US3891504A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the manufacture of zeaxanthin
Assignee: F Hoffmann La Roche AG
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Filing: 1973-09-20
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Granted: 1975-06-24
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Expires: 1993-09-20
Abstract
Abstract A process for obtaining zeaxanthin by fermentation utilizing one of two bacterial species which belong to the genus Flavobacter and which are deposited under ATCC 21,588 and ATCC 21,081.
Inventors
- 👤 Arno Johannes Schocher
- 👤 Oswald Wiss
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:02
CPC Classifications
C; C12; C12P; C12P23/00; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/85
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
43 words
A PROCESS FOR THE PREPARATION OF ZEAZANTHIN CONTAINING CELL-MASS, COMPRISING: CULTIVATING A MICROORGANISM SELECTED FROM THE GROUP CONSISTING OF FLAVOBACTER ATCC 21,081 AND FLAVOBACTOR ATCC 21,588 IN A NUTRIENE MEDIA WHICH CONTAINS A SOURCE OF ASSIMILABLE CARBON AND A SOURCE OF ASSIMILABLE NITROGEN.
Claim 2
Dependent Claim
43 words
A PROCESS FOR THE PREPARATION OF ZEAZANTHIN CONTAINING CELL-MASS, COMPRISING: CULTIVATING A MICROORGANISM SELECTED FROM THE GROUP CONSISTING OF FLAVOBACTER ATCC 21,081 AND FLAVOBACTOR ATCC 21,588 IN A NUTRIENE MEDIA WHICH CONTAINS A SOURCE OF ASSIMILABLE CARBON AND A SOURCE OF ASSIMILABLE NITROGEN.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein said nutrient media is an aqueous solution.
Claim 4
Dependent Claim
26 words
The process of claim 2 wherein said nutrient media is agitated and subjected under partial pressures of oxygen greater than ambient at a temperature of 24*C.
Claim 5
Dependent Claim
14 words
The process of claim 2 wherein said cultivation is carried out at 10*-35* C.
Claim 6
Dependent Claim
13 words
The process of claim 4 wherein said cultivation is carried out at 20*-28*C.
Claim 7
Dependent Claim
35 words
The process of claim 1 wherein said microorganism is submergedly and aerobically cultivated in an agitated nutrient solution initially at a temperature between 28* and 35*C. and subsequently at a temperature between 20* and 28*C.
Claim 8
Dependent Claim
19 words
The process of claim 1 wherein said nutrient media includes up to 10 percent by weight of a carbohydrate.
Claim 9
Dependent Claim
10 words
The process of claim 7 wherein said carbohydrate is glucose.
Claim 10
Dependent Claim
19 words
The process of claim 1 wherein said nutrient media includes up to 1 percent by weight of a phosphate.
Claim 11
Dependent Claim
11 words
The process of claim 8 wherein said phosphate is sec.potassium o-phosphate.
Claim 12
Dependent Claim
17 words
The process of claim 1 wherein a promoter of pigment formation is added to said nutrient media.
Claim 13
Dependent Claim
24 words
The process of claim 11 wherein said pigment formation promoter is selected from the group consisting of lactic acid and palmitic acid methyl ester.
Claim 14
Dependent Claim
54 words
A process for the preparation of zeaxanthin, comprising: cultivating a microorganism selected from the group consisting of Flavobacter ATCC 21,081 and Flavobacter ATCC 21,588 in a nutrient media which contains a source of assimilable carbon and a source of assimilable nitrogen to yield a zeaxanthin containing cell-mass; and separating the zeaxanthin from said cell-mass.
Claim 15
Dependent Claim
54 words
A process for the preparation of zeaxanthin, comprising: cultivating a microorganism selected from the group consisting of Flavobacter ATCC 21,081 and Flavobacter ATCC 21,588 in a nutrient media which contains a source of assimilable carbon and a source of assimilable nitrogen to yield a zeaxanthin containing cell-mass; and separating the zeaxanthin from said cell-mass.
Claim 16
Dependent Claim
17 words
The process of claim 13 wherein said microorganism is cultivated in an aqueous nutrient media at 10*-35*C.
Claim 17
Dependent Claim
39 words
The process of claim 13 wherein the zeaxanthin is separated from said cell-mass by: drying said cell-mass; pulverizing said cell-mass; digesting said cell-mass with an inert organic solvent to dissolve the zeaxanthin; and evaporating said solvent from the zeaxanthin.
References Cited (Backward Citations)
US2974044A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-23 | 1978-12-23 | 1979-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-23 | 1982-12-23 | 1983-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-23 | 1986-12-23 | 1987-06-24 | $7700.00 | Window Closed / Lapsed |