-aspartic acid which comprises copolymerizing acrylamide with N,N''-lower alkylene-bis (acrylamide) or bis (acrylamidomethyl) ether in an aqueous suspension of an L-aspartic acid Beta -decarboxylase-producing microorganism at 5* to 60*C in the presence of a polymerization initiator and a polymerization accelerator to produce an immobilized L-aspartic acid Beta -decarboxylase-producing microorganism, and subjecting the immobilized L-aspartic acid Beta -decarboxylase-producing microorganism to enzymatic reaction with L-aspartic acid, DL-aspartic acid or a salt thereof at 0* to 60*C at a pH of 4 to 9 in the presence of 0.1 to 10 milimoles/liter of Co or Ni -ion.
Claim 17
Dependent Claim
36 words
The process of claim 14 in which the microorganism is Acetobacter rancens O.U.T. No. 8300, Achromobacter pestifer I.A.M. No. 1446, Achromobacter pestifer A.T.C.C. No. 23584, Alcaligenes faecalis A.T.C.C. No. 25094, or Pseudomonas dacunhae I.A.M. No. 1152.
Claim 18
Dependent Claim
18 words
The process according to claim 14, wherein the L-aspartic acid Beta -decarboxylase-producing microorganism is Pseudomonas dacunhae IAM 1152.
Claim 19
Dependent Claim
38 words
The process according to claim 14, wherein the salt of L- or DL-aspartic acid is selected from the group consisting of ammonium L- or DL-aspartate, potassium L- or DL-aspartate, sodium L- or DL-aspartate and magnesium L- or DL-aspartate.
Claim 20
Dependent Claim
24 words
The process according to claim 14, wherein the enzymatic reaction is carried out in the presence of 0.05 to 10 milimoles of pyridoxal phosphate.
Claim 21
Dependent Claim
27 words
The process according to claim 14, wherein the enzymatic reaction is carried out in the presence of 0.05 to 1.0 w/v percent of a surface active agent.
Claim 22
Dependent Claim
41 words
The process according to claim 14, wherein the polymerization initiator is selected from the group consisting of potassium persulfate, ammonium persulfate, vitamin B2 and methylene Blue, and the polymerization accelerator is selected from the group consisting of Beta -(dimethylamino)-propionitrile and N,N,N'',N''-tetramethyl-ethylenediamine.
Claim 23
Dependent Claim
103 words
A process for preparing L-alanine or a mixture of L-alanine and D-aspartic acid which comprises polymerizing N,N''-lower alkylene-bis (acrylamide) or bis (acrylamidomethyl) ether in an aqueous suspension of an L-aspartic acid Beta -decarboxylase-producing microorganism at 5* to 60*C in the presence of a polymerization initiator and a polymerization accelerator to produce an immobilized L-aspartic acid Beta -decarboxylase-producing microorganism, and subjecting the immobilized L-aspartic acid Beta -decarboxylase-producing microorganism to enzymatic reaction with L-aspartic acid, DL-aspartic acid or a salt thereof at 0* to 60*C at a pH of 4 to 9 in the presence of 0.1 to 10 milimoLes/liter of Co or Ni -ion.
Claim 24
Dependent Claim
103 words
A process for preparing L-alanine or a mixture of L-alanine and D-aspartic acid which comprises polymerizing N,N''-lower alkylene-bis (acrylamide) or bis (acrylamidomethyl) ether in an aqueous suspension of an L-aspartic acid Beta -decarboxylase-producing microorganism at 5* to 60*C in the presence of a polymerization initiator and a polymerization accelerator to produce an immobilized L-aspartic acid Beta -decarboxylase-producing microorganism, and subjecting the immobilized L-aspartic acid Beta -decarboxylase-producing microorganism to enzymatic reaction with L-aspartic acid, DL-aspartic acid or a salt thereof at 0* to 60*C at a pH of 4 to 9 in the presence of 0.1 to 10 milimoLes/liter of Co or Ni -ion.
Claim 25
Dependent Claim
36 words
The process of claim 21 in which the microorganism is Acetobacter rancens O.U.T. No. 8300, Achromobacter pestifer I.A.M. No. 1446, Achromobacter pestifer A.T.C.C. No. 23584, Alcaligenes faecalis A.T.C.C. No. 25094, or Pseudomonas dacunhae I.A.M. No. 1152.
References Cited (Backward Citations)
US3791926A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-08-04 | 1979-02-03 | 1979-08-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-08-04 | 1983-02-03 | 1983-08-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-08-04 | 1987-02-03 | 1987-08-05 | $7700.00 | Window Closed / Lapsed |