US3890200A
Expired / Lapsed
US
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Selective medium for streptococcus mutans
Assignee: ADA Forsyth Institute Inc
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Filing: 1973-06-14
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Granted: 1975-06-17
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Expires: 1993-06-14
Abstract
Abstract A selective medium was developed for the isolation of Streptococcus mutans from human dental plaque. Mitis-salivarius agar was modified by adding 0.2 units/ml bacitracin and by increasing the sucrose concentration to 20 percent. The selective agents allowed the undiminished recovery of Strep.mutans with maximum inhibition of the balance of the streptococcal flora normally encountered on this medium.
Inventors
- 👤 Harold V Jordan
- 👤 Houte Johannes Van
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 08:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/14; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/315; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/8215; Y10S435/822; Y10S435/885
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
81 words
A SELECTIVE MEDIUM COMPOSITION FOR THE GROWTH OF STREPTOCOCCUS MUTANS, WHICH COMPOSITION COMPRISES IN COMBINATION: A. A SOLID MEDIUM SELECTIVE TO INDUCE THE GROWTH OF THE STREPTOCOCCUS MUTANS; B. THE SOLID MEDIUM CONTAINING THEREIN A COMBINATION OF A I. MONO OR DI SACCHARIDE COMPOUND; AND II. A POLYPEPTIDE ANTIBIOTIC COMPOUND COMPRISING BACITRACIN, THE SACCHARIDE AND ANTIBIOTIC COMPOUNDS PRESENT IN A CONCENTRATION INSUFFICIENT TO PREVENT THE GROWTH OF STREPTOCCUS MUTANS, BUT SUFFICIENT TO PREVENT THE GROWTH IN SUFFICIENT QUANTITY OF INTERFERING MICROORGANISMS.
Claim 2
Dependent Claim
81 words
A SELECTIVE MEDIUM COMPOSITION FOR THE GROWTH OF STREPTOCOCCUS MUTANS, WHICH COMPOSITION COMPRISES IN COMBINATION: A. A SOLID MEDIUM SELECTIVE TO INDUCE THE GROWTH OF THE STREPTOCOCCUS MUTANS; B. THE SOLID MEDIUM CONTAINING THEREIN A COMBINATION OF A I. MONO OR DI SACCHARIDE COMPOUND; AND II. A POLYPEPTIDE ANTIBIOTIC COMPOUND COMPRISING BACITRACIN, THE SACCHARIDE AND ANTIBIOTIC COMPOUNDS PRESENT IN A CONCENTRATION INSUFFICIENT TO PREVENT THE GROWTH OF STREPTOCCUS MUTANS, BUT SUFFICIENT TO PREVENT THE GROWTH IN SUFFICIENT QUANTITY OF INTERFERING MICROORGANISMS.
Claim 3
Dependent Claim
19 words
The composition of claim 1 wherein the saccharide compound is a mono saccharide compound of sucrose, fructose or glucose.
Claim 4
Dependent Claim
14 words
The composition of claim 1 wherein the solid medium is a mitis-salivarius agar medium.
Claim 5
Dependent Claim
30 words
The composition of claim 1 wherein the concentration for saccharide is from about 5 to 50 percent, and the concentration of the antibiotic is from about 0.01 to 5.0 units/ml.
Claim 6
Dependent Claim
45 words
A selective medium for the growth of Streptococcus mutans, such as from human dental plaque, which medium comprises: a mitis-salivarius agar medium containing in combination a noninhibiting amount of sucrose and bacitracin, but an amount sufficient of each to inhibit the growth of interfering organisms.
Claim 7
Dependent Claim
45 words
A selective medium for the growth of Streptococcus mutans, such as from human dental plaque, which medium comprises: a mitis-salivarius agar medium containing in combination a noninhibiting amount of sucrose and bacitracin, but an amount sufficient of each to inhibit the growth of interfering organisms.
Claim 8
Dependent Claim
33 words
The medium of claim 5 wherein the bacitracin is present in an amount of from 0.05 to 0.25 units/ml and the sucrose is present in an amount of about 20 percent by weight.
Claim 9
Dependent Claim
19 words
The medium of claim 5 wherein the bacitracin is present in an amount of from 0.125 to 0.250 units/ml.
Claim 10
Dependent Claim
19 words
The medium of claim 5 wherein the sucrose is present in amounts up to about 20 percent by weight.
Claim 11
Dependent Claim
29 words
The medium of claim 5 wherein the sucrose is present in an amount of about 20 percent by weight and the bacitracin in an amount of about 0.20 units/ml.
Claim 12
Independent Claim
98 words
The method of selective growth of a streptococcus mutans, which method comprises: a. providing a medium composition which comprises: i. a medium selective to induce the growth of the streptococcus mutans; ii. the medium containing therein a combination of a: mono or di saccharide compound; and a polypeptide antibiotic compound comprising bacitracin, the saccharide and antibiotic compounds present in a concentration insufficient to prevent the growth of the Streptococcus mutans, but sufficient to prevent the growth in sufficient quantity of interfering microorganisms; b. inoculating the medium with the selected microorganism; and c. culturing such microorganism in the medium.
Claim 13
Independent Claim
98 words
The method of selective growth of a streptococcus mutans, which method comprises: a. providing a medium composition which comprises: i. a medium selective to induce the growth of the streptococcus mutans; ii. the medium containing therein a combination of a: mono or di saccharide compound; and a polypeptide antibiotic compound comprising bacitracin, the saccharide and antibiotic compounds present in a concentration insufficient to prevent the growth of the Streptococcus mutans, but sufficient to prevent the growth in sufficient quantity of interfering microorganisms; b. inoculating the medium with the selected microorganism; and c. culturing such microorganism in the medium.
Claim 14
Dependent Claim
19 words
The method of claim 10 wherein the saccharide compound is a mono saccharide compound of sucrose, fructorse or glucose.
Claim 15
Dependent Claim
13 words
The method of claim 10 wherein the medium is a mitissalivarius agar medium.
Claim 16
Dependent Claim
30 words
The method of claim 10 wherein the concentration for saccharide is from about 5 to 50 percent, and the concentration of the antibiotic is from about 0.01 to 5.0 units/ml.
Claim 17
Independent Claim
74 words
A method for the growth and isolation of Streptococcus mutans, which method comprises: a. providing a medium of a modified mitis-salivarius-type agar which contains in combination a noninhibiting amount to the growth of Streptococcus mutans of sucrose and bacitracin, and an inhibiting amount to the growth of interfering organisms: b. inoculating the medium with a source of Streptococcus mutans; and c. growing and isolating the Streptococcus mutans on the medium free of interfering organisms.
Claim 18
Independent Claim
74 words
A method for the growth and isolation of Streptococcus mutans, which method comprises: a. providing a medium of a modified mitis-salivarius-type agar which contains in combination a noninhibiting amount to the growth of Streptococcus mutans of sucrose and bacitracin, and an inhibiting amount to the growth of interfering organisms: b. inoculating the medium with a source of Streptococcus mutans; and c. growing and isolating the Streptococcus mutans on the medium free of interfering organisms.
Claim 19
Dependent Claim
19 words
The method of claim 14 wherein the medium is inoculated with a sample of Streptococcus mutans from dental plaque.
Claim 20
Dependent Claim
34 words
The method of claim 14 where the sucrose is present in an amount of 15 percent up to 40 percent by weight, and the bacitracin in an amount of about 0.05 to 0.25 units/ml.
Claim 21
Dependent Claim
30 words
The method of claim 14 wherein the sucrose is present in an amount of from about 20 percent by weight, and the bacitracin in an amount of about 0.2 units/ml.
Claim 22
Dependent Claim
40 words
The method of claim 14 which includes determining the extent of growth of Streptococcus mutans to provide an indication of the susceptibility of the patient to caries by culturing a sample from any part of the oral cavity, including saliva.
Claim 23
Dependent Claim
39 words
The method of claim 10 which includes obtaining a sample from the oral cavity of a patient and inoculating the medium with such sample, aNd identifying the presence of Streptococcus mutans from the growth of such microorganism after culturing.
Claim 24
Dependent Claim
14 words
The method of claim 19 wherein the sample is obtained from human dental placque.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-16 | 1978-12-16 | 1979-06-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-16 | 1982-12-16 | 1983-06-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-16 | 1986-12-16 | 1987-06-17 | $7700.00 | Window Closed / Lapsed |